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Protein

1-deoxy-D-xylulose 5-phosphate reductoisomerase 1

Gene

dxr1

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase 1 (dxr1), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr2), 1-deoxy-D-xylulose 5-phosphate reductoisomerase 2 (dxr2), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr1), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ipk), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ipk), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231SubstrateUniRule annotation
Metal bindingi148 – 1481Divalent metal cationUniRule annotation
Metal bindingi150 – 1501Divalent metal cationUniRule annotation
Binding sitei150 – 1501SubstrateUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation
Binding sitei197 – 1971SubstrateUniRule annotation
Metal bindingi219 – 2191Divalent metal cationUniRule annotation
Binding sitei219 – 2191SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 3730NADPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

BioCyciANTHRA:DXR-1-MONOMER.
BANT260799:GJAJ-3222-MONOMER.
BANT261594:GJ7F-3332-MONOMER.
UniPathwayiUPA00056; UER00092.

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase 1UniRule annotation (EC:1.1.1.267UniRule annotation)
Short name:
DXP reductoisomerase 1UniRule annotation
Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase 1UniRule annotation
2-C-methyl-D-erythritol 4-phosphate synthase 1UniRule annotation
Gene namesi
Name:dxr1UniRule annotation
Synonyms:dxr-1
Ordered Locus Names:BA_3409, GBAA_3409, BAS3160
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3853851-deoxy-D-xylulose 5-phosphate reductoisomerase 1PRO_0000163600Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_3409.

Structurei

3D structure databases

ProteinModelPortaliQ81N10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DXR family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CEA. Bacteria.
COG0743. LUCA.
HOGENOMiHOG000007220.
KOiK00099.
OMAiFIDGSVM.
OrthoDBiEOG6R2H04.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81N10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKYISILGS TGSIGTSALD VVSAHPEHFK IVGLTANYNI ELLEQQIKTF
60 70 80 90 100
QPRIVSVATK ELADTLRTRI STNTKITYGT DGLIAVATHP NSNLVLSSVV
110 120 130 140 150
GVSGLLPTIE ALKAKKDIAI ANKETLVAAG HIVTELAKQN GCRLIPVDSE
160 170 180 190 200
HSAIFQCLNG ENNKEIDKLI VTASGGAFRD KTREEMKTLQ AKDALKHPNW
210 220 230 240 250
LMGAKLTIDS ATLMNKGFEV MEARWLFDIP YEKINVMIHK ESIIHSLVEF
260 270 280 290 300
IDGSVIAQLG APDMRMPIQY AFHYPTRLPS SYEKLNLLEI GSLHFEKPDL
310 320 330 340 350
EKFPCLQYAY ECGKIGGITP AVLNAANEIA NALFLKNEIA FFDIEKTIYK
360 370 380
TVEAHHNVKD PSLDAILEAD QWARQYANQL LIKKS
Length:385
Mass (Da):42,557
Last modified:June 1, 2003 - v1
Checksum:i4C9AA6CDAD4F368F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27179.1.
AE017334 Genomic DNA. Translation: AAT32518.1.
AE017225 Genomic DNA. Translation: AAT55468.1.
RefSeqiNP_845693.1. NC_003997.3.
WP_000241808.1. NZ_LHUO01000006.1.
YP_029417.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP27179; AAP27179; BA_3409.
AAT32518; AAT32518; GBAA_3409.
AAT55468; AAT55468; BAS3160.
GeneIDi1085180.
2849772.
KEGGiban:BA_3409.
bar:GBAA_3409.
bat:BAS3160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27179.1.
AE017334 Genomic DNA. Translation: AAT32518.1.
AE017225 Genomic DNA. Translation: AAT55468.1.
RefSeqiNP_845693.1. NC_003997.3.
WP_000241808.1. NZ_LHUO01000006.1.
YP_029417.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81N10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_3409.

Protocols and materials databases

DNASUi1085180.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP27179; AAP27179; BA_3409.
AAT32518; AAT32518; GBAA_3409.
AAT55468; AAT55468; BAS3160.
GeneIDi1085180.
2849772.
KEGGiban:BA_3409.
bar:GBAA_3409.
bat:BAS3160.

Phylogenomic databases

eggNOGiENOG4105CEA. Bacteria.
COG0743. LUCA.
HOGENOMiHOG000007220.
KOiK00099.
OMAiFIDGSVM.
OrthoDBiEOG6R2H04.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00092.
BioCyciANTHRA:DXR-1-MONOMER.
BANT260799:GJAJ-3222-MONOMER.
BANT261594:GJ7F-3332-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiDXR1_BACAN
AccessioniPrimary (citable) accession number: Q81N10
Secondary accession number(s): Q6HW71, Q6KQC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: January 20, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.