Q81KR6 (MSCL_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	BA_4924, GBAA_4924, BAS4569

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_0000237969
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	67 – 87	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81KR6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6B7EDF384FBED5EC	FASTA	132	14,811
	10 20 30 40 50 60 MWNEFKKFAF KGNVIDLAVG VVIGAAFGKI VSSLVKDIIT PLLGMVLGGV DFTDLKITFG 70 80 90 100 110 120 KSSIMYGNFI QTIFDFLIIA AAIFMFVKVF NKLTSKREEE KEEEIPEPTK EEELLGEIRD 130 LLKQQNSSKD RA

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28608.1. AE017334 Genomic DNA. Translation: AAT34042.1. AE017225 Genomic DNA. Translation: AAT56866.1.
RefSeq	NP_847122.1. NC_003997.3. YP_021567.1. NC_007530.2. YP_030816.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81KR6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000013276; EBBACP00000013032; EBBACG00000013268. EBBACT00000013670; EBBACP00000013291; EBBACG00000013662. EBBACT00000019722; EBBACP00000019207; EBBACG00000019713.
GeneID	1084114. 2819786. 2847927.
GenomeReviews	Gene locus BA_4924 in contig AE016879_GR. Gene locus BAS4569 in contig AE017225_GR. Gene locus GBAA_4924 in contig AE017334_GR.
KEGG	ban:BA_4924. bar:GBAA_4924. bat:BAS4569.
TIGR	BA_4924. GBAA_4924.
Phylogenomic databases
GeneTree	EBGT00050000002096.
HOGENOM	HBG727604.
OMA	VIDFVIV.
ProtClustDB	PRK13955.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4569-MONOMER. BANT261594:GBAA4924-MONOMER.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_BACAN

Accession

Primary (citable) accession number: Q81KR6
Secondary accession number(s): Q6HS72, Q6KLH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program