Q81K82 (GLGB_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	BA_5123, GBAA_5123, BAS4761

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000188675
Sites
	Active site	309	1	Nucleophile By similarity
	Active site	352	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81K82 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 115F542FD84A4A6D	FASTA	645	76,099
	10 20 30 40 50 60 MSVINCEEVK RDEFHTEKYY ESYNIFGAHI VTEDEMRGVR FTVWAPHAKA MSVVGDFNEW 70 80 90 100 110 120 DYEQHKMLQV TEEGIWSLFI PHIEEREIYK YAIETMAGDV IFKADPYAVY AEVRPNTASV 130 140 150 160 170 180 VFDIKGYEWN DKNWSRKKKK KSVYKEAMTV YELHFGSWKK KEDGTLYSYR EMAEELIPYV 190 200 210 220 230 240 VEHQFTHIEI MPLVEHPYDR SWGYQGTGYY AATSRFGTPH DLMHFVDECH KYGIGVILDW 250 260 270 280 290 300 VPGHFCKDAH GLYLFDGTPT YEYKDKDVQE NPVWGTVNFD LGKREVRNFL ISNALFWMRY 310 320 330 340 350 360 FHIDGFRVDA VANMLYWNKE GQEQSNEHAV SFLRELNEAV FAEDEDFLMT AEDSTAWPLV 370 380 390 400 410 420 TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHDK MTFSLLYAYS ENFILPLSHD 430 440 450 460 470 480 EVVHGKKSLL NKMPGDYWDK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 490 500 510 520 530 540 NLHDFEMHRY MHDYFKELIA LYKRSKPLWQ LDHSREGFQW IDANNNEQSI FSFIRQGDKQ 550 560 570 580 590 600 EDALVVVCNF TKATYENYKV GVPDFEYYNE VLNSDAEQYG GSGQVNKKRL KTFQEPYHNQ 610 620 630 640 TAHVEITIPP FGVSILRPVK TRKGSKKQDG SKTKVRSNVT SRGKR

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28795.1. AE017334 Genomic DNA. Translation: AAT34251.2. AE017225 Genomic DNA. Translation: AAT57055.1.
RefSeq	NP_847309.1. NC_003997.3. YP_021776.2. NC_007530.2. YP_031005.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81K82.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q81K82. 1 interaction.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000011004; EBBACP00000010760; EBBACG00000010996. EBBACT00000018572; EBBACP00000018193; EBBACG00000018563. EBBACT00000022867; EBBACP00000022352; EBBACG00000022858.
GeneID	1084438. 2819765. 2850585.
GenomeReviews	Gene locus BA_5123 in contig AE016879_GR. Gene locus BAS4761 in contig AE017225_GR. Gene locus GBAA_5123 in contig AE017334_GR.
KEGG	ban:BA_5123. bar:GBAA_5123. bat:BAS4761.
TIGR	BA_5123. GBAA_5123.
Phylogenomic databases
GeneTree	EBGT00070000031950.
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4761-MONOMER. BANT261594:GBAA5123-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACAN

Accession

Primary (citable) accession number: Q81K82
Secondary accession number(s): Q6HRN3, Q6KKZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program