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Protein

Methylisocitrate lyase

Gene

prpB

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate.UniRule annotation

Catalytic activityi

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531Pyruvate 2Combined sources
Binding sitei92 – 921Pyruvate 1Combined sources
Binding sitei224 – 2241Pyruvate 6Combined sources

GO - Molecular functioni

  1. methylisocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. propionate catabolic process, 2-methylcitrate cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Keywords - Ligandi

PyruvateCombined sources

Enzyme and pathway databases

BioCyciBANT260799:GJAJ-2256-MONOMER.
BANT261594:GJ7F-2334-MONOMER.
UniPathwayiUPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylisocitrate lyaseUniRule annotation (EC:4.1.3.30UniRule annotation)
Gene namesi
Name:prpBImported
Synonyms:yqiQImported
Ordered Locus Names:BA_2350Imported, BAS2190Imported, GBAA_2350Imported
ORF Names:BACvac02_2468Imported, BAHan_2517Imported, DH23_2274Imported, DJ42_691Imported, DJ43_1552Imported, DJ44_550Imported, DJ45_3577Imported, DJ48_1925Imported, DJ49_2664Imported, HYU01_11705Imported, NX97_15670Imported, OY20_15245Imported, OY21_17540Imported, OY22_06105Imported, OY23_07180Imported, OY24_16165Imported, OY25_07345Imported, OY26_14545Imported, OY27_11350Imported, OY28_05275Imported, OY29_18785Imported, OY30_25085Imported, OY31_25450Imported, OY32_20320Imported, OY33_13385Imported, OY34_12695Imported, OY35_10640Imported, OY37_22965Imported, OY38_25255Imported, OY39_08105Imported, OY40_15265Imported, RT54_11605Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_2350.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IQDX-ray2.00A/B1-302[»]
4IQEX-ray2.50A/B1-302[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81QR8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 533Pyruvate 1 bindingCombined sources
Regioni76 – 772Pyruvate 4 bindingCombined sources
Regioni77 – 804Pyruvate 3 bindingCombined sources

Sequence similaritiesi

Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220041.
KOiK01003.
OMAiEEHYRAF.
OrthoDBiEOG6BGP03.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR018523. Isocitrate_lyase_ph_CS.
IPR012695. PrpB.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02317. prpB. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81QR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWVVNKQST QEELANRFRA LVEANEILQI PGAHDAMAAL VARNTGFLAL
60 70 80 90 100
YLSGAAYTAS KGLPDLGIVT STEVAERARD LVRATDLPVL VDIDTGFGGV
110 120 130 140 150
LNVARTAVEM VEAKVAAVQI EDQQLPKKCG HLNGKKLVTT EELVQKIKAI
160 170 180 190 200
KEVAPSLYIV ARTDARGVEG LDEAIERANA YVKAGADAIF PEALQSEEEF
210 220 230 240 250
RLFNSKVNAP LLANMTEFGK TPYYSAEEFA NMGFQMVIYP VTSLRVAAKA
260 270 280 290 300
YENVFTLIKE TGSQKDALSN MQTRSELYET ISYHDFEELD TGIAKTVLSE

DQ
Length:302
Mass (Da):33,016
Last modified:June 1, 2003 - v1
Checksum:iFFCD10E8A9CB1F7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP26219.1.
AE017334 Genomic DNA. Translation: AAT31469.1.
AE017225 Genomic DNA. Translation: AAT54502.1.
CP008846 Genomic DNA. Translation: AIF56568.1.
CP007660 Genomic DNA. Translation: AIK08399.1.
CP007618 Genomic DNA. Translation: AIK30537.1.
CP008752 Genomic DNA. Translation: AIK49853.1.
CP007704 Genomic DNA. Translation: AIK55304.1.
CP008853 Genomic DNA. Translation: AIM06178.1.
CP008854 Genomic DNA. Translation: AIM11604.1.
CP010342 Genomic DNA. Translation: AJA86586.1.
JNOD01000013 Genomic DNA. Translation: KFJ82195.1.
JMPV01000008 Genomic DNA. Translation: KFL67977.1.
JMPU01000016 Genomic DNA. Translation: KFL68166.1.
JTAQ01000028 Genomic DNA. Translation: KGZ46478.1.
JTAS01000050 Genomic DNA. Translation: KGZ52547.1.
JTAR01000023 Genomic DNA. Translation: KGZ53452.1.
JSZR01000024 Genomic DNA. Translation: KGZ66601.1.
JSZQ01000036 Genomic DNA. Translation: KGZ68492.1.
JSZP01000022 Genomic DNA. Translation: KGZ71468.1.
JSZS01000040 Genomic DNA. Translation: KGZ79517.1.
JSZT01000035 Genomic DNA. Translation: KGZ85446.1.
JSZU01000018 Genomic DNA. Translation: KGZ87753.1.
JSZW01000042 Genomic DNA. Translation: KGZ93939.1.
JSZV01000039 Genomic DNA. Translation: KGZ95104.1.
JSZY01000050 Genomic DNA. Translation: KGZ97944.1.
JTAB01000044 Genomic DNA. Translation: KHA14146.1.
JTAA01000027 Genomic DNA. Translation: KHA14735.1.
JTAD01000041 Genomic DNA. Translation: KHA22906.1.
JTAE01000054 Genomic DNA. Translation: KHA24033.1.
JTAG01000058 Genomic DNA. Translation: KHA41025.1.
JTAF01000019 Genomic DNA. Translation: KHA42571.1.
JSZX01000035 Genomic DNA. Translation: KHG44898.1.
JSZZ01000036 Genomic DNA. Translation: KHG51246.1.
JTAP01000034 Genomic DNA. Translation: KHG61212.1.
RefSeqiNP_844733.1. NC_003997.3.
WP_000312652.1. NZ_KN050651.1.
YP_018994.1. NC_007530.2.
YP_028451.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP26219; AAP26219; BA_2350.
AAT31469; AAT31469; GBAA_2350.
AAT54502; AAT54502; BAS2190.
GeneIDi1089117.
2819611.
2849519.
KEGGiban:BA_2350.
bar:GBAA_2350.
bat:BAS2190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP26219.1.
AE017334 Genomic DNA. Translation: AAT31469.1.
AE017225 Genomic DNA. Translation: AAT54502.1.
CP008846 Genomic DNA. Translation: AIF56568.1.
CP007660 Genomic DNA. Translation: AIK08399.1.
CP007618 Genomic DNA. Translation: AIK30537.1.
CP008752 Genomic DNA. Translation: AIK49853.1.
CP007704 Genomic DNA. Translation: AIK55304.1.
CP008853 Genomic DNA. Translation: AIM06178.1.
CP008854 Genomic DNA. Translation: AIM11604.1.
CP010342 Genomic DNA. Translation: AJA86586.1.
JNOD01000013 Genomic DNA. Translation: KFJ82195.1.
JMPV01000008 Genomic DNA. Translation: KFL67977.1.
JMPU01000016 Genomic DNA. Translation: KFL68166.1.
JTAQ01000028 Genomic DNA. Translation: KGZ46478.1.
JTAS01000050 Genomic DNA. Translation: KGZ52547.1.
JTAR01000023 Genomic DNA. Translation: KGZ53452.1.
JSZR01000024 Genomic DNA. Translation: KGZ66601.1.
JSZQ01000036 Genomic DNA. Translation: KGZ68492.1.
JSZP01000022 Genomic DNA. Translation: KGZ71468.1.
JSZS01000040 Genomic DNA. Translation: KGZ79517.1.
JSZT01000035 Genomic DNA. Translation: KGZ85446.1.
JSZU01000018 Genomic DNA. Translation: KGZ87753.1.
JSZW01000042 Genomic DNA. Translation: KGZ93939.1.
JSZV01000039 Genomic DNA. Translation: KGZ95104.1.
JSZY01000050 Genomic DNA. Translation: KGZ97944.1.
JTAB01000044 Genomic DNA. Translation: KHA14146.1.
JTAA01000027 Genomic DNA. Translation: KHA14735.1.
JTAD01000041 Genomic DNA. Translation: KHA22906.1.
JTAE01000054 Genomic DNA. Translation: KHA24033.1.
JTAG01000058 Genomic DNA. Translation: KHA41025.1.
JTAF01000019 Genomic DNA. Translation: KHA42571.1.
JSZX01000035 Genomic DNA. Translation: KHG44898.1.
JSZZ01000036 Genomic DNA. Translation: KHG51246.1.
JTAP01000034 Genomic DNA. Translation: KHG61212.1.
RefSeqiNP_844733.1. NC_003997.3.
WP_000312652.1. NZ_KN050651.1.
YP_018994.1. NC_007530.2.
YP_028451.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IQDX-ray2.00A/B1-302[»]
4IQEX-ray2.50A/B1-302[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_2350.

Protocols and materials databases

DNASUi1089117.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP26219; AAP26219; BA_2350.
AAT31469; AAT31469; GBAA_2350.
AAT54502; AAT54502; BAS2190.
GeneIDi1089117.
2819611.
2849519.
KEGGiban:BA_2350.
bar:GBAA_2350.
bat:BAS2190.

Phylogenomic databases

HOGENOMiHOG000220041.
KOiK01003.
OMAiEEHYRAF.
OrthoDBiEOG6BGP03.

Enzyme and pathway databases

UniPathwayiUPA00946.
BioCyciBANT260799:GJAJ-2256-MONOMER.
BANT261594:GJ7F-2334-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81QR8.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR018523. Isocitrate_lyase_ph_CS.
IPR012695. PrpB.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02317. prpB. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AmesImported and Ames / isolate PortonImported.
  2. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SterneImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestorImported and Ames AncestorImported.
  4. Joardar V., Shrivastava S., Brinkac L.M., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: AmesImported.
  5. "Crystal Structure of Carboxyvinyl-Carboxyphosphonate Phosphorylmutase from Bacillus anthracis str. Ames Ancestor."
    Center for Structural Genomics of Infectious Diseases (CSGID)
    Kim Y., Maltseva N., Kwon K., Anderson W.F., Joachimiak A.
    Submitted (JAN-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).
  6. "Crystal Structure of Carboxyvinyl-Carboxyphosphonate Phosphorylmutase from Bacillus anthracis."
    Center for Structural Genomics of Infectious Diseases (CSGID)
    Kim Y., Maltseva N., Kwon K., Anderson W.F., Joachimiak A.
    Submitted (JAN-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH PYRUVATE.
  7. Tong Y.G., Huang Y., An X.P., Li S.S., Mi Z.Q., Pei G.Q., Zhang Z.Y., Fan H.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Cvac02Imported and HanImported.
  8. "Complete Genome Sequence of Bacillus anthracis HYU01, Isolated from Soil Samples in the Korean Peninsula."
    Kim S.K., Chung W.H., Kim S.H., Jung K.H., Kim N., Chai Y.G.
    Genome Announc. 2:e00769-14(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: HYU01Imported.
  9. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031021Imported, A.Br.003Imported, Smith 1013Imported and Zimbabwe 89Imported.
  10. "Draft Genome Sequence of Bacillus anthracis STI."
    Okinaka R.T., Challacombe J.F., Drees K., Birdsell D.N., Janke N., Naumann A., Seymour M., O'Neill H.H., Schupp J., Sahl J., Foster J.T., Pearson T., Keim P.
    Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: STI-1Imported.
  11. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BFVImported.
  12. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Delta SterneImported.
  13. "Centers for Disease Control and Prevention Bacillus anthracis whole genome sequencing."
    Hoffmaster A.R., Marston C.K., Gee J., Tiller R., Beesley C.A., Bell M., Elrod M., France M., Sammons S., Rodriguez-R L.M., Weigand M.R., Konstantinidis K.T.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031006Imported, 2000031008Imported, 2000031023Imported, 2000031031Imported, 2000031038Imported, 2000031039Imported, 2000031052Imported, 2000031075Imported, 2000031709Imported, 2000031765Imported, 2000032819Imported, 2000032832Imported, 2000032879Imported, 2000032892Imported, 2000032951Imported, 2000032967Imported, 2000032975Imported, 2000032979Imported, 2000032989Imported and 2002013094Imported.
  14. "Centers for Disease Control and Prevention Bacillus anthracis whole genome sequencing."
    Hoffmaster A.R., Marston C.K., Gee J., Tiller R., Beesley C., Bell M., Elrod M., France M., Sammons S., Rodriguez-R L.M., Weigand M.R., Konstantinidis K.T.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2000031027Imported.
  15. "Multiplex PCR for Species-Level Identification of Bacillus anthracis and Detection of pXO1, pXO2, and Related Plasmids."
    Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J., Monaco A., King S., Sohrabi A.
    Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A0157Imported.

Entry informationi

Entry nameiQ81QR8_BACAN
AccessioniPrimary (citable) accession number: Q81QR8
Secondary accession number(s): E9R2D6
, E9R2D7, Q6HYY7, Q6KSY6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: March 4, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.