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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 35ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:BC_0070
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816431 – 476tRNA(Ile)-lysidine synthaseAdd BLAST476

Interactioni

Protein-protein interaction databases

STRINGi226900.BC0070.

Structurei

3D structure databases

ProteinModelPortaliQ81J84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
KOiK04075.
OMAiNDISYPL.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81J84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTFVEKVD DFVKQHDVLK NDSTIVVGVS GGPDSLALLY YLLEKRAEKQ
60 70 80 90 100
LEIVVAHVDH MFRGDESYED LQFVQGLCEE LGIICETIRI NVSQYQQQYG
110 120 130 140 150
MNAQVAAREC RYAFLERIMK KYDARYVALG HHGDDQVETI LMRLVRGSTP
160 170 180 190 200
KGYAGIAVKR PFHNGYLIRP LLGVTKEEIV DYCNKLNLMP RIDPSNKKEV
210 220 230 240 250
YTRNRLRKYV LPHLKEENPQ MHEKFQKFSV QMQEDEAYLQ ELAFEKMNKV
260 270 280 290 300
ITKKSDKQIS LSIPAFESMS MPLQRRGIQL ILNYLYEYKI PSSLSSIHID
310 320 330 340 350
KLIEFFKRTQ PSGSLDFPGD LKIVRSYEEC SFRFKQEIVS PFSQDLLVPG
360 370 380 390 400
TITLLNGDKF VTEVSEDIPS NMNETVFVAK YNDISYPLRI RSRENGDRMS
410 420 430 440 450
IQGMNGTKKI KAIFIEAKVP KEKREEWPVV CDASGTIIWV PLLKRSAFAI
460 470
SKEMAKKDKY MIIHYKSKES SGRIMK
Length:476
Mass (Da):55,024
Last modified:June 1, 2003 - v1
Checksum:iAE339160226EC6BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP07166.1.
RefSeqiNP_829965.1. NC_004722.1.
WP_000655465.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP07166; AAP07166; BC_0070.
GeneIDi1202423.
KEGGibce:BC0070.
PATRICi32595747. VBIBacCer54481_0085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP07166.1.
RefSeqiNP_829965.1. NC_004722.1.
WP_000655465.1. NC_004722.1.

3D structure databases

ProteinModelPortaliQ81J84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226900.BC0070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP07166; AAP07166; BC_0070.
GeneIDi1202423.
KEGGibce:BC0070.
PATRICi32595747. VBIBacCer54481_0085.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
KOiK04075.
OMAiNDISYPL.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_BACCR
AccessioniPrimary (citable) accession number: Q81J84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.