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Protein

Probable endonuclease 4

Gene

nfo

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1UniRule annotation1
Metal bindingi111Zinc 1UniRule annotation1
Metal bindingi146Zinc 1UniRule annotation1
Metal bindingi146Zinc 2UniRule annotation1
Metal bindingi180Zinc 2UniRule annotation1
Metal bindingi183Zinc 3UniRule annotation1
Metal bindingi215Zinc 2UniRule annotation1
Metal bindingi228Zinc 3UniRule annotation1
Metal bindingi230Zinc 3UniRule annotation1
Metal bindingi260Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciANTHRA:NFO-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:BA_4508, GBAA_4508, BAS4186
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908191 – 298Probable endonuclease 4Add BLAST298

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_4508.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi12 – 14Combined sources3
Helixi16 – 24Combined sources9
Turni25 – 27Combined sources3
Beta strandi29 – 33Combined sources5
Helixi46 – 49Combined sources4
Helixi51 – 60Combined sources10
Beta strandi66 – 69Combined sources4
Helixi82 – 101Combined sources20
Beta strandi102 – 104Combined sources3
Beta strandi107 – 110Combined sources4
Helixi120 – 134Combined sources15
Beta strandi140 – 146Combined sources7
Helixi159 – 168Combined sources10
Helixi172 – 174Combined sources3
Beta strandi175 – 180Combined sources6
Helixi181 – 186Combined sources6
Helixi191 – 205Combined sources15
Helixi208 – 210Combined sources3
Beta strandi211 – 216Combined sources6
Beta strandi218 – 221Combined sources4
Beta strandi235 – 238Combined sources4
Helixi240 – 247Combined sources8
Helixi250 – 252Combined sources3
Beta strandi257 – 259Combined sources3
Beta strandi267 – 269Combined sources3
Helixi275 – 284Combined sources10
Helixi291 – 296Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XP3X-ray2.57A1-298[»]
ProteinModelPortaliQ81LV1.
SMRiQ81LV1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81LV1.

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81LV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIGSHVSM SGKKMLLAAS EEAVSYGATT FMIYTGAPQN TRRKPIEELN
60 70 80 90 100
IEAGRKHMEQ NGIEEIIVHA PYIINVGNTT KPETFQLGVD FLRMEIERTS
110 120 130 140 150
ALGVAKQIVL HPGAHVGAGA DAGIQQIIKG LNEVLTPDQT VNIALETMAG
160 170 180 190 200
KGTECGRSFE EIAKIIDGVK YNEKLSVCFD TCHTHDAGYD IVNNFDGVLN
210 220 230 240 250
EFDKIVGIDR LQVLHINDSK NVRGAGKDRH ENIGFGHIGY KALHHIVHHP
260 270 280 290
QLTHVPKILE TPYVGEDKKD KKPPYKLEIE MLKNGTFDEG LLEKIKAQ
Length:298
Mass (Da):32,909
Last modified:June 1, 2003 - v1
Checksum:iC5178AB085784C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28219.1.
AE017334 Genomic DNA. Translation: AAT33627.1.
AE017225 Genomic DNA. Translation: AAT56486.1.
RefSeqiNP_846733.1. NC_003997.3.
WP_000912468.1. NZ_LHUO01000006.1.
YP_030435.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP28219; AAP28219; BA_4508.
AAT33627; AAT33627; GBAA_4508.
AAT56486; AAT56486; BAS4186.
GeneIDi1088232.
2852108.
KEGGiban:BA_4508.
bar:GBAA_4508.
bat:BAS4186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28219.1.
AE017334 Genomic DNA. Translation: AAT33627.1.
AE017225 Genomic DNA. Translation: AAT56486.1.
RefSeqiNP_846733.1. NC_003997.3.
WP_000912468.1. NZ_LHUO01000006.1.
YP_030435.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XP3X-ray2.57A1-298[»]
ProteinModelPortaliQ81LV1.
SMRiQ81LV1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_4508.

Protocols and materials databases

DNASUi1088232.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP28219; AAP28219; BA_4508.
AAT33627; AAT33627; GBAA_4508.
AAT56486; AAT56486; BAS4186.
GeneIDi1088232.
2852108.
KEGGiban:BA_4508.
bar:GBAA_4508.
bat:BAS4186.

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.

Enzyme and pathway databases

BioCyciANTHRA:NFO-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81LV1.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEND4_BACAN
AccessioniPrimary (citable) accession number: Q81LV1
Secondary accession number(s): Q6HTA3, Q6KMJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.