Reviewed, UniProtKB/Swiss-Prot Q81JG3 (ACPS_BACAN)

Names and origin

Protein names

Recommended name:
    Holo-[acyl-carrier-protein] synthase
      Short name=Holo-ACP synthase
    EC=2.7.8.7
Alternative name(s):
    4'-phosphopantetheinyl transferase acpS

Gene names

Name:	acpS
Ordered Locus Names:	BA_0250, GBAA_0250, BAS0236

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein By similarity.
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Holo-[acyl-carrier-protein] synthase HAMAP MF_00101		PRO_0000175606
Sites
	Metal binding	8	1	Magnesium By similarity
	Metal binding	58	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81JG3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8680A0B258813E14	FASTA	119	13,100
	10 20 30 40 50 60 MIVGIGIDII ELNRIEKMLD GKLKFMERIL TENERNVAKG LKGSRLTEFV AGRFAAKEAY 70 80 90 100 110 SKAVGTGIGK EVSFLDIEVR NDDRGKPILI TSTEHIVHLS ISHSKEFAVA QVVLESSSS

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE016879 Genomic DNA. Translation: AAP24289.1.
AE017334 Genomic DNA. Translation: AAT29331.1.
AE017225 Genomic DNA. Translation: AAT52572.1.

RefSeq

NP_842803.1.
YP_016856.1.
YP_026521.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HYK	X-ray	2.31	A/B/C	1-119	[»]

ModBase

Search...

Genome annotation databases

GeneID

1087043.
2819988.
2850731.

GenomeReviews

Gene locus BA_0250 in contig AE016879_GR.
Gene locus BAS0236 in contig AE017225_GR.
Gene locus GBAA_0250 in contig AE017334_GR.

KEGG

ban:BA0250.
bar:GBAA0250.
bat:BAS0236.

TIGR

BA_0250.
GBAA_0250.

Phylogenomic databases

HOGENOM

Q81JG3.

OMA

VHVSITH.

Enzyme and pathway databases

BioCyc

BANT260799:BAS0236-MON.
BANT261594:GBAA0250-MON.

BRENDA

2.7.8.7. 267517.

Family and domain databases

HAMAP

MF_00101.
[Tree]

InterPro

IPR008278. 4-PPantetheinyl_Trfase.
IPR002582. ACPS.
IPR004568. PPantethiene-prot_Trfase.
[Graphical view]

Pfam

PF01648. ACPS. 1 hit.
[Graphical view]

ProDom

PD004282. ACPS. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00516. acpS. 1 hit.
TIGR00556. pantethn_trn. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ACPS_BACAN

Accession

Primary (citable) accession number: Q81JG3
Secondary accession number(s): Q6I4F9, Q6KY61

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)