Q81MW4 (SPX2_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein spx 2

Gene names

Name:	spxA2
Ordered Locus Names:	BA_3456, GBAA_3456, BAS3202

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity. HAMAP MF_01132
Subcellular location	Cytoplasm Probable HAMAP MF_01132.
Sequence similarities	Belongs to the ArsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Regulatory protein spx 2 HAMAP MF_01132		PRO_0000162546
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81MW4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4727C97A296D9399	FASTA	131	15,325
	10 20 30 40 50 60 MVVLYTTASC ASCRKAKAWL EENQIDYTEK NIVSNSMTVD ELKSILRLTE EGATEIISTR 70 80 90 100 110 120 SKTFQDLNIN IEELSLNEFY KLIIEHPLML RRPIMLDEKR LQIGFNDEEI RKFLPRSVRT 130 FLNIELQKLA N

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP27225.1. AE017334 Genomic DNA. Translation: AAT32564.1. AE017225 Genomic DNA. Translation: AAT55510.1.
RefSeq	NP_845739.1. NC_003997.3. YP_020089.1. NC_007530.2. YP_029459.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81MW4.
SMR	Q81MW4. Positions 1-118.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000010692; EBBACP00000010448; EBBACG00000010684. EBBACT00000018391; EBBACP00000018012; EBBACG00000018382. EBBACT00000021201; EBBACP00000020686; EBBACG00000021192.
GeneID	1085665. 2814434. 2851241.
GenomeReviews	Gene locus BA_3456 in contig AE016879_GR. Gene locus BAS3202 in contig AE017225_GR. Gene locus GBAA_3456 in contig AE017334_GR.
KEGG	ban:BA_3456. bar:GBAA_3456. bat:BAS3202.
TIGR	BA_3456. GBAA_3456.
Phylogenomic databases
GeneTree	EBGT00050000001358.
HOGENOM	HBG734370.
OMA	YHEEDIR.
ProtClustDB	PRK12559.
Enzyme and pathway databases
BioCyc	BANT260799:BAS3202-MONOMER. BANT261594:GBAA3456-MONOMER.
Family and domain databases
HAMAP	MF_01132. SpxA. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR012336. Thioredoxin-like_fold. IPR023731. Tscrpt_reg_Spx. IPR006504. Tscrpt_reg_Spx/MgsR. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00537.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01617. ArsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX2_BACAN

Accession

Primary (citable) accession number: Q81MW4
Secondary accession number(s): Q6HW29, Q6KQ84

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program