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Q81QR8

- Q81QR8_BACAN

UniProt

Q81QR8 - Q81QR8_BACAN

Protein

Methylisocitrate lyase

Gene

prpB

Organism
Bacillus anthracis
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate.UniRule annotation

    Catalytic activityi

    (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531Pyruvate 2Imported
    Binding sitei92 – 921Pyruvate 1Imported
    Binding sitei224 – 2241Pyruvate 6Imported

    GO - Molecular functioni

    1. methylisocitrate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. propionate catabolic process, 2-methylcitrate cycle Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported

    Keywords - Ligandi

    PyruvateImported

    Enzyme and pathway databases

    BioCyciBANT260799:GJAJ-2256-MONOMER.
    BANT261594:GJ7F-2334-MONOMER.
    UniPathwayiUPA00946.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylisocitrate lyaseUniRule annotation (EC:4.1.3.30UniRule annotation)
    Gene namesi
    Name:prpBImported
    Ordered Locus Names:BA_2350Imported, BAS2190Imported, GBAA_2350Imported
    OrganismiBacillus anthracisImported
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000005639: Chromosome, UP000000594: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi198094.BA_2350.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IQDX-ray2.00A/B1-302[»]
    4IQEX-ray2.50A/B1-302[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ81QR8.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 533Pyruvate 1 bindingImported
    Regioni76 – 772Pyruvate 4 bindingImported
    Regioni77 – 804Pyruvate 3 bindingImported

    Sequence similaritiesi

    Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000220041.
    KOiK01003.
    OMAiCADEMVD.
    OrthoDBiEOG6BGP03.

    Family and domain databases

    Gene3Di3.20.20.60. 1 hit.
    InterProiIPR018523. Isocitrate_lyase_ph_CS.
    IPR012695. PrpB.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02317. prpB. 1 hit.
    PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81QR8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAWVVNKQST QEELANRFRA LVEANEILQI PGAHDAMAAL VARNTGFLAL    50
    YLSGAAYTAS KGLPDLGIVT STEVAERARD LVRATDLPVL VDIDTGFGGV 100
    LNVARTAVEM VEAKVAAVQI EDQQLPKKCG HLNGKKLVTT EELVQKIKAI 150
    KEVAPSLYIV ARTDARGVEG LDEAIERANA YVKAGADAIF PEALQSEEEF 200
    RLFNSKVNAP LLANMTEFGK TPYYSAEEFA NMGFQMVIYP VTSLRVAAKA 250
    YENVFTLIKE TGSQKDALSN MQTRSELYET ISYHDFEELD TGIAKTVLSE 300
    DQ 302
    Length:302
    Mass (Da):33,016
    Last modified:June 1, 2003 - v1
    Checksum:iFFCD10E8A9CB1F7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP26219.1.
    AE017334 Genomic DNA. Translation: AAT31469.1.
    AE017225 Genomic DNA. Translation: AAT54502.1.
    RefSeqiNP_844733.1. NC_003997.3.
    YP_018994.1. NC_007530.2.
    YP_028451.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP26219; AAP26219; BA_2350.
    AAT31469; AAT31469; GBAA_2350.
    AAT54502; AAT54502; BAS2190.
    GeneIDi1089117.
    2819611.
    2849519.
    KEGGiban:BA_2350.
    bar:GBAA_2350.
    bat:BAS2190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP26219.1 .
    AE017334 Genomic DNA. Translation: AAT31469.1 .
    AE017225 Genomic DNA. Translation: AAT54502.1 .
    RefSeqi NP_844733.1. NC_003997.3.
    YP_018994.1. NC_007530.2.
    YP_028451.1. NC_005945.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IQD X-ray 2.00 A/B 1-302 [» ]
    4IQE X-ray 2.50 A/B 1-302 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198094.BA_2350.

    Protocols and materials databases

    DNASUi 1089117.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP26219 ; AAP26219 ; BA_2350 .
    AAT31469 ; AAT31469 ; GBAA_2350 .
    AAT54502 ; AAT54502 ; BAS2190 .
    GeneIDi 1089117.
    2819611.
    2849519.
    KEGGi ban:BA_2350.
    bar:GBAA_2350.
    bat:BAS2190.

    Phylogenomic databases

    HOGENOMi HOG000220041.
    KOi K01003.
    OMAi CADEMVD.
    OrthoDBi EOG6BGP03.

    Enzyme and pathway databases

    UniPathwayi UPA00946 .
    BioCyci BANT260799:GJAJ-2256-MONOMER.
    BANT261594:GJ7F-2334-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q81QR8.

    Family and domain databases

    Gene3Di 3.20.20.60. 1 hit.
    InterProi IPR018523. Isocitrate_lyase_ph_CS.
    IPR012695. PrpB.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR02317. prpB. 1 hit.
    PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AmesImported, Ames ancestor and Sterne.
    2. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate PortonImported, Ames ancestorImported, Ames AncestorImported and SterneImported.
    4. Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G.
      Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: AmesImported.
    5. "Crystal Structure of Carboxyvinyl-Carboxyphosphonate Phosphorylmutase from Bacillus anthracis str. Ames Ancestor."
      Center for Structural Genomics of Infectious Diseases (CSGID)
      Kim Y., Maltseva N., Kwon K, Anderson W.F., Joachimiak A.
      Submitted (JAN-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).
    6. "Crystal Structure of Carboxyvinyl-Carboxyphosphonate Phosphorylmutase from Bacillus anthracis."
      Center for Structural Genomics of Infectious Diseases (CSGID)
      Kim Y., Maltseva N., Kwon K, Anderson W.F., Joachimiak A.
      Submitted (JAN-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH PYRUVATE.

    Entry informationi

    Entry nameiQ81QR8_BACAN
    AccessioniPrimary (citable) accession number: Q81QR8
    Secondary accession number(s): E9R2D6
    , E9R2D7, Q6HYY7, Q6KSY6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3