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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS (By similarity).By similarity

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651ATP
Binding sitei287 – 2871ATP; via amide nitrogen
Binding sitei296 – 2961ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi183 – 1842ATP
Nucleotide bindingi208 – 2092ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciANTHRA:THII-MONOMER.
BANT260799:GJAJ-4602-MONOMER.
BANT261594:GJ7F-4758-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferase (EC:2.8.1.4)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene namesi
Name:thiI
Ordered Locus Names:BA_4899, GBAA_4899, BAS4545
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Probable tRNA sulfurtransferasePRO_0000154830Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_4899.

Structurei

Secondary structure

1
403
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Helixi18 – 3417Combined sources
Turni35 – 373Combined sources
Beta strandi42 – 454Combined sources
Beta strandi50 – 534Combined sources
Helixi59 – 668Combined sources
Beta strandi72 – 8413Combined sources
Helixi85 – 9713Combined sources
Beta strandi105 – 1128Combined sources
Helixi121 – 13313Combined sources
Beta strandi136 – 1383Combined sources
Beta strandi143 – 1453Combined sources
Beta strandi147 – 1548Combined sources
Beta strandi156 – 16712Combined sources
Turni174 – 1774Combined sources
Beta strandi179 – 1835Combined sources
Beta strandi186 – 1883Combined sources
Helixi189 – 19911Combined sources
Beta strandi203 – 2108Combined sources
Turni212 – 2143Combined sources
Helixi217 – 23014Combined sources
Helixi231 – 2333Combined sources
Beta strandi237 – 2437Combined sources
Helixi245 – 25410Combined sources
Helixi257 – 2593Combined sources
Helixi260 – 27819Combined sources
Beta strandi283 – 2853Combined sources
Beta strandi290 – 2923Combined sources
Helixi298 – 3047Combined sources
Helixi305 – 3073Combined sources
Turni315 – 3184Combined sources
Helixi321 – 33010Combined sources
Helixi334 – 3374Combined sources
Helixi359 – 3668Combined sources
Helixi372 – 38110Combined sources
Beta strandi383 – 3875Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C5SX-ray2.50A1-403[»]
ProteinModelPortaliQ81KU0.
SMRiQ81KU0. Positions 3-391.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81KU0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 165106THUMPAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.Curated
Contains 1 THUMP domain.Curated

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
HOGENOMiHOG000227470.
KOiK03151.
OMAiEAAYISH.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81KU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYEYILVRY GEMTTKGKNR SKFVSTLKDN VKFKLKKFPN IKIDATHDRM
60 70 80 90 100
YIQLNGEDHE AVSERLKDVF GIHKFNLAMK VPSELEDIKK GALAAFLQVK
110 120 130 140 150
GDVKTFKITV HRSYKHFPMR TMELLPEIGG HILENTEDIT VDVHNPDVNV
160 170 180 190 200
RVEIRSGYSY IMCDERMGAG GLPVGVGGKV MVLLSGGIDS PVAAYLTMKR
210 220 230 240 250
GVSVEAVHFH SPPFTSERAK QKVIDLAQEL TKYCKRVTLH LVPFTEVQKT
260 270 280 290 300
INKEIPSSYS MTVMRRMMMR ITERIAEERN ALAITTGESL GQVASQTLDS
310 320 330 340 350
MHTINEVTNY PVIRPLITMD KLEIIKIAEE IGTYDISIRP YEDCCTVFTP
360 370 380 390 400
ASPATKPKRE KANRFEAKYD FTPLIDEAVA NKETMVLQTV EVVAEEEKFE

ELF
Length:403
Mass (Da):45,711
Last modified:June 1, 2003 - v1
Checksum:iA87E445661268FA6
GO

Sequence cautioni

The sequence AAT56842 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28584.1.
AE017334 Genomic DNA. Translation: AAT34017.2.
AE017225 Genomic DNA. Translation: AAT56842.1. Different initiation.
RefSeqiNP_847098.1. NC_003997.3.
YP_030792.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP28584; AAP28584; BA_4899.
AAT34017; AAT34017; GBAA_4899.
AAT56842; AAT56842; BAS4545.
GeneIDi1087273.
2850079.
KEGGiban:BA_4899.
bar:GBAA_4899.
bat:BAS4545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28584.1.
AE017334 Genomic DNA. Translation: AAT34017.2.
AE017225 Genomic DNA. Translation: AAT56842.1. Different initiation.
RefSeqiNP_847098.1. NC_003997.3.
YP_030792.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C5SX-ray2.50A1-403[»]
ProteinModelPortaliQ81KU0.
SMRiQ81KU0. Positions 3-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_4899.

Protocols and materials databases

DNASUi1087273.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP28584; AAP28584; BA_4899.
AAT34017; AAT34017; GBAA_4899.
AAT56842; AAT56842; BAS4545.
GeneIDi1087273.
2850079.
KEGGiban:BA_4899.
bar:GBAA_4899.
bat:BAS4545.

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
HOGENOMiHOG000227470.
KOiK03151.
OMAiEAAYISH.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciANTHRA:THII-MONOMER.
BANT260799:GJAJ-4602-MONOMER.
BANT261594:GJ7F-4758-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81KU0.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHII_BACAN
AccessioniPrimary (citable) accession number: Q81KU0
Secondary accession number(s): Q6HS96, Q6KLJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.