Reviewed, UniProtKB/Swiss-Prot Q81NJ6 (ARSC_BACAN)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:	arsC
Ordered Locus Names:	BA_3196, GBAA_3196, BAS2971

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Protein arsC HAMAP MF_01624		PRO_0000162511
Sites
	Active site	11	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	83	1	Nucleophile; for reductase activity By similarity
	Active site	90	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 83		Redox-active; alternate By similarity
	Disulfide bond	83 ↔ 90		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81NJ6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 73B208FC18C4683E	FASTA	134	15,066
	10 20 30 40 50 60 MENKKTIYFL CTGNSCRSQM AEAWGKQYLG DKWNVYSAGI EAHGVNPNAI KAMNEVNIDI 70 80 90 100 110 120 TNQTSDIIDA NILNRADLVV TLCSHADAVC PSTPPHVNRV HWGFDDPAGK EWPEFQRVRD 130 EIGERIKRFS ETGE

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP26992.1. AE017334 Genomic DNA. Translation: AAT32312.1. AE017225 Genomic DNA. Translation: AAT55279.1.
RefSeq	NP_845506.1. YP_019837.1. YP_029228.1.
3D structure databases
HSSP	HSSP built from PDB template 1JL3 based on UniProtKB P45947.
SMR	Q81NJ6. Positions 5-134.
ModBase	Search...
Genome annotation databases
GeneID	1086394. 2817664. 2850718.
GenomeReviews	Gene locus BA_3196 in contig AE016879_GR. Gene locus BAS2971 in contig AE017225_GR. Gene locus GBAA_3196 in contig AE017334_GR.
KEGG	ban:BA3196. bar:GBAA3196. bat:BAS2971.
TIGR	BA_3196. GBAA_3196.
Phylogenomic databases
HOGENOM	Q81NJ6.
OMA	EVNIDIT.
Enzyme and pathway databases
BioCyc	BANT260799:BAS2971-MON. BANT261594:GBAA3196-MON.
BRENDA	3.1.3.48. 267517.
Family and domain databases
HAMAP	MF_01624. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_BACAN

Accession

Primary (citable) accession number: Q81NJ6
Secondary accession number(s): Q6HWR0, Q6KQV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)