Reviewed, UniProtKB/Swiss-Prot Q81JJ9 (GUAC_BACAN)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	BA_5705, GBAA5705, BAS5309

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 327

327

GMP reductase

PRO_0000093746

Regions

Nucleotide binding

204 – 227

24

NADP By similarity

Sites

Active site

175

1

Thioimidate intermediate By similarity

Secondary structure

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327

Helix Strand Turn

Details...

Helix

7 – 9

3

Beta strand

10 – 12

3

Helix

22 – 24

3

Beta strand

29 – 31

3

Beta strand

34 – 42

9

Turn

46 – 48

3

Helix

51 – 59

9

Helix

71 – 73

3

Helix

74 – 83

10

Beta strand

89 – 92

4

Helix

96 – 107

12

Beta strand

113 – 118

6

Helix

125 – 137

13

Beta strand

141 – 148

8

Helix

151 – 160

10

Beta strand

163 – 167

5

Helix

176 – 181

6

Beta strand

182 – 185

4

Helix

189 – 198

10

Beta strand

204 – 208

5

Helix

214 – 221

8

Beta strand

225 – 230

6

Helix

231 – 233

3

Beta strand

239 – 241

3

Beta strand

273 – 277

5

Helix

282 – 299

18

Beta strand

302 – 304

3

Helix

305 – 310

6

Beta strand

313 – 315

3

Sequences

	Sequence		Length	Mass (Da)
	Q81JJ9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8006FDFA6A5F2E78	FASTA	327	36,076
	10 20 30 40 50 60 MGNVFDYEDI QLIPAKCIVN SRSECDTTVT LGKHKFKLPV VPANMQTIID ERIATYLAEN 70 80 90 100 110 120 NYFYIMHRFQ PEKRISFIRD MQSRGLIASI SVGVKEDEYE FVQQLAAEHL TPEYITIDIA 130 140 150 160 170 180 HGHSNAVINM IQHIKKHLPE SFVIAGNVGT PEAVRELENA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCAKAA SKPIIADGGI RTNGDVAKSI RFGATMVMIG SLFAGHEESP 250 260 270 280 290 300 GETIEKDGKL YKEYFGSASE FQKGEKKNVE GKKMFVEHKG SLEDTLIEME QDLQSSISYA 310 320 GGTKLDSIRT VDYVVVKNSI FNGDKVY

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE016879 Genomic DNA. Translation: AAP29337.1.
AE017334 Genomic DNA. Translation: AAT34865.1.
AE017225 Genomic DNA. Translation: AAT57596.1.

RefSeq

NP_847851.1.
YP_022390.1.
YP_031546.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YPF	X-ray	1.80	A/B	1-327	[»]
2A1Y	X-ray	2.27	A	1-327	[»]

ModBase

Search...

Genome annotation databases

GeneID

1085456.
2816139.
2852911.

GenomeReviews

Gene locus BA_5705 in contig AE016879_GR.
Gene locus BAS5309 in contig AE017225_GR.
Gene locus GBAA5705 in contig AE017334_GR.

KEGG

ban:BA5705.
bar:GBAA5705.
bat:BAS5309.

TIGR

BA_5705.
GBAA5705.

Phylogenomic databases

HOGENOM

Q81JJ9.

Enzyme and pathway databases

BioCyc

BANT260799:BAS5309-MON.
BANT261594:GBAA5705-MON.

Family and domain databases

HAMAP

MF_01511.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR005994. GMP_reduct2.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DHase_GMPRtase.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

Pfam

PF00478. IMPDH. 1 hit.
[Graphical view]

PIRSF

PIRSF036500. GMP_red_Firmic. 1 hit.

TIGRFAMs

TIGR01306. GMP_reduct_2. 1 hit.

PROSITE

PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GUAC_BACAN

Accession

Primary (citable) accession number: Q81JJ9
Secondary accession number(s): Q6HQ42, Q6KJI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)