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Q81Q29

- DDL_BACAN

UniProt

Q81Q29 - DDL_BACAN

Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Cell wall formation.UniRule annotation

    Catalytic activityi

    ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi248 – 2481Magnesium or manganese 1UniRule annotation
    Metal bindingi260 – 2601Magnesium or manganese 1UniRule annotation
    Metal bindingi260 – 2601Magnesium or manganese 2UniRule annotation
    Metal bindingi262 – 2621Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi126 – 18156ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    2. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciANTHRA:DDLB-MONOMER.
    BANT260799:GJAJ-2500-MONOMER.
    BANT261594:GJ7F-2586-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
    Alternative name(s):
    D-Ala-D-Ala ligaseUniRule annotation
    D-alanylalanine synthetaseUniRule annotation
    Gene namesi
    Name:ddlUniRule annotation
    Synonyms:ddlB
    Ordered Locus Names:BA_2610, GBAA_2610, BAS2435
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304D-alanine--D-alanine ligasePRO_0000177781Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi198094.BA_2610.

    Structurei

    Secondary structure

    304
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 77
    Helixi11 – 2717
    Turni30 – 323
    Beta strandi33 – 397
    Helixi43 – 453
    Helixi46 – 494
    Turni50 – 523
    Beta strandi54 – 585
    Helixi63 – 664
    Helixi69 – 779
    Beta strandi81 – 844
    Helixi86 – 938
    Helixi95 – 10410
    Beta strandi112 – 1198
    Helixi123 – 1297
    Beta strandi131 – 1377
    Beta strandi142 – 1443
    Beta strandi147 – 1493
    Helixi152 – 16514
    Beta strandi167 – 1737
    Beta strandi177 – 1859
    Beta strandi193 – 20210
    Beta strandi205 – 21713
    Helixi222 – 23817
    Beta strandi243 – 25210
    Beta strandi255 – 26410
    Helixi272 – 2798
    Helixi284 – 30219

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3R23X-ray2.50A/B1-304[»]
    3R5XX-ray2.00A/B/C/D1-304[»]
    ProteinModelPortaliQ81Q29.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini99 – 293195ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000011592.
    KOiK01921.
    OMAiCSGYSRV.
    OrthoDBiEOG6ND0KB.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR23132. PTHR23132. 1 hit.
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81Q29-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIGVIMGGV SSEKQVSIMT GNEMIANLDK NKYEIVPITL NEKMDLIEKA    50
    KDIDFALLAL HGKYGEDGTV QGTLESLGIP YSGSNMLSSG ICMDKNISKK 100
    ILRYEGIETP DWIELTKMED LNFDELDKLG FPLVVKPNSG GSSVGVKIVY 150
    DKDELISMLE TVFEWDSEVV IEKYIKGEEI TCSIFDGKQL PIISIRHAAE 200
    FFDYNAKYDD ASTIEEVIEL PAELKERVNK ASLACYKALK CSVYARVDMM 250
    VKDGIPYVME VNTLPGMTQA SLLPKSADAA GIHYSKLLDM IIETSLRVRK 300
    EEGF 304
    Length:304
    Mass (Da):33,852
    Last modified:June 1, 2003 - v1
    Checksum:iFB85F1B59BC2B9B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP26458.1.
    AE017334 Genomic DNA. Translation: AAT31727.1.
    AE017225 Genomic DNA. Translation: AAT54746.1.
    RefSeqiNP_844972.1. NC_003997.3.
    YP_019252.1. NC_007530.2.
    YP_028695.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP26458; AAP26458; BA_2610.
    AAT31727; AAT31727; GBAA_2610.
    AAT54746; AAT54746; BAS2435.
    GeneIDi1086552.
    2817170.
    2852726.
    KEGGiban:BA_2610.
    bar:GBAA_2610.
    bat:BAS2435.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP26458.1 .
    AE017334 Genomic DNA. Translation: AAT31727.1 .
    AE017225 Genomic DNA. Translation: AAT54746.1 .
    RefSeqi NP_844972.1. NC_003997.3.
    YP_019252.1. NC_007530.2.
    YP_028695.1. NC_005945.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3R23 X-ray 2.50 A/B 1-304 [» ]
    3R5X X-ray 2.00 A/B/C/D 1-304 [» ]
    ProteinModelPortali Q81Q29.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198094.BA_2610.

    Protocols and materials databases

    DNASUi 1086552.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP26458 ; AAP26458 ; BA_2610 .
    AAT31727 ; AAT31727 ; GBAA_2610 .
    AAT54746 ; AAT54746 ; BAS2435 .
    GeneIDi 1086552.
    2817170.
    2852726.
    KEGGi ban:BA_2610.
    bar:GBAA_2610.
    bat:BAS2435.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000011592.
    KOi K01921.
    OMAi CSGYSRV.
    OrthoDBi EOG6ND0KB.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci ANTHRA:DDLB-MONOMER.
    BANT260799:GJAJ-2500-MONOMER.
    BANT261594:GJ7F-2586-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPi MF_00047. Dala_Dala_lig.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR23132. PTHR23132. 1 hit.
    Pfami PF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.
    3. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.

    Entry informationi

    Entry nameiDDL_BACAN
    AccessioniPrimary (citable) accession number: Q81Q29
    Secondary accession number(s): Q6HY93, Q6KY66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3