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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei309 – 3091NucleophileUniRule annotation
Active sitei352 – 3521Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciANTHRA:GLGB-MONOMER.
BANT260799:GJAJ-4815-MONOMER.
BANT261594:GJ7F-4975-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:BA_5123, GBAA_5123, BAS4761
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000005639 Componenti: Chromosome UP000000594 Componenti: Chromosome UP000000427 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6456451,4-alpha-glucan branching enzyme GlgBPRO_0000188675Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ81K82. 1 interaction.
STRINGi198094.BA_5123.

Structurei

3D structure databases

ProteinModelPortaliQ81K82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81K82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVINCEEVK RDEFHTEKYY ESYNIFGAHI VTEDEMRGVR FTVWAPHAKA
60 70 80 90 100
MSVVGDFNEW DYEQHKMLQV TEEGIWSLFI PHIEEREIYK YAIETMAGDV
110 120 130 140 150
IFKADPYAVY AEVRPNTASV VFDIKGYEWN DKNWSRKKKK KSVYKEAMTV
160 170 180 190 200
YELHFGSWKK KEDGTLYSYR EMAEELIPYV VEHQFTHIEI MPLVEHPYDR
210 220 230 240 250
SWGYQGTGYY AATSRFGTPH DLMHFVDECH KYGIGVILDW VPGHFCKDAH
260 270 280 290 300
GLYLFDGTPT YEYKDKDVQE NPVWGTVNFD LGKREVRNFL ISNALFWMRY
310 320 330 340 350
FHIDGFRVDA VANMLYWNKE GQEQSNEHAV SFLRELNEAV FAEDEDFLMT
360 370 380 390 400
AEDSTAWPLV TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHDK
410 420 430 440 450
MTFSLLYAYS ENFILPLSHD EVVHGKKSLL NKMPGDYWDK FAQLRLLYGY
460 470 480 490 500
FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW NLHDFEMHRY MHDYFKELIA
510 520 530 540 550
LYKRSKPLWQ LDHSREGFQW IDANNNEQSI FSFIRQGDKQ EDALVVVCNF
560 570 580 590 600
TKATYENYKV GVPDFEYYNE VLNSDAEQYG GSGQVNKKRL KTFQEPYHNQ
610 620 630 640
TAHVEITIPP FGVSILRPVK TRKGSKKQDG SKTKVRSNVT SRGKR
Length:645
Mass (Da):76,099
Last modified:May 31, 2003 - v1
Checksum:i115F542FD84A4A6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28795.1.
AE017334 Genomic DNA. Translation: AAT34251.2.
AE017225 Genomic DNA. Translation: AAT57055.1.
RefSeqiNP_847309.1. NC_003997.3.
WP_000111381.1. NZ_KN050651.1.
YP_021776.2. NC_007530.2.
YP_031005.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP28795; AAP28795; BA_5123.
AAT34251; AAT34251; GBAA_5123.
AAT57055; AAT57055; BAS4761.
GeneIDi1084438.
2850585.
KEGGiban:BA_5123.
bar:GBAA_5123.
bat:BAS4761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28795.1.
AE017334 Genomic DNA. Translation: AAT34251.2.
AE017225 Genomic DNA. Translation: AAT57055.1.
RefSeqiNP_847309.1. NC_003997.3.
WP_000111381.1. NZ_KN050651.1.
YP_021776.2. NC_007530.2.
YP_031005.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ81K82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ81K82. 1 interaction.
STRINGi198094.BA_5123.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASUi1084438.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP28795; AAP28795; BA_5123.
AAT34251; AAT34251; GBAA_5123.
AAT57055; AAT57055; BAS4761.
GeneIDi1084438.
2850585.
KEGGiban:BA_5123.
bar:GBAA_5123.
bat:BAS4761.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciANTHRA:GLGB-MONOMER.
BANT260799:GJAJ-4815-MONOMER.
BANT261594:GJ7F-4975-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiGLGB_BACAN
AccessioniPrimary (citable) accession number: Q81K82
Secondary accession number(s): Q6HRN3, Q6KKZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2003
Last sequence update: May 31, 2003
Last modified: March 31, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.