Reviewed, UniProtKB/Swiss-Prot Q81KV8 (ARLY_BACAN)

Names and origin

Protein names

Recommended name:
    Argininosuccinate lyase
      Short name=ASAL
    EC=4.3.2.1
Alternative name(s):
    Arginosuccinase

Gene names

Name:	argH-2
Ordered Locus Names:	BA_4879, GBAA_4879, BAS4527

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Argininosuccinate lyase HAMAP MF_00006		PRO_0000137734

Sequences

	Sequence		Length	Mass (Da)
	Q81KV8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2BBACFDA26EBB4DA	FASTA	462	52,165
	10 20 30 40 50 60 MSKLWGGRFT EEAEAWVEEF GASISFDQQL VNQDINGSIA HVTMLAKQGI VTKEEAEKIK 70 80 90 100 110 120 IGLQYLLEEA KQNKLHFSVE AEDIHLNIEK MLMEKIGEVG GKLHTGRSRN DQVATDMHLY 130 140 150 160 170 180 LKEKVEHIIK AIKQLQTVLV HQAENNIETI MPGYTHLQRA QPISFAHHIL AYFWMLERDV 190 200 210 220 230 240 NRYEDSLKRI NISPLGAGAL AGTTFPIDRE YSAELLGFNG IYENSLDAVS DRDFILEFLS 250 260 270 280 290 300 NSSMLMMHLS RFCEELILWS SQEFQFIEMS DQYATGSSIM PQKKNPDMAE LIRGKTGRVY 310 320 330 340 350 360 GNLFSLLTVM KGLPLAYNKD LQEDKEGMFD TVKTVEGCLH IMAGMLETMT VNKEKMGQAV 370 380 390 400 410 420 TQDFSNATEI ADYLANKGLP FRQAHEIVGK LVLHCTQKGI YLIDVPLATY KEMSALFEED 430 440 450 460 LYEVLSPYAA VKRRNSAGGT GFEQIEKALE KAKGLTKEAI KN

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP28566.1. AE017334 Genomic DNA. Translation: AAT33996.1. AE017225 Genomic DNA. Translation: AAT56824.1.
RefSeq	NP_847080.1. YP_021521.1. YP_030774.1.
3D structure databases
HSSP	HSSP built from PDB template 1DCN based on UniProtKB P24058.
ModBase	Search...
Genome annotation databases
GeneID	1085916. 2816705. 2850359.
GenomeReviews	Gene locus BA_4879 in contig AE016879_GR. Gene locus BAS4527 in contig AE017225_GR. Gene locus GBAA_4879 in contig AE017334_GR.
KEGG	ban:BA4879. bar:GBAA4879. bat:BAS4527.
TIGR	BA_4879. GBAA_4879.
Phylogenomic databases
HOGENOM	Q81KV8.
OMA	MAEDLIF.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4527-MON. BANT261594:GBAA4879-MON.
BRENDA	4.3.2.1. 267517.
Family and domain databases
HAMAP	MF_00006. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00838. argH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY_BACAN

Accession

Primary (citable) accession number: Q81KV8
Secondary accession number(s): Q6HSB4, Q6KLL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)