Q81P26 (PROB_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BA_2993, GBAA_2993, BAS2782

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.
Sequence caution	The sequence AAT55091.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109632
Regions
	Domain	276 – 350	75	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q81P26 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 259620BA1F3975AD	FASTA	367	39,600
	10 20 30 40 50 60 MKKQRIVVKI GSSSLADSHE GISKEQLSDH VAALARLKEE GHEVLLITSG AVAAGFSALG 70 80 90 100 110 120 YPSRPVTIKG KQAAAAVGQS LLMQAYTEEF RKYGIVTAQL LLTRSDFSRK EQYSNAYATL 130 140 150 160 170 180 GELLNRSALP IINENDSISL EELTFGDNDM LSALVSGLVS ADMLMIFTDV NGLYDKNPQK 190 200 210 220 230 240 NEDAKKYYFL PEVTEEIASL AGDAGSKLGT GGMKSKVDAA KTALSLGVSV FIGTGRGQEK 250 260 270 280 290 300 FVDVLKGKGD GTYVGNAPQK EMKINKQWIA LHSVVSGQIE IDAGAATAII QHGKSLLPAG 310 320 330 340 350 360 VTNVSGFFQV GEVVEVMTQQ GRVIGKGQCT YSAEELRDVK GMQSQQIQAR GERHNYEVIH RDYWVSF

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP26812.1. AE017334 Genomic DNA. Translation: AAT32111.2. AE017225 Genomic DNA. Translation: AAT55091.1. Different initiation.
RefSeq	NP_845326.1. NC_003997.3. YP_019636.2. NC_007530.2.
3D structure databases
ProteinModelPortal	Q81P26.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81P26. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000012665; EBBACP00000012421; EBBACG00000012657. EBBACT00000014997; EBBACP00000014618; EBBACG00000014989. EBBACT00000024286; EBBACP00000023771; EBBACG00000024277.
GeneID	1086339. 2819367.
GenomeReviews	Gene locus BA_2993 in contig AE016879_GR. Gene locus BAS2782 in contig AE017225_GR. Gene locus GBAA_2993 in contig AE017334_GR.
KEGG	ban:BA_2993. bar:GBAA_2993. bat:BAS2782.
PATRIC	18783598. VBIBacAnt69550_2974.
TIGR	BA_2993. GBAA_2993.
Phylogenomic databases
GeneTree	EBGT00050000001887.
HOGENOM	HBG507643.
OMA	TFGDNDM.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	BANT260799:BAS2782-MONOMER. BANT261594:GBAA2993-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BACAN

Accession

Primary (citable) accession number: Q81P26
Secondary accession number(s): Q6HX98, Q6KRC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program