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Protein

Probable endonuclease 4

Gene

nfo

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1UniRule annotation
Metal bindingi111 – 1111Zinc 1UniRule annotation
Metal bindingi146 – 1461Zinc 1UniRule annotation
Metal bindingi146 – 1461Zinc 2UniRule annotation
Metal bindingi180 – 1801Zinc 2UniRule annotation
Metal bindingi183 – 1831Zinc 3UniRule annotation
Metal bindingi215 – 2151Zinc 2UniRule annotation
Metal bindingi228 – 2281Zinc 3UniRule annotation
Metal bindingi230 – 2301Zinc 3UniRule annotation
Metal bindingi260 – 2601Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciANTHRA:NFO-MONOMER.
BANT260799:GJAJ-4243-MONOMER.
BANT261594:GJ7F-4385-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:BA_4508, GBAA_4508, BAS4186
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable endonuclease 4PRO_0000190819Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_4508.

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Beta strandi12 – 143Combined sources
Helixi16 – 249Combined sources
Turni25 – 273Combined sources
Beta strandi29 – 335Combined sources
Helixi46 – 494Combined sources
Helixi51 – 6010Combined sources
Beta strandi66 – 694Combined sources
Helixi82 – 10120Combined sources
Beta strandi102 – 1043Combined sources
Beta strandi107 – 1104Combined sources
Helixi120 – 13415Combined sources
Beta strandi140 – 1467Combined sources
Helixi159 – 16810Combined sources
Helixi172 – 1743Combined sources
Beta strandi175 – 1806Combined sources
Helixi181 – 1866Combined sources
Helixi191 – 20515Combined sources
Helixi208 – 2103Combined sources
Beta strandi211 – 2166Combined sources
Beta strandi218 – 2214Combined sources
Beta strandi235 – 2384Combined sources
Helixi240 – 2478Combined sources
Helixi250 – 2523Combined sources
Beta strandi257 – 2593Combined sources
Beta strandi267 – 2693Combined sources
Helixi275 – 28410Combined sources
Helixi291 – 2966Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XP3X-ray2.57A1-298[»]
ProteinModelPortaliQ81LV1.
SMRiQ81LV1. Positions 2-298.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81LV1.

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81LV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIGSHVSM SGKKMLLAAS EEAVSYGATT FMIYTGAPQN TRRKPIEELN
60 70 80 90 100
IEAGRKHMEQ NGIEEIIVHA PYIINVGNTT KPETFQLGVD FLRMEIERTS
110 120 130 140 150
ALGVAKQIVL HPGAHVGAGA DAGIQQIIKG LNEVLTPDQT VNIALETMAG
160 170 180 190 200
KGTECGRSFE EIAKIIDGVK YNEKLSVCFD TCHTHDAGYD IVNNFDGVLN
210 220 230 240 250
EFDKIVGIDR LQVLHINDSK NVRGAGKDRH ENIGFGHIGY KALHHIVHHP
260 270 280 290
QLTHVPKILE TPYVGEDKKD KKPPYKLEIE MLKNGTFDEG LLEKIKAQ
Length:298
Mass (Da):32,909
Last modified:June 1, 2003 - v1
Checksum:iC5178AB085784C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28219.1.
AE017334 Genomic DNA. Translation: AAT33627.1.
AE017225 Genomic DNA. Translation: AAT56486.1.
RefSeqiNP_846733.1. NC_003997.3.
WP_000912468.1. NZ_LHUO01000006.1.
YP_030435.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP28219; AAP28219; BA_4508.
AAT33627; AAT33627; GBAA_4508.
AAT56486; AAT56486; BAS4186.
GeneIDi1088232.
2852108.
KEGGiban:BA_4508.
bar:GBAA_4508.
bat:BAS4186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28219.1.
AE017334 Genomic DNA. Translation: AAT33627.1.
AE017225 Genomic DNA. Translation: AAT56486.1.
RefSeqiNP_846733.1. NC_003997.3.
WP_000912468.1. NZ_LHUO01000006.1.
YP_030435.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XP3X-ray2.57A1-298[»]
ProteinModelPortaliQ81LV1.
SMRiQ81LV1. Positions 2-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_4508.

Protocols and materials databases

DNASUi1088232.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP28219; AAP28219; BA_4508.
AAT33627; AAT33627; GBAA_4508.
AAT56486; AAT56486; BAS4186.
GeneIDi1088232.
2852108.
KEGGiban:BA_4508.
bar:GBAA_4508.
bat:BAS4186.

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Enzyme and pathway databases

BioCyciANTHRA:NFO-MONOMER.
BANT260799:GJAJ-4243-MONOMER.
BANT261594:GJ7F-4385-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81LV1.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiEND4_BACAN
AccessioniPrimary (citable) accession number: Q81LV1
Secondary accession number(s): Q6HTA3, Q6KMJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: January 20, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.