Reviewed, UniProtKB/Swiss-Prot Q81JF6 (FABH2_BACAN)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2
    EC=2.3.1.180
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III protein 2
    Beta-ketoacyl-ACP synthase III 2
      Short name=KAS III 2

Gene names

Name:	fabH2
Ordered Locus Names:	BA_1826, GBAA_1826, BAS1691

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2 HAMAP MF_01815		PRO_0000110394
Regions
	Region	252 – 256	5	ACP-binding By similarity
Sites
	Active site	114	1	By similarity
	Active site	251	1	By similarity
	Active site	281	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81JF6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 342E6463194A011E	FASTA	327	36,280
	10 20 30 40 50 60 MNSKSRITAI GTHVPNQILS NNDLEKMIDT NDEWIVQRTG MKERRIASED EYSSNLAIKA 70 80 90 100 110 120 VENLCTTYKK NLEDVDCIIV ATTTADYVFP SVACQIQQYF NIPHTMAFDL NATCAGFTYG 130 140 150 160 170 180 LHVGNSLITS GSHKKVLVVA TETLSKVTDY TDRTTCILFG DGAGAILLER DENKPSFIAY 190 200 210 220 230 240 HMGTNGHGGI HLYRTNLSTT MNDTPLQTNE KIVQNGREVY KWATRTVPVG IKELLHTANM 250 260 270 280 290 300 KMDDIDWFIP HSANLRMIES ICEKSQIPIH KTLTSVEYMG NTSSVSIPLA LDLARKKGKL 310 320 NNGDTLLLYG FGGGLTHLGL IVEWDLS

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP25732.1. AE017334 Genomic DNA. Translation: AAT30939.1. AE017225 Genomic DNA. Translation: AAT54008.1.
RefSeq	NP_844246.1. YP_018464.1. YP_027957.1.
3D structure databases
SMR	Q81JF6. Positions 5-325.
ModBase	Search...
Genome annotation databases
GeneID	1086168. 2817393. 2851898.
GenomeReviews	Gene locus BA_1826 in contig AE016879_GR. Gene locus BAS1691 in contig AE017225_GR. Gene locus GBAA_1826 in contig AE017334_GR.
KEGG	ban:BA1826. bar:GBAA1826. bat:BAS1691.
TIGR	BA_1826. GBAA_1826.
Phylogenomic databases
HOGENOM	HBG649927.
OMA	IESICEK.
Enzyme and pathway databases
BioCyc	BANT260799:BAS1691-MONOMER. BANT261594:GBAA1826-MONOMER.
BRENDA	2.3.1.180. 267517.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH2_BACAN

Accession

Primary (citable) accession number: Q81JF6
Secondary accession number(s): Q6I0C9, Q6KUA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)