Q81LB0 (NTPA_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BA_4714, GBAA_4714, BAS4376

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.
Sequence caution	The sequence AAT56674.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178119
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	41	1	Magnesium or manganese By similarity
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81LB0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5E54C0BE4642395D	FASTA	202	22,849
	10 20 30 40 50 60 MKQVVVATKN MGKVREFAEL FERFDLEVKS LHDFPHIEEV EETGETFEEN AILKADSLSR 70 80 90 100 110 120 QLNAIVIADD SGLIVDALNG KPGVYSARFA GEPKDDQANI DKVLQELNEV AFEKRKARFY 130 140 150 160 170 180 CALAVAFPEG DKKPVIVNGT CEGFILEQRR GENGFGYDPI FYVEEYKKAM AELSSDEKNA 190 200 ISHRGRALRK LEEKIPEWFL GE

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28412.1. AE017334 Genomic DNA. Translation: AAT33838.1. AE017225 Genomic DNA. Translation: AAT56674.1. Different initiation.
RefSeq	NP_846926.1. NC_003997.3. YP_021363.1. NC_007530.2. YP_030623.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81LB0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81LB0. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000012251; EBBACP00000012007; EBBACG00000012243. EBBACT00000013968; EBBACP00000013589; EBBACG00000013960. EBBACT00000023442; EBBACP00000022927; EBBACG00000023433.
GeneID	1083679. 2815980. 2851171.
GenomeReviews	Gene locus BA_4714 in contig AE016879_GR. Gene locus BAS4376 in contig AE017225_GR. Gene locus GBAA_4714 in contig AE017334_GR.
KEGG	ban:BA_4714. bar:GBAA_4714. bat:BAS4376.
TIGR	BA_4714. GBAA_4714.
Phylogenomic databases
GeneTree	EBGT00050000001334.
HOGENOM	HBG697237.
OMA	GEAHNDA.
ProtClustDB	PRK14822.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4376-MONOMER. BANT261594:GBAA4714-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BACAN

Accession

Primary (citable) accession number: Q81LB0
Secondary accession number(s): Q6HSR5, Q6KM06

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program