Q81JY0 (PTGA_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-specific phosphotransferase enzyme IIA component
EC=2.7.1.-

Alternative name(s):
EIIA-Glc
EIII-Glc
PTS system glucose-specific EIIA component

Gene names

Name:	crr
Ordered Locus Names:	BA_5563, GBAA_5563, BAS5169

Organism

Bacillus anthracis

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cytoplasm By similarity.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Glucose-specific phosphotransferase enzyme IIA component		PRO_0000186543
Regions
	Domain	33 – 137	105	PTS EIIA type-1
Sites
	Active site	85	1	Tele-phosphohistidine intermediate By similarity
	Site	70	1	Important for phospho-donor activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81JY0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 41935CF553BA525D	FASTA	165	17,475
	10 20 30 40 50 60 MFKKLFGLGS KTNEETIVAP LTGAVKNIEE VPDPVFAGRM MGDGVAIDPT EGVVVSPVDG 70 80 90 100 110 120 EIVQLFHTKH AIGIKAKNGT EILIHVGLET VKMEGEGFEA HVSEGQAVKA GDKLISFDLE 130 140 150 160 LIREKAKSTI TPIVITNTDA AESIKTTVGV TATKGSTEVM KVTMK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP29206.1. AE017334 Genomic DNA. Translation: AAT34706.1. AE017225 Genomic DNA. Translation: AAT57458.1.
RefSeq	NP_847720.1. NC_003997.3. YP_022231.1. NC_007530.2. YP_031408.1. NC_005945.1.
3D structure databases
ProteinModelPortal	Q81JY0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000013352; EBBACP00000013108; EBBACG00000013344. EBBACT00000015942; EBBACP00000015563; EBBACG00000015934. EBBACT00000020713; EBBACP00000020198; EBBACG00000020704.
GeneID	1085250. 2820053. 2851729.
GenomeReviews	Gene locus BA_5563 in contig AE016879_GR. Gene locus BAS5169 in contig AE017225_GR. Gene locus GBAA_5563 in contig AE017334_GR.
KEGG	ban:BA_5563. bar:GBAA_5563. bat:BAS5169.
TIGR	BA_5563. GBAA_5563.
Phylogenomic databases
GeneTree	EBGT00050000000034.
HOGENOM	HBG517865.
OMA	RRLISGQ.
ProtClustDB	CLSK917671.
Enzyme and pathway databases
BioCyc	BANT260799:BAS5169-MONOMER. BANT261594:GBAA5563-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR001127. PTS_EIIA_1_perm. [Graphical view]
KO	K02777.
Pfam	PF00358. PTS_EIIA_1. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit.
TIGRFAMs	TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTGA_BACAN

Accession

Primary (citable) accession number: Q81JY0
Secondary accession number(s): Q6HQI0, Q6KJV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program