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Q81KF3 (LUXS_BACAN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-ribosylhomocysteine lyase

EC=4.4.1.21
Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS
Gene names
Name:luxS
Ordered Locus Names:BA_5047, GBAA_5047, BAS4687
OrganismBacillus anthracis
Taxonomic identifier1392 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091

Catalytic activity

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091

Cofactor

Binds 1 iron ion per subunit By similarity. HAMAP MF_00091

Subunit structure

Homodimer By similarity. HAMAP MF_00091

Sequence similarities

Belongs to the LuxS family.

Ontologies

Keywords
   Biological processAutoinducer synthesis
Quorum sensing
   LigandIron
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processquorum sensing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionS-ribosylhomocysteine lyase activity

Inferred from electronic annotation. Source: EC

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157S-ribosylhomocysteine lyase HAMAP MF_00091
PRO_0000172204

Sites

Metal binding541Iron By similarity
Metal binding581Iron By similarity
Metal binding1261Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q81KF3 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5D2BB8F36D754793

FASTA15717,856
        10         20         30         40         50         60 
MPSVESFELD HTIVKAPYVR HCGVHNVGSD GIVNKFDIRF CQPNKQAMKP DVIHTLEHLL 

        70         80         90        100        110        120 
AFNLRKYIDR YPHFDIIDIS PMGCQTGYYL VVSGTPTVRE IIDLLELTLK DAVQITEIPA 

       130        140        150 
ANETQCGQAK LHDLEGAKRL MNFWLSQDKD ELEKVFG 

« Hide

References

[1]"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. expand/collapse author list , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
Nature 423:81-86(2003) [PubMed: 12721629] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames / isolate Porton.
[2]"Bacillus anthracis comparative genomics."
Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames ancestor.
[3]"Complete genome sequence of Bacillus anthracis Sterne."
Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sterne.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016879 Genomic DNA. Translation: AAP28724.1.
AE017334 Genomic DNA. Translation: AAT34170.1.
AE017225 Genomic DNA. Translation: AAT56981.1.
RefSeqNP_847238.1. NC_003997.3.
YP_021695.1. NC_007530.2.
YP_030931.1. NC_005945.1.

3D structure databases

ProteinModelPortalQ81KF3.
SMRQ81KF3. Positions 4-157.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000009808; EBBACP00000009564; EBBACG00000009800.
EBBACT00000014671; EBBACP00000014292; EBBACG00000014663.
EBBACT00000024054; EBBACP00000023539; EBBACG00000024045.
GeneID1084317.
2818604.
2852831.
GenomeReviewsGene locus BA_5047 in contig AE016879_GR.
Gene locus BAS4687 in contig AE017225_GR.
Gene locus GBAA_5047 in contig AE017334_GR.
KEGGban:BA_5047.
bar:GBAA_5047.
bat:BAS4687.
TIGRBA_5047.
GBAA_5047.

Phylogenomic databases

GeneTreeEBGT00050000002559.
HOGENOMHBG347473.
OMAPHGDTIT.
ProtClustDBPRK02260.

Enzyme and pathway databases

BioCycBANT260799:BAS4687-MONOMER.
BANT261594:GBAA5047-MONOMER.

Family and domain databases

HAMAPMF_00091. LuxS.
[Tree]
InterProIPR011249. Metalloenz_metal-bd.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
Gene3DG3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KOK07173.
PfamPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFPIRSF006160. AI2. 1 hit.
PRINTSPR01487. LUXSPROTEIN.
ProDomPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLUXS_BACAN
AccessionPrimary (citable) accession number: Q81KF3
Secondary accession number(s): Q6HRV7, Q6KL63
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families