Q81J97 (KPRS_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	BC_0055

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141108
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81J97 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 16414CD5645DA23A	FASTA	317	34,836
	10 20 30 40 50 60 MSTQYLNSNL KVFSLNSNKE LAEQIAKHIG VGLGKCSVDR FSDGEVQINI EESIRGCDVF 70 80 90 100 110 120 IIQSTSFPVN EHIMELLIMI DALKRASAKT INIVIPYYGY ARQDRKARSR EPITSKLVAN 130 140 150 160 170 180 LLETAGATRV ITLDLHAPQI QGFFDIPIDH LMGVPILSDY FETKGLKDIV IVSPDHGGVT 190 200 210 220 230 240 RARKMADRLK APIAIIDKRR PRPNVSEVMN IIGNIEGKTA ILIDDIIDTA GTITLAANAL 250 260 270 280 290 300 VENGASEVYA CCTHPVLSGP AIERIQNSNI KELVVTNSIV LPEEKKIDKV HELSVAPLIG 310 EAIIRVYEEE SVSVLFN

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP07153.1.
RefSeq	NP_829952.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81J97.
SMR	Q81J97. Positions 2-316.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81J97.
Proteomic databases
PRIDE	Q81J97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000032485; EBBACP00000031691; EBBACG00000032476.
GeneID	1202408.
GenomeReviews	Gene locus BC_0055 in contig AE016877_GR.
KEGG	bce:BC0055.
PATRIC	32595717. VBIBacCer54481_0072.
Phylogenomic databases
eggNOG	COG0462.
GeneTree	EBGT00050000003045.
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	Q81J97.
ProtClustDB	PRK01259.
Enzyme and pathway databases
BioCyc	BCER226900:BC_0055-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BACCR

Accession

Primary (citable) accession number: Q81J97

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program