Reviewed, UniProtKB/Swiss-Prot Q81P64 (AROA_BACAN)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BA_2953, GBAA_2953, BAS2744

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088218

Sequences

	Sequence		Length	Mass (Da)
	Q81P64-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4FB10E45A4A67308	FASTA	429	45,264
	10 20 30 40 50 60 MKERTIQPVN NGLNGNITIP GDKSISHRAV MFGAIAEGTT TIKGFLPGAD CLSTISCFKE 70 80 90 100 110 120 MGVDIVQNGD EVTVVGKGLE GLQEPKAVLD VGNSGTTIRL MSGILANTPF LSCVQGDTSI 130 140 150 160 170 180 AKRPMKRVTN PLKQMGANID GREEGTFTPL TIRGGDLKAI EYTSPVASAQ VKSAILLAGL 190 200 210 220 230 240 RAEGVTAVTE PHISRDHTER MLEAFGVKVT REGKTVKLAG GQKLTATDVQ VPGDVSSAAF 250 260 270 280 290 300 FLVAGAIIPN SKLVLENVGM NPTRTGIIDV LEKMGATFTV EPINEGASEP AANITIETSS 310 320 330 340 350 360 LKGIEIGGDI IPRLIDEIPV IALAATQAEG ITVIKDAHEL KVKETNRIDT VVAELTKLGA 370 380 390 400 410 420 RIEATDDRMI IYGKSALKGN TVNSYGDHRI GMMLAIAGCI AEGKTIIEDA EAVGVSYPTF FEELQKLAK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP26774.1. AE017334 Genomic DNA. Translation: AAT32071.2. AE017225 Genomic DNA. Translation: AAT55053.1.
RefSeq	NP_845288.1. YP_019596.2. YP_029002.1.
3D structure databases
HSSP	HSSP built from PDB template 1RF4 based on UniProtKB Q9S400.
ModBase	Search...
Genome annotation databases
GeneID	1086557. 2817005. 2852567.
GenomeReviews	Gene locus BA_2953 in contig AE016879_GR. Gene locus BAS2744 in contig AE017225_GR. Gene locus GBAA_2953 in contig AE017334_GR.
KEGG	ban:BA2953. bar:GBAA2953. bat:BAS2744.
TIGR	BA_2953. GBAA_2953.
Phylogenomic databases
HOGENOM	Q81P64.
OMA	NPTRIEA.
Enzyme and pathway databases
BioCyc	BANT260799:BAS2744-MON. BANT261594:GBAA2953-MON.
BRENDA	2.5.1.19. 267517.
Family and domain databases
HAMAP	MF_00210. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BACAN

Accession

Primary (citable) accession number: Q81P64
Secondary accession number(s): Q6HXD6, Q6KRF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)