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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi248Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 2UniRule annotation1
Metal bindingi262Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 181ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciANTHRA:DDLB-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Synonyms:ddlB
Ordered Locus Names:BA_2610, GBAA_2610, BAS2435
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001777811 – 304D-alanine--D-alanine ligaseAdd BLAST304

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_2610.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 7Combined sources7
Helixi11 – 27Combined sources17
Turni30 – 32Combined sources3
Beta strandi33 – 39Combined sources7
Helixi43 – 45Combined sources3
Helixi46 – 49Combined sources4
Turni50 – 52Combined sources3
Beta strandi54 – 58Combined sources5
Helixi63 – 66Combined sources4
Helixi69 – 77Combined sources9
Beta strandi81 – 84Combined sources4
Helixi86 – 93Combined sources8
Helixi95 – 104Combined sources10
Beta strandi112 – 119Combined sources8
Helixi123 – 129Combined sources7
Beta strandi131 – 137Combined sources7
Beta strandi142 – 144Combined sources3
Beta strandi147 – 149Combined sources3
Helixi152 – 165Combined sources14
Beta strandi167 – 173Combined sources7
Beta strandi177 – 185Combined sources9
Beta strandi193 – 202Combined sources10
Beta strandi205 – 217Combined sources13
Helixi222 – 238Combined sources17
Beta strandi243 – 252Combined sources10
Beta strandi255 – 264Combined sources10
Helixi272 – 279Combined sources8
Helixi284 – 302Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R23X-ray2.50A/B1-304[»]
3R5XX-ray2.00A/B/C/D1-304[»]
ProteinModelPortaliQ81Q29.
SMRiQ81Q29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 293ATP-graspUniRule annotationAdd BLAST195

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
HOGENOMiHOG000011592.
KOiK01921.
OMAiTKYTEGF.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81Q29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVIMGGV SSEKQVSIMT GNEMIANLDK NKYEIVPITL NEKMDLIEKA
60 70 80 90 100
KDIDFALLAL HGKYGEDGTV QGTLESLGIP YSGSNMLSSG ICMDKNISKK
110 120 130 140 150
ILRYEGIETP DWIELTKMED LNFDELDKLG FPLVVKPNSG GSSVGVKIVY
160 170 180 190 200
DKDELISMLE TVFEWDSEVV IEKYIKGEEI TCSIFDGKQL PIISIRHAAE
210 220 230 240 250
FFDYNAKYDD ASTIEEVIEL PAELKERVNK ASLACYKALK CSVYARVDMM
260 270 280 290 300
VKDGIPYVME VNTLPGMTQA SLLPKSADAA GIHYSKLLDM IIETSLRVRK

EEGF
Length:304
Mass (Da):33,852
Last modified:June 1, 2003 - v1
Checksum:iFB85F1B59BC2B9B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP26458.1.
AE017334 Genomic DNA. Translation: AAT31727.1.
AE017225 Genomic DNA. Translation: AAT54746.1.
RefSeqiNP_844972.1. NC_003997.3.
WP_003157623.1. NZ_LHUO01000023.1.
YP_028695.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP26458; AAP26458; BA_2610.
AAT31727; AAT31727; GBAA_2610.
AAT54746; AAT54746; BAS2435.
GeneIDi1086552.
2852726.
KEGGiban:BA_2610.
bar:GBAA_2610.
bat:BAS2435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP26458.1.
AE017334 Genomic DNA. Translation: AAT31727.1.
AE017225 Genomic DNA. Translation: AAT54746.1.
RefSeqiNP_844972.1. NC_003997.3.
WP_003157623.1. NZ_LHUO01000023.1.
YP_028695.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R23X-ray2.50A/B1-304[»]
3R5XX-ray2.00A/B/C/D1-304[»]
ProteinModelPortaliQ81Q29.
SMRiQ81Q29.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_2610.

Protocols and materials databases

DNASUi1086552.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP26458; AAP26458; BA_2610.
AAT31727; AAT31727; GBAA_2610.
AAT54746; AAT54746; BAS2435.
GeneIDi1086552.
2852726.
KEGGiban:BA_2610.
bar:GBAA_2610.
bat:BAS2435.

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
HOGENOMiHOG000011592.
KOiK01921.
OMAiTKYTEGF.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciANTHRA:DDLB-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDL_BACAN
AccessioniPrimary (citable) accession number: Q81Q29
Secondary accession number(s): Q6HY93, Q6KY66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.