Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS (By similarity).By similarity

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265ATP1
Binding sitei287ATP; via amide nitrogen1
Binding sitei296ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 184ATP2
Nucleotide bindingi208 – 209ATP2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferase (EC:2.8.1.4)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene namesi
Name:thiI
Ordered Locus Names:BA_4899, GBAA_4899, BAS4545
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548301 – 403Probable tRNA sulfurtransferaseAdd BLAST403

Interactioni

Protein-protein interaction databases

STRINGi260799.BAS4545

Structurei

Secondary structure

1403
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi18 – 34Combined sources17
Turni35 – 37Combined sources3
Beta strandi42 – 45Combined sources4
Beta strandi50 – 53Combined sources4
Helixi59 – 66Combined sources8
Beta strandi72 – 84Combined sources13
Helixi85 – 97Combined sources13
Beta strandi105 – 112Combined sources8
Helixi121 – 133Combined sources13
Beta strandi136 – 138Combined sources3
Beta strandi143 – 145Combined sources3
Beta strandi147 – 154Combined sources8
Beta strandi156 – 167Combined sources12
Turni174 – 177Combined sources4
Beta strandi179 – 183Combined sources5
Beta strandi186 – 188Combined sources3
Helixi189 – 199Combined sources11
Beta strandi203 – 210Combined sources8
Turni212 – 214Combined sources3
Helixi217 – 230Combined sources14
Helixi231 – 233Combined sources3
Beta strandi237 – 243Combined sources7
Helixi245 – 254Combined sources10
Helixi257 – 259Combined sources3
Helixi260 – 278Combined sources19
Beta strandi283 – 285Combined sources3
Beta strandi290 – 292Combined sources3
Helixi298 – 304Combined sources7
Helixi305 – 307Combined sources3
Turni315 – 318Combined sources4
Helixi321 – 330Combined sources10
Helixi334 – 337Combined sources4
Helixi359 – 366Combined sources8
Helixi372 – 381Combined sources10
Beta strandi383 – 387Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C5SX-ray2.50A1-403[»]
ProteinModelPortaliQ81KU0
SMRiQ81KU0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81KU0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 165THUMPAdd BLAST106

Sequence similaritiesi

Belongs to the ThiI family.Curated

Phylogenomic databases

eggNOGiENOG4105D8I Bacteria
COG0301 LUCA
HOGENOMiHOG000227470
KOiK03151
OMAiKLFPEIM

Family and domain databases

CDDicd01712 ThiI, 1 hit
Gene3Di3.40.50.6201 hit
HAMAPiMF_00021 ThiI, 1 hit
InterProiView protein in InterPro
IPR014729 Rossmann-like_a/b/a_fold
IPR020536 ThiI_AANH
IPR004114 THUMP_dom
IPR003720 tRNA_STrfase
PfamiView protein in Pfam
PF02568 ThiI, 1 hit
PF02926 THUMP, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
TIGRFAMsiTIGR00342 TIGR00342, 1 hit
PROSITEiView protein in PROSITE
PS51165 THUMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q81KU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYEYILVRY GEMTTKGKNR SKFVSTLKDN VKFKLKKFPN IKIDATHDRM
60 70 80 90 100
YIQLNGEDHE AVSERLKDVF GIHKFNLAMK VPSELEDIKK GALAAFLQVK
110 120 130 140 150
GDVKTFKITV HRSYKHFPMR TMELLPEIGG HILENTEDIT VDVHNPDVNV
160 170 180 190 200
RVEIRSGYSY IMCDERMGAG GLPVGVGGKV MVLLSGGIDS PVAAYLTMKR
210 220 230 240 250
GVSVEAVHFH SPPFTSERAK QKVIDLAQEL TKYCKRVTLH LVPFTEVQKT
260 270 280 290 300
INKEIPSSYS MTVMRRMMMR ITERIAEERN ALAITTGESL GQVASQTLDS
310 320 330 340 350
MHTINEVTNY PVIRPLITMD KLEIIKIAEE IGTYDISIRP YEDCCTVFTP
360 370 380 390 400
ASPATKPKRE KANRFEAKYD FTPLIDEAVA NKETMVLQTV EVVAEEEKFE

ELF
Length:403
Mass (Da):45,711
Last modified:June 1, 2003 - v1
Checksum:iA87E445661268FA6
GO

Sequence cautioni

The sequence AAT56842 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP28584.1
AE017334 Genomic DNA Translation: AAT34017.2
AE017225 Genomic DNA Translation: AAT56842.1 Different initiation.
RefSeqiNP_847098.1, NC_003997.3
YP_030792.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP28584; AAP28584; BA_4899
AAT34017; AAT34017; GBAA_4899
AAT56842; AAT56842; BAS4545
GeneIDi1087273
2850079
KEGGiban:BA_4899
bar:GBAA_4899
bat:BAS4545
PATRICifig|198094.11.peg.4859

Similar proteinsi

Entry informationi

Entry nameiTHII_BACAN
AccessioniPrimary (citable) accession number: Q81KU0
Secondary accession number(s): Q6HS96, Q6KLJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome