Q81LH2 (TGT_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	BA_4647, GBAA_4647, BAS4312

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135444
Sites
	Active site	94	1	Nucleophile By similarity
	Metal binding	306	1	Zinc By similarity
	Metal binding	308	1	Zinc By similarity
	Metal binding	311	1	Zinc By similarity
	Metal binding	337	1	Zinc By similarity
	Binding site	95	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81LH2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6DE1E29B88568644	FASTA	379	43,227
	10 20 30 40 50 60 MTAIRYEFIK TCKQTGARLG RVHTPHGSFD TPTFMPVGTL ATVKTMSPEE LKAMDSGIIL 70 80 90 100 110 120 SNTYHLWLRP GHEIIREAGG LHKFMNWDRA ILTDSGGFQV FSLSDFRRIE EEGVHFRNHL 130 140 150 160 170 180 NGDKLFLSPE KAMEIQNALG SDIMMAFDEC PPFPATFEYM KKSVERTSRW AERCLKAHER 190 200 210 220 230 240 PQDQGLFGIV QGGEFEELRR QSAKDLVSMD FPGYAVGGLS VGEPKDIMNR VLEFTTPLLP 250 260 270 280 290 300 DNKPRYLMGV GSPDSLIDGA IRGIDMFDCV LPTRIARNGT CMTSEGRLVV KNAKFARDFG 310 320 330 340 350 360 PLDPNCDCYT CKNYSRAYIR HLMKCDETFG IRLTSYHNLH FLLNLMEQVR QAIREDRLGD 370 FREEFFEQYG FNKPNAKNF

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28350.1. AE017334 Genomic DNA. Translation: AAT33769.1. AE017225 Genomic DNA. Translation: AAT56610.1.
RefSeq	NP_846864.1. NC_003997.3. YP_021294.1. NC_007530.2. YP_030559.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81LH2.
SMR	Q81LH2. Positions 5-369.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000011678; EBBACP00000011434; EBBACG00000011670. EBBACT00000017115; EBBACP00000016736; EBBACG00000017106. EBBACT00000024128; EBBACP00000023613; EBBACG00000024119.
GeneID	1084870. 2819339. 2852840.
GenomeReviews	Gene locus BA_4647 in contig AE016879_GR. Gene locus BAS4312 in contig AE017225_GR. Gene locus GBAA_4647 in contig AE017334_GR.
KEGG	ban:BA_4647. bar:GBAA_4647. bat:BAS4312.
TIGR	BA_4647. GBAA_4647.
Phylogenomic databases
GeneTree	EBGT00050000001431.
HOGENOM	HBG629929.
OMA	FMPVGTV.
ProtClustDB	PRK00112.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4312-MONOMER. BANT261594:GBAA4647-MONOMER.
Family and domain databases
HAMAP	MF_00168. Q_tRNA_Tgt. [Tree]
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KO	K00773.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. Tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BACAN

Accession

Primary (citable) accession number: Q81LH2
Secondary accession number(s): Q6HSX9, Q6KM67

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program