Reviewed, UniProtKB/Swiss-Prot Q81JT1 (SPEB_BACAN)

Names and origin

Protein names

Recommended name:
    Agmatinase
    EC=3.5.3.11
Alternative name(s):
    Agmatine ureohydrolase
      Short name=AUH

Gene names

Name:	speB
Ordered Locus Names:	BA_5617, GBAA_5617, BAS5218

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine By similarity.
Catalytic activity	Agmatine + H2O = putrescine + urea.
Cofactor	Manganese By similarity.
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1.
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	agmatinase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Agmatinase		PRO_0000173725
Sites
	Metal binding	112	1	Manganese By similarity
	Metal binding	135	1	Manganese By similarity
	Metal binding	137	1	Manganese By similarity
	Metal binding	139	1	Manganese By similarity
	Metal binding	216	1	Manganese By similarity
	Metal binding	218	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81JT1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6F2F39ED33E38DE4	FASTA	290	32,390
	10 20 30 40 50 60 MRFDEAYSGK VFIKSHPSFE ESEVVIYGMP MDWTVSYRPG SRFGPARIRE VSIGLEEYSP 70 80 90 100 110 120 YLDRELEEVK YFDAGDIPLP FGNAQRSLDM IEEYVSKLLD AGKFPLGLGG EHLVSWPIFK 130 140 150 160 170 180 AMAKKYPDLA IIHMDAHTDL RESYEGEPLS HSTPIRKVCD LIGPENVYSF GIRSGMKEEF 190 200 210 220 230 240 EWAKEVGMNL YKFDVLEPLK EVLPKLEGRP VYVTIDIDVL DPAHAPGTGT LEAGGITSKE 250 260 270 280 290 LLDSIVAIAN SNINVVGADL VEVAPVYDHS DQTPVAASKF VREMLLGWVK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP29255.1. AE017334 Genomic DNA. Translation: AAT34764.1. AE017225 Genomic DNA. Translation: AAT57507.1.
RefSeq	NP_847769.1. YP_022289.1. YP_031457.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQ6 based on UniProtKB P37819.
ModBase	Search...
Genome annotation databases
GeneID	1085325. 2817842. 2848265.
GenomeReviews	Gene locus BA_5617 in contig AE016879_GR. Gene locus BAS5218 in contig AE017225_GR. Gene locus GBAA_5617 in contig AE017334_GR.
KEGG	ban:BA5617. bar:GBAA5617. bat:BAS5218.
TIGR	BA_5617. GBAA_5617.
Phylogenomic databases
HOGENOM	Q81JT1.
OMA	RARWRED.
Enzyme and pathway databases
BioCyc	BANT260799:BAS5218-MON. BANT261594:GBAA5617-MON.
BRENDA	3.5.3.11. 267517.
Family and domain databases
InterPro	IPR005925. Agmatinase. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01230. agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_BACAN

Accession

Primary (citable) accession number: Q81JT1
Secondary accession number(s): Q6HQD1, Q6KJQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)