Reviewed, UniProtKB/Swiss-Prot Q81K75 (G6PI_BACAN)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate isomerase
      Short name=GPI
    EC=5.3.1.9
Alternative name(s):
    Phosphoglucose isomerase
      Short name=PGI
    Phosphohexose isomerase
      Short name=PHI

Gene names

Name:	pgi
Ordered Locus Names:	BA_5130, GBAA_5130, BAS4767

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP MF_00473
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP MF_00473
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GPI family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Glucose-6-phosphate isomerase HAMAP MF_00473		PRO_0000180584
Sites
	Active site	291	1	Proton donor By similarity
	Active site	312	1	By similarity
	Active site	426	1	By similarity
Amino acid modifications
	Modified residue	39	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81K75-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A8F91E74160A80D9	FASTA	450	50,345
	10 20 30 40 50 60 MSTHVTFDYS KALSFIGEHE ITYLRDAVKV THHAIHEKTG AGNDFLGWVD LPLQYDKEEF 70 80 90 100 110 120 ARIQKCAEKI KNDSDILLVV GIGGSYLGAR AAIEMLNHSF YNTLSKEQRK TPQVLFVGQN 130 140 150 160 170 180 ISSTYMKDLM DVLEGKDFSI NVISKSGTTT EPALAFRIFR KLLEEKYGKE EARKRIYATT 190 200 210 220 230 240 DKARGALKTL ADNEGYETFV IPDDVGGRFS VLTPVGLLPI AVSGLNIEEM MKGAAAGRDD 250 260 270 280 290 300 FGTSELEENP AYQYAVVRNA LYNKGKTIEM LINYEPALQY FAEWWKQLFG ESEGKDQKGI 310 320 330 340 350 360 FPSSANFSTD LHSLGQYVQE GRRDLFETVL KVGKSTHELT IESEENDLDG LNYLAGETVD 370 380 390 400 410 420 FVNTKAYEGT LLAHSDGGVP NLIVNIPELN EYTFGYLVYF FEKACAMSGY LLGVNPFDQP 430 440 450 GVEAYKKNMF ALLGKPGFEE LKAELEERLK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE016879 Genomic DNA. Translation: AAP28802.1.
AE017334 Genomic DNA. Translation: AAT34259.1.
AE017225 Genomic DNA. Translation: AAT57060.1.

RefSeq

NP_847316.1.
YP_021784.1.
YP_031010.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IFS	X-ray	2.00	A/B/C/D/E/F	1-450	[»]

ModBase

Search...

Genome annotation databases

GeneID

1084495.
2815760.
2849040.

GenomeReviews

Gene locus BA_5130 in contig AE016879_GR.
Gene locus BAS4767 in contig AE017225_GR.
Gene locus GBAA_5130 in contig AE017334_GR.

KEGG

ban:BA5130.
bar:GBAA5130.
bat:BAS4767.

TIGR

BA_5130.
GBAA_5130.

Phylogenomic databases

HOGENOM

Q81K75.

OMA

NPRKNVI.

Enzyme and pathway databases

BioCyc

BANT260799:BAS4767-MON.
BANT261594:GBAA5130-MON.

BRENDA

5.3.1.9. 267517.

Family and domain databases

HAMAP

MF_00473.
[Tree]

InterPro

IPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]

PANTHER

PTHR11469. G6P_Isomerase. 1 hit.

Pfam

PF00342. PGI. 1 hit.
[Graphical view]

PRINTS

PR00662. G6PISOMERASE.

PROSITE

PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

G6PI_BACAN

Accession

Primary (citable) accession number: Q81K75
Secondary accession number(s): Q6HRM8, Q6KKZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)