Q81M06 (GCST_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BA_4449, GBAA_4449, BAS4131

Organism

Bacillus anthracis [Reference proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP-Rule MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP-Rule MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Aminomethyltransferase HAMAP-Rule MF_00259		PRO_0000122535

Sequences

	Sequence		Length	Mass (Da)
	Q81M06 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B20A4A96ACF8D4BA	FASTA	366	40,225
	10 20 30 40 50 60 MITLQRTPLF DVYAKYGGKT IDFGGWELPV QFSSIKEEHE AVRTAAGLFD VSHMGEVEVK 70 80 90 100 110 120 GVDSLAFLQR VVTNDVSTLK VGGAQYTAMC YENGGTVDDL LIYKRGEEDY LLVINASNIE 130 140 150 160 170 180 KDYEWLASHV IGDATVVNVS SEVAQLAIQG PKAEGILQKV VSEDLKEIKF FKFKNDILVD 190 200 210 220 230 240 GIPALVSRTG YTGEDGFEIY CKSEDAAKLW EKLLEVGAEE GLKACGLGAR DTLRFEATLP 250 260 270 280 290 300 LYGQELSKDI TPIEAGIGFA VKTNKEADFF GKATLKEQKE NGAPRKLVGI EVIERGIPRT 310 320 330 340 350 360 HYPVFIGEEK IGEVTSGTQS PTLKKSIGLA LIDVKYAAVD TEVEIEIRNK RVKAVVVPTP FYKRSK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'." Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A. J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28163.1. AE017334 Genomic DNA. Translation: AAT33568.1. AE017225 Genomic DNA. Translation: AAT56431.1.
RefSeq	NP_846677.1. NC_003997.3. YP_021093.1. NC_007530.2. YP_030380.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81M06.
SMR	Q81M06. Positions 4-364.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81M06. 2 interactions.
STRING	198094.BA_4449.
Protocols and materials databases
DNASU	1087845.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP28163; AAP28163; BA_4449. AAT33568; AAT33568; GBAA_4449. AAT56431; AAT56431; BAS4131.
GeneID	1087845. 2818765. 2849651.
KEGG	ban:BA_4449. bar:GBAA_4449. bat:BAS4131.
Phylogenomic databases
eggNOG	COG0404.
HOGENOM	HOG000239381.
KO	K00605.
OMA	LGARDVC.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	BANT260799:GJAJ-4187-MONOMER. BANT261594:GJ7F-4328-MONOMER.
Family and domain databases
Gene3D	3.30.1360.120. 2 hits.
HAMAP	MF_00259. GcvT.
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. IPR027266. TrmE/GcvT_dom1. [Graphical view]
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BACAN

Accession

Primary (citable) accession number: Q81M06
Secondary accession number(s): Q6HTF8, Q6KMQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program