Reviewed, UniProtKB/Swiss-Prot Q81L32 (SYN_BACAN)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BA_4802, GBAA_4802, BAS4454

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176385

Sequences

	Sequence		Length	Mass (Da)
	Q81L32-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FE5150D1F046E044	FASTA	463	52,926
	10 20 30 40 50 60 MENTLVKSLY RDTDKYAGQT VQVSGWIRNL RDSKAFGFIE LNDGSFFKSV QIVFDTELDN 70 80 90 100 110 120 FKEIAKLPLS SSVKVEGKVI ATPGAKQPFE IKAEKIDIEG LSDSDYPLQK KRHTFEYLRT 130 140 150 160 170 180 IAHLRPRTNA FSATFRVRSI AAFAIHQFFQ ERGFVHVHTP IITGSDTEGA GEMFRVTTQD 190 200 210 220 230 240 LNNVPKGEDG QVDESKDFFG KETNLTVSGQ LNAEAYALAF RDVYTFGPTF RAENSNTTRH 250 260 270 280 290 300 AAEFWMVEPE IAFAELGDVM NLTEDMLKYA MKYVLEHAPE EMEFFNSFVD KTVLERMNNV 310 320 330 340 350 360 INSDFGRITY TEAIKVLQES GADFKYPVEW GIDLQTEHER YLSEEIFKRP VFVTDYPKDI 370 380 390 400 410 420 KAFYMRLNDD GKTVAATDLL VPGIGELIGG SQREERMDVL VDRIKELGMN EEDYWWYLEL 430 440 450 460 RKYGGTKHAG FGLGFERFLM YITGMANIRD VIPFPRTPGS SEF

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28491.1. AE017334 Genomic DNA. Translation: AAT33922.1. AE017225 Genomic DNA. Translation: AAT56752.1.
RefSeq	NP_847005.1. YP_021447.1. YP_030701.1.
3D structure databases
SMR	Q81L32. Positions 14-461.
ModBase	Search...
Genome annotation databases
GeneID	1083928. 2815787. 2851600.
GenomeReviews	Gene locus BA_4802 in contig AE016879_GR. Gene locus BAS4454 in contig AE017225_GR. Gene locus GBAA_4802 in contig AE017334_GR.
KEGG	ban:BA4802. bar:GBAA4802. bat:BAS4454.
TIGR	BA_4802. GBAA_4802.
Phylogenomic databases
HOGENOM	HBG745843.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4454-MONOMER. BANT261594:GBAA4802-MONOMER.
BRENDA	6.1.1.22. 267517.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACAN

Accession

Primary (citable) accession number: Q81L32
Secondary accession number(s): Q6HSI7, Q6KLT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)