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Protein

Glutamate 5-kinase

Gene

proB

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9ATPUniRule annotation1
Binding sitei49SubstrateUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Binding sitei148Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 169ATPUniRule annotation2
Nucleotide bindingi210 – 216ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:BA_2993, GBAA_2993, BAS2782
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096321 – 367Glutamate 5-kinaseAdd BLAST367

Interactioni

Protein-protein interaction databases

IntActiQ81P26, 3 interactors
STRINGi260799.BAS2782

Structurei

3D structure databases

ProteinModelPortaliQ81P26
SMRiQ81P26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini276 – 350PUAUniRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q81P26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQRIVVKI GSSSLADSHE GISKEQLSDH VAALARLKEE GHEVLLITSG
60 70 80 90 100
AVAAGFSALG YPSRPVTIKG KQAAAAVGQS LLMQAYTEEF RKYGIVTAQL
110 120 130 140 150
LLTRSDFSRK EQYSNAYATL GELLNRSALP IINENDSISL EELTFGDNDM
160 170 180 190 200
LSALVSGLVS ADMLMIFTDV NGLYDKNPQK NEDAKKYYFL PEVTEEIASL
210 220 230 240 250
AGDAGSKLGT GGMKSKVDAA KTALSLGVSV FIGTGRGQEK FVDVLKGKGD
260 270 280 290 300
GTYVGNAPQK EMKINKQWIA LHSVVSGQIE IDAGAATAII QHGKSLLPAG
310 320 330 340 350
VTNVSGFFQV GEVVEVMTQQ GRVIGKGQCT YSAEELRDVK GMQSQQIQAR
360
GERHNYEVIH RDYWVSF
Length:367
Mass (Da):39,600
Last modified:June 1, 2003 - v1
Checksum:i259620BA1F3975AD
GO

Sequence cautioni

The sequence AAT55091 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP26812.1
AE017334 Genomic DNA Translation: AAT32111.2
AE017225 Genomic DNA Translation: AAT55091.1 Different initiation.
RefSeqiNP_845326.1, NC_003997.3
WP_000744711.1, NZ_PPES01000015.1

Genome annotation databases

EnsemblBacteriaiAAP26812; AAP26812; BA_2993
AAT32111; AAT32111; GBAA_2993
AAT55091; AAT55091; BAS2782
GeneIDi1086339
KEGGiban:BA_2993
bar:GBAA_2993
PATRICifig|198094.11.peg.2974

Similar proteinsi

Entry informationi

Entry nameiPROB_BACAN
AccessioniPrimary (citable) accession number: Q81P26
Secondary accession number(s): Q6HX98, Q6KRC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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