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Protein

Ribonuclease PH

Gene

rph

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Miscellaneous

Sulfate ions in the crystal structure may represent the inorganic phosphate substrate.1 Publication

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Phosphate (substrate) bindingUniRule annotation1 Publication1

GO - Molecular functioni

  • ribonuclease activity Source: TIGR
  • tRNA binding Source: UniProtKB-KW
  • tRNA nucleotidyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  • rRNA processing Source: UniProtKB-KW
  • tRNA processing Source: TIGR

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:BA_4715, GBAA_4715, BAS4377
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001398621 – 245Ribonuclease PHAdd BLAST245

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers (PubMed:19153445).UniRule annotation1 Publication

Protein-protein interaction databases

IntActiQ81LA9, 1 interactor
STRINGi260799.BAS4377

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 19Combined sources6
Beta strandi21 – 32Combined sources12
Beta strandi35 – 45Combined sources11
Helixi49 – 51Combined sources3
Beta strandi58 – 65Combined sources8
Beta strandi69 – 72Combined sources4
Helixi85 – 101Combined sources17
Helixi104 – 107Combined sources4
Beta strandi111 – 120Combined sources10
Helixi125 – 146Combined sources22
Beta strandi149 – 152Combined sources4
Beta strandi155 – 157Combined sources3
Beta strandi159 – 167Combined sources9
Turni168 – 170Combined sources3
Beta strandi171 – 175Combined sources5
Helixi178 – 182Combined sources5
Beta strandi184 – 193Combined sources10
Beta strandi198 – 208Combined sources11
Helixi212 – 237Combined sources26
Helixi238 – 243Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DD6X-ray1.70A1-245[»]
ProteinModelPortaliQ81LA9
SMRiQ81LA9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81LA9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 126Phosphate (substrate) bindingUniRule annotation1 Publication3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108FK2 Bacteria
COG0689 LUCA
HOGENOMiHOG000229516
KOiK00989
OMAiKGKGQGW

Family and domain databases

CDDicd11362 RNase_PH_bact, 1 hit
Gene3Di3.30.230.70, 1 hit
HAMAPiMF_00564 RNase_PH, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR002381 RNase_PH_bac-type
IPR018336 RNase_PH_CS
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55666 SSF55666, 1 hit
TIGRFAMsiTIGR01966 RNasePH, 1 hit
PROSITEiView protein in PROSITE
PS01277 RIBONUCLEASE_PH, 1 hit

Sequencei

Sequence statusi: Complete.

Q81LA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVDGREKTE LRHIHIHTNY LKHPEGSVLI EVGDTKVICS ATIEERVPPF
60 70 80 90 100
MRGEGKGWVT AEYAMIPRAT EQRTIRESSK GKVTGRTMEI QRLIGRALRA
110 120 130 140 150
VVDLEALGER TVWIDCDVIQ ADGGTRTASI TGAYVAMVLA FEKLLQAEKV
160 170 180 190 200
SKIPVKDYLA ATSVGIVEEQ GVVLDLNYAE DSKADVDMNV IMTGKGQFVE
210 220 230 240
VQGTGEEATF SRAQLNELLD AAEQGIFQLI DIQKEALGDI VSHIE
Length:245
Mass (Da):26,983
Last modified:June 1, 2003 - v1
Checksum:i00053DA2C582D0E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP28413.1
AE017334 Genomic DNA Translation: AAT33839.1
AE017225 Genomic DNA Translation: AAT56675.1
RefSeqiNP_846927.1, NC_003997.3
WP_001261764.1, NZ_PPES01000001.1
YP_030624.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP28413; AAP28413; BA_4715
AAT33839; AAT33839; GBAA_4715
AAT56675; AAT56675; BAS4377
GeneIDi1088544
2851699
KEGGiban:BA_4715
bar:GBAA_4715
bat:BAS4377
PATRICifig|198094.11.peg.4680

Similar proteinsi

Entry informationi

Entry nameiRNPH_BACAN
AccessioniPrimary (citable) accession number: Q81LA9
Secondary accession number(s): Q6HSR4, Q6KM05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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