Q81M54 (DXS_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BA_4400, GBAA_4400, BAS4081

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189083

Sequences

	Sequence		Length	Mass (Da)
	Q81M54 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 29320ADE58BB6B1D	FASTA	630	69,427
	10 20 30 40 50 60 MDLTQIQNPS FLKDMSISEL EGLSEDIRKF LIEELSQTGG HIAPNLGVVE LTIALHKLFD 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKIL TGRAKEFGTL RQYQGLCGFP KRCESEHDVW ETGHSSTSLS 130 140 150 160 170 180 AAMGMALARD LKKTKEYVIP IIGDGALTGG MALEALNHIG HEKTDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGALHNVLGR LRTAGKYHWV KDELEYILKK IPAVGGKVAA TAEKIKDSLK YLLVSGVFFE 250 260 270 280 290 300 ELGFTYLGPV DGHDYEKLFE TLQYAKKTKG PVLVHVITKK GKGYKPAESD VIGTWHGTGP 310 320 330 340 350 360 YKIESGDFVK PKEVAPAWSA VVSETVLKLA RTDERIVAIT PAMPVGSKLE KFQKEFPDRM 370 380 390 400 410 420 IDVGIAEQHA TTMAAGMATQ GMKPFLAIYS TFLQRAYDQV VHDICRQNLN VFIGIDRSGL 430 440 450 460 470 480 VGADGETHQG VFDISFLRHL PNMVIMMPKD ENEGQHLVYT AMQYEDGPIA LRYARGNGLG 490 500 510 520 530 540 VHMDEELKAI PIGSWETLKE GTQAAILTFG TTIPMAMEAA ERLEKAGVSV KVVNARFIKP 550 560 570 580 590 600 MDEAYLHDLL GKNIPILTIE EACLIGGFGT GVVEFASENG YHSALVERMG IPDRFIEHGS 610 620 630 VTKLLEEIGL TTDAVVDRIH TMIPSKQKRA

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28115.1. AE017334 Genomic DNA. Translation: AAT33519.1. AE017225 Genomic DNA. Translation: AAT56382.1.
RefSeq	NP_846629.1. NC_003997.3. YP_021044.1. NC_007530.2. YP_030331.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81M54.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000011092; EBBACP00000010848; EBBACG00000011084. EBBACT00000016687; EBBACP00000016308; EBBACG00000016678. EBBACT00000023815; EBBACP00000023300; EBBACG00000023806.
GeneID	1087729. 2819989. 2852469.
GenomeReviews	Gene locus BA_4400 in contig AE016879_GR. Gene locus BAS4081 in contig AE017225_GR. Gene locus GBAA_4400 in contig AE017334_GR.
KEGG	ban:BA_4400. bar:GBAA_4400. bat:BAS4081.
TIGR	BA_4400. GBAA_4400.
Phylogenomic databases
GeneTree	EBGT00070000031844.
HOGENOM	HBG571647.
OMA	RFANEHD.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4081-MONOMER. BANT261594:GBAA4400-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACAN

Accession

Primary (citable) accession number: Q81M54
Secondary accession number(s): Q6HTK7, Q6KMU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program