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Q81JX0

- KITH_BACAN

UniProt

Q81JX0 - KITH_BACAN

Protein

Thymidine kinase

Gene

tdk

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Proton acceptorUniRule annotation
    Binding sitei120 – 1201Substrate; via amide nitrogen
    Metal bindingi145 – 1451Zinc
    Metal bindingi148 – 1481Zinc
    Binding sitei179 – 1791SubstrateBy similarity
    Metal bindingi183 – 1831Zinc
    Metal bindingi186 – 1861Zinc

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228ATPUniRule annotation
    Nucleotide bindingi88 – 914ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. thymidine kinase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciANTHRA:TDK-MONOMER.
    BANT260799:GJAJ-5254-MONOMER.
    BANT261594:GJ7F-5433-MONOMER.
    MetaCyc:MONOMER-17898.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
    Gene namesi
    Name:tdkUniRule annotation
    Ordered Locus Names:BA_5573, GBAA_5573, BAS5179
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 194194Thymidine kinasePRO_0000174953Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    IntActiQ81JX0. 5 interactions.
    STRINGi198094.BA_5573.

    Structurei

    Secondary structure

    1
    194
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 146
    Helixi21 – 3414
    Beta strandi39 – 435
    Beta strandi65 – 673
    Helixi71 – 777
    Beta strandi84 – 874
    Helixi90 – 923
    Helixi97 – 10610
    Beta strandi110 – 1156
    Helixi128 – 1347
    Beta strandi136 – 1405
    Turni146 – 1483
    Beta strandi149 – 1513
    Beta strandi154 – 1596
    Turni176 – 1783
    Beta strandi179 – 1824
    Turni184 – 1863

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2J9RX-ray2.70A1-194[»]
    ProteinModelPortaliQ81JX0.
    SMRiQ81JX0. Positions 1-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ81JX0.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni170 – 1745Substrate binding

    Sequence similaritiesi

    Belongs to the thymidine kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1435.
    HOGENOMiHOG000076390.
    KOiK00857.
    OMAiQAICLSC.
    OrthoDBiEOG69D3J2.

    Family and domain databases

    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81JX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYLINQNGWI EVICGSMFSG KSEELIRRVR RTQFAKQHAI VFKPCIDNRY    50
    SEEDVVSHNG LKVKAVPVSA SKDIFKHITE EMDVIAIDEV QFFDGDIVEV 100
    VQVLANRGYR VIVAGLDQDF RGLPFGQVPQ LMAIAEHVTK LQAVCSACGS 150
    PASRTQRLID GEPAAFDDPI ILVGASESYE PRCRHCHAVP TKQR 194
    Length:194
    Mass (Da):21,642
    Last modified:June 1, 2003 - v1
    Checksum:iD2A9B182355D9D13
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP29216.1.
    AE017334 Genomic DNA. Translation: AAT34716.1.
    AE017225 Genomic DNA. Translation: AAT57468.1.
    RefSeqiNP_847730.1. NC_003997.3.
    YP_022241.1. NC_007530.2.
    YP_031418.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP29216; AAP29216; BA_5573.
    AAT34716; AAT34716; GBAA_5573.
    AAT57468; AAT57468; BAS5179.
    GeneIDi1085264.
    2819897.
    2851937.
    KEGGiban:BA_5573.
    bar:GBAA_5573.
    bat:BAS5179.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP29216.1 .
    AE017334 Genomic DNA. Translation: AAT34716.1 .
    AE017225 Genomic DNA. Translation: AAT57468.1 .
    RefSeqi NP_847730.1. NC_003997.3.
    YP_022241.1. NC_007530.2.
    YP_031418.1. NC_005945.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2J9R X-ray 2.70 A 1-194 [» ]
    ProteinModelPortali Q81JX0.
    SMRi Q81JX0. Positions 1-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q81JX0. 5 interactions.
    STRINGi 198094.BA_5573.

    Protocols and materials databases

    DNASUi 1085264.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP29216 ; AAP29216 ; BA_5573 .
    AAT34716 ; AAT34716 ; GBAA_5573 .
    AAT57468 ; AAT57468 ; BAS5179 .
    GeneIDi 1085264.
    2819897.
    2851937.
    KEGGi ban:BA_5573.
    bar:GBAA_5573.
    bat:BAS5179.

    Phylogenomic databases

    eggNOGi COG1435.
    HOGENOMi HOG000076390.
    KOi K00857.
    OMAi QAICLSC.
    OrthoDBi EOG69D3J2.

    Enzyme and pathway databases

    BioCyci ANTHRA:TDK-MONOMER.
    BANT260799:GJAJ-5254-MONOMER.
    BANT261594:GJ7F-5433-MONOMER.
    MetaCyc:MONOMER-17898.

    Miscellaneous databases

    EvolutionaryTracei Q81JX0.

    Family and domain databases

    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.
    3. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.
    4. "Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding."
      Kosinska U., Carnrot C., Sandrini M.P., Clausen A.R., Wang L., Piskur J., Eriksson S., Eklund H.
      FEBS J. 274:727-737(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND DEOXYTHYMIDINE, SUBUNIT.

    Entry informationi

    Entry nameiKITH_BACAN
    AccessioniPrimary (citable) accession number: Q81JX0
    Secondary accession number(s): Q6HQH0, Q6KJU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3