Reviewed,
UniProtKB/Swiss-Prot Q81L50 (ISDG_BACAN)
Last modified
June 16, 2009.
Version 47.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Heme-degrading monooxygenase isdG EC=1.14.99.3 Alternative name(s): Iron-regulated surface determinant isdG Iron-responsive surface determinant isdG Heme oxygenase | ||||
| Gene names |
| ||||
| Organism | Bacillus anthracis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 1392 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group |
Protein attributes
| Sequence length | 107 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron. Is also necessary to protect B.anthracis from heme-mediated toxicity that might be encountered upon exposure to oxidative radicals. Ref.4 |
| Catalytic activity | Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. Ref.4 |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Induction | Up-regulated under iron-starved conditions Probable. |
| Sequence similarities | Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | Heme Iron Metal-binding |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | antibiotic biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activityInferred from electronic annotation. Source: EC monooxygenase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 107 | 107 | Heme-degrading monooxygenase isdG HAMAP MF_01272 | PRO_0000270070 | |||||
Sites | |||||||||
| Metal binding | 6 | 1 | Iron Potential | ||||||
| Metal binding | 76 | 1 | Iron (heme axial ligand) Potential | ||||||
| Site | 66 | 1 | Transition state stabilizer Potential | ||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. Fraser C.M.Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton. |
| [2] | "Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne. |
| [3] | "Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor. |
| [4] | "Bacillus anthracis IsdG, a heme-degrading monooxygenase." Skaar E.P., Gaspar A.H., Schneewind O. J. Bacteriol. 188:1071-1080(2006) [PubMed: 16428411] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION. |
Cross-references
Sequence databases | |
|---|---|
| AE016879 Genomic DNA. Translation: AAP28473.1. AE017225 Genomic DNA. Translation: AAT56735.1. AE017334 Genomic DNA. Translation: AAT33903.1. | |
| RefSeq | NP_846987.1. YP_021428.1. YP_030684.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1083895. 2815699. 2851618. |
| GenomeReviews | Gene locus BA_4782 in contig AE016879_GR. Gene locus BAS4437 in contig AE017225_GR. Gene locus GBAA4782 in contig AE017334_GR. |
| KEGG | ban:BA4782. bar:GBAA4782. bat:BAS4437. |
| TIGR | BA_4782. GBAA_4782. |
Phylogenomic databases | |
| HOGENOM | Q81L50. |
| OMA | Q81L50. DAHSHQG. |
Enzyme and pathway databases | |
| BioCyc | BANT260799:BAS4437-MON. BANT261594:GBAA4782-MON. |
| BRENDA | 1.14.99.3. 267517. |
Family and domain databases | |
| HAMAP | MF_01272. [Tree] |
| InterPro | IPR007138. Antibiotic_mOase. [Graphical view] |
| Pfam | PF03992. ABM. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ISDG_BACAN | ||||||||
| Accession | Primary (citable) accession number: Q81L50 Secondary accession number(s): Q6HSK4, Q6KLU7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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