Reviewed, UniProtKB/Swiss-Prot Q81QB5 (IOLD_BACAN)

Names and origin

Protein names

Recommended name:
    3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
      Short name=THcHDO hydrolase
    EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	BA_2514, GBAA_2514, BAS2335

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity.
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352529
Regions
	Region	442 – 522	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	493	1	Magnesium By similarity
	Metal binding	520	1	Magnesium By similarity
	Binding site	65	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81QB5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 94239E0062C6B52B	FASTA	644	71,013
	10 20 30 40 50 60 MQTVRMTTAQ ALVKFLNQQY VEFDGKQQKF VKGIFTIFGH GNVVGLGQAL EEDAGELEVY 70 80 90 100 110 120 QGRNEQGMAN AAMAFAKQKH RKQIMACTSS VGPGSANMIT SAATASANNI PVLLLPGDVF 130 140 150 160 170 180 ATRQPDPVLQ QIEQTHDLSI STNDAFRAVS KYWDRINRPE QLMTAMIQAM RVLTNPADTG 190 200 210 220 230 240 AVTICLPQDV QGEAWDFPSY FFQKRVHRIE RRLPTKASLA DAVEMIKRKK KPVMICGGGV 250 260 270 280 290 300 RYAEAAEELK QFAETFHIPF GETQAGKSAI ESSHPYNLGG IGVTGNVAAN TIAKEADLVI 310 320 330 340 350 360 GIGTRFTDFT TASKQLFQNE EVEFLNINIS EFHANKLDAL KVIADAKEAL LALIDELQEI 370 380 390 400 410 420 DYQSSYTVEI ADAKDAWETE LSRLHNIRFT CQDFTPEVEG HFNENLNEYV DALGTQLTQT 430 440 450 460 470 480 AVIGQINTLL DKDAIIVGAA GSLPGDLQRM WASRKPNTYH MEYGYSCMGY EVAGALGAKL 490 500 510 520 530 540 AEPSKEVYAM VGDGSYQMLH SELVTSLQEN KKINVLLFDN SGFGCINNLQ MGNGMGSFGT 550 560 570 580 590 600 EFRYRNQETR KLDGAIMKID FAASAAGYGV KTYHVTSLEQ LQEALIDAKK QTVSTLIDIK 610 620 630 640 VLPKTMTNGY ESWWHVGVAE VSKNQSVQAA YESKVSNLQQ ARSY

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
[3]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP26372.1. AE017334 Genomic DNA. Translation: AAT31625.1. AE017225 Genomic DNA. Translation: AAT54647.1.
RefSeq	NP_844886.1. YP_019150.1. YP_028596.1.
3D structure databases
HSSP	HSSP built from PDB template 1JSC based on UniProtKB P07342.
ModBase	Search...
Genome annotation databases
GeneID	1085050. 2815665. 2852648.
GenomeReviews	Gene locus BA_2514 in contig AE016879_GR. Gene locus BAS2335 in contig AE017225_GR. Gene locus GBAA2514 in contig AE017334_GR.
KEGG	ban:BA2514. bar:GBAA2514. bat:BAS2335.
TIGR	BA_2514. GBAA_2514.
Phylogenomic databases
HOGENOM	Q81QB5.
OMA	Q81QB5. GELEVYQ.
Family and domain databases
HAMAP	MF_01669. [Tree]
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_BACAN

Accession

Primary (citable) accession number: Q81QB5
Secondary accession number(s): Q6HYJ2, Q6KSJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)