Reviewed, UniProtKB/Swiss-Prot Q81QB6 (IOLA2_BACAN)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 2
      Short name=MMSA dehydrogenase 2
      Short name=MMSDH 2
      Short name=MSDH 2
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 2
      Short name=MSA dehydrogenase 2
    EC=1.2.1.18

Gene names

Name:	iolA2
Ordered Locus Names:	BA_2513, GBAA_2513, BAS2334

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity. HAMAP MF_01670
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity. HAMAP MF_01670
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Methylmalonate semialdehyde dehydrogenase [acylating] 2 HAMAP MF_01670		PRO_0000352319
Regions
	Nucleotide binding	178 – 182	5	NAD By similarity
Sites
	Active site	286	1	Nucleophile By similarity
	Binding site	386	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81QB6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: EB15A1E2ED983F76	FASTA	487	53,139
	10 20 30 40 50 60 MTVQTAQIVK NYIGGEWVES ISTKMEAVYN PATGEVIAQV PLSTKVDVEQ AVLAANEAFK 70 80 90 100 110 120 SWSKTAVPRR ARILFKYQQL LVDNWEELAK LITIENGKSY NEAYGEVLRG IECVEFAAGA 130 140 150 160 170 180 PTLMMGKQLP DIATGIESGM YRYPIGVIGG ITPFNFPMMV PCWMFPLAIA CGNTFVLKPS 190 200 210 220 230 240 ERTPLLAARL AELAEEAGLP KGVLNIVNGA HDVVNGLLEH KLVKAISFVG SQPVAEYVYK 250 260 270 280 290 300 KGTENLKRVQ ALAGAKNHSI VLNDANLELA TKQIISAAFG SAGERCMAAS VVTVEEEIAD 310 320 330 340 350 360 QLVERLVAEA NKIVIGNGLD EDVFLGPVIR DNHKERTIGY IDSGVEQGAT LVRDGREDTA 370 380 390 400 410 420 VKGAGYFVGP TIFDHVTKEM KIWQDEIFAP VLSIVRVKSL DEAIEIANES RFANGACIYT 430 440 450 460 470 480 DSGASVRQFR ETIESGMLGV NVGVPAPMAF FPFSGWKDSF YGDLHANGTD GVEFYTRKKM LTSRWEK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
[3]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP26371.1. AE017334 Genomic DNA. Translation: AAT31624.1. AE017225 Genomic DNA. Translation: AAT54646.1.
RefSeq	NP_844885.1. YP_019149.1. YP_028595.1.
3D structure databases
HSSP	HSSP built from PDB template 1T90 based on UniProtKB P42412.
SMR	Q81QB6. Positions 9-485.
ModBase	Search...
Genome annotation databases
GeneID	1084066. 2815765. 2852503.
GenomeReviews	Gene locus BA_2513 in contig AE016879_GR. Gene locus BAS2334 in contig AE017225_GR. Gene locus GBAA_2513 in contig AE017334_GR.
KEGG	ban:BA2513. bar:GBAA2513. bat:BAS2334.
TIGR	BA_2513. GBAA_2513.
Phylogenomic databases
HOGENOM	HBG752218.
OMA	HIYTTAS.
Enzyme and pathway databases
BioCyc	BANT260799:BAS2334-MONOMER. BANT261594:GBAA2513-MONOMER.
Family and domain databases
HAMAP	MF_01670. IolA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA2_BACAN

Accession

Primary (citable) accession number: Q81QB6
Secondary accession number(s): Q6HYJ3, Q6KSJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)