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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS (By similarity).By similarity

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265ATP1
Binding sitei287ATP; via amide nitrogen1
Binding sitei296ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 184ATP2
Nucleotide bindingi208 – 209ATP2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciANTHRA:THII-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferase (EC:2.8.1.4)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene namesi
Name:thiI
Ordered Locus Names:BA_4899, GBAA_4899, BAS4545
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548301 – 403Probable tRNA sulfurtransferaseAdd BLAST403

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_4899.

Structurei

Secondary structure

1403
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi18 – 34Combined sources17
Turni35 – 37Combined sources3
Beta strandi42 – 45Combined sources4
Beta strandi50 – 53Combined sources4
Helixi59 – 66Combined sources8
Beta strandi72 – 84Combined sources13
Helixi85 – 97Combined sources13
Beta strandi105 – 112Combined sources8
Helixi121 – 133Combined sources13
Beta strandi136 – 138Combined sources3
Beta strandi143 – 145Combined sources3
Beta strandi147 – 154Combined sources8
Beta strandi156 – 167Combined sources12
Turni174 – 177Combined sources4
Beta strandi179 – 183Combined sources5
Beta strandi186 – 188Combined sources3
Helixi189 – 199Combined sources11
Beta strandi203 – 210Combined sources8
Turni212 – 214Combined sources3
Helixi217 – 230Combined sources14
Helixi231 – 233Combined sources3
Beta strandi237 – 243Combined sources7
Helixi245 – 254Combined sources10
Helixi257 – 259Combined sources3
Helixi260 – 278Combined sources19
Beta strandi283 – 285Combined sources3
Beta strandi290 – 292Combined sources3
Helixi298 – 304Combined sources7
Helixi305 – 307Combined sources3
Turni315 – 318Combined sources4
Helixi321 – 330Combined sources10
Helixi334 – 337Combined sources4
Helixi359 – 366Combined sources8
Helixi372 – 381Combined sources10
Beta strandi383 – 387Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C5SX-ray2.50A1-403[»]
ProteinModelPortaliQ81KU0.
SMRiQ81KU0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81KU0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 165THUMPAdd BLAST106

Sequence similaritiesi

Belongs to the ThiI family.Curated
Contains 1 THUMP domain.Curated

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
HOGENOMiHOG000227470.
KOiK03151.
OMAiEAAYISH.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81KU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYEYILVRY GEMTTKGKNR SKFVSTLKDN VKFKLKKFPN IKIDATHDRM
60 70 80 90 100
YIQLNGEDHE AVSERLKDVF GIHKFNLAMK VPSELEDIKK GALAAFLQVK
110 120 130 140 150
GDVKTFKITV HRSYKHFPMR TMELLPEIGG HILENTEDIT VDVHNPDVNV
160 170 180 190 200
RVEIRSGYSY IMCDERMGAG GLPVGVGGKV MVLLSGGIDS PVAAYLTMKR
210 220 230 240 250
GVSVEAVHFH SPPFTSERAK QKVIDLAQEL TKYCKRVTLH LVPFTEVQKT
260 270 280 290 300
INKEIPSSYS MTVMRRMMMR ITERIAEERN ALAITTGESL GQVASQTLDS
310 320 330 340 350
MHTINEVTNY PVIRPLITMD KLEIIKIAEE IGTYDISIRP YEDCCTVFTP
360 370 380 390 400
ASPATKPKRE KANRFEAKYD FTPLIDEAVA NKETMVLQTV EVVAEEEKFE

ELF
Length:403
Mass (Da):45,711
Last modified:June 1, 2003 - v1
Checksum:iA87E445661268FA6
GO

Sequence cautioni

The sequence AAT56842 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28584.1.
AE017334 Genomic DNA. Translation: AAT34017.2.
AE017225 Genomic DNA. Translation: AAT56842.1. Different initiation.
RefSeqiNP_847098.1. NC_003997.3.
YP_030792.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP28584; AAP28584; BA_4899.
AAT34017; AAT34017; GBAA_4899.
AAT56842; AAT56842; BAS4545.
GeneIDi1087273.
2850079.
KEGGiban:BA_4899.
bar:GBAA_4899.
bat:BAS4545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28584.1.
AE017334 Genomic DNA. Translation: AAT34017.2.
AE017225 Genomic DNA. Translation: AAT56842.1. Different initiation.
RefSeqiNP_847098.1. NC_003997.3.
YP_030792.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C5SX-ray2.50A1-403[»]
ProteinModelPortaliQ81KU0.
SMRiQ81KU0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_4899.

Protocols and materials databases

DNASUi1087273.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP28584; AAP28584; BA_4899.
AAT34017; AAT34017; GBAA_4899.
AAT56842; AAT56842; BAS4545.
GeneIDi1087273.
2850079.
KEGGiban:BA_4899.
bar:GBAA_4899.
bat:BAS4545.

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
HOGENOMiHOG000227470.
KOiK03151.
OMAiEAAYISH.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciANTHRA:THII-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ81KU0.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHII_BACAN
AccessioniPrimary (citable) accession number: Q81KU0
Secondary accession number(s): Q6HS96, Q6KLJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.