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Protein

dITP/XTP pyrophosphatase

Gene

BA_4714

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41MagnesiumUniRule annotation1
Active sitei70Proton acceptorUniRule annotation1
Metal bindingi70MagnesiumUniRule annotation1
Binding sitei71Substrate; via amide nitrogenUniRule annotation1
Binding sitei178SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:BA_4714, GBAA_4714, BAS4376
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001781191 – 202dITP/XTP pyrophosphataseAdd BLAST202

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ81LB0. 1 interactor.
STRINGi260799.BAS4376.

Structurei

3D structure databases

ProteinModelPortaliQ81LB0.
SMRiQ81LB0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 13Substrate bindingUniRule annotation6
Regioni155 – 158Substrate bindingUniRule annotation4
Regioni183 – 184Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81LB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQVVVATKN MGKVREFAEL FERFDLEVKS LHDFPHIEEV EETGETFEEN
60 70 80 90 100
AILKADSLSR QLNAIVIADD SGLIVDALNG KPGVYSARFA GEPKDDQANI
110 120 130 140 150
DKVLQELNEV AFEKRKARFY CALAVAFPEG DKKPVIVNGT CEGFILEQRR
160 170 180 190 200
GENGFGYDPI FYVEEYKKAM AELSSDEKNA ISHRGRALRK LEEKIPEWFL

GE
Length:202
Mass (Da):22,849
Last modified:June 1, 2003 - v1
Checksum:i5E54C0BE4642395D
GO

Sequence cautioni

The sequence AAT56674 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28412.1.
AE017334 Genomic DNA. Translation: AAT33838.1.
AE017225 Genomic DNA. Translation: AAT56674.1. Different initiation.
RefSeqiNP_846926.1. NC_003997.3.

Genome annotation databases

EnsemblBacteriaiAAP28412; AAP28412; BA_4714.
AAT33838; AAT33838; GBAA_4714.
AAT56674; AAT56674; BAS4376.
GeneIDi1083679.
KEGGiban:BA_4714.
bar:GBAA_4714.
PATRICifig|198094.11.peg.4679.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIXTPA_BACAN
AccessioniPrimary (citable) accession number: Q81LB0
Secondary accession number(s): Q6HSR5, Q6KM06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: July 5, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families