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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei255ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:BC_0108
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195001 – 485Glutamate--tRNA ligaseAdd BLAST485

Proteomic databases

PRIDEiQ81J61.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi226900.BC0108.

Structurei

3D structure databases

ProteinModelPortaliQ81J61.
SMRiQ81J61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi252 – 256"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008. LUCA.
KOiK09698.
OMAiAYYAFDT.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81J61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKQVRVRYA PSPTGHLHIG NARTALFNYL FARHLDGKFI IRIEDTDVKR
60 70 80 90 100
NVAGGEESQL KYLKWLGMDW DEGVDVGGEF GPYRQTERLD IYKKLYEDLL
110 120 130 140 150
ERGLAYKCYM TEEELEAERE GQIARGETPR YACNHRELTE EQVKEFEAEG
160 170 180 190 200
RIPSIRFRVP ANRDYTFKDI VKDEVAFHSN DFGDFVIVKK DGIPTYNFAV
210 220 230 240 250
AVDDHLMEIT HVLRGDDHIS NTPKQMMIYE AFGWDIPQFG HMTLIVNESR
260 270 280 290 300
KKLSKRDESI IQFIEQYKEL GYLPEAIFNF IALLGWSPVG EEEIFSKDEF
310 320 330 340 350
IKMFDAARLS KSPALFDSQK LKWMNNQYMK KQDLDTVVEL SLPHLVKAGR
360 370 380 390 400
VGETLSEQDQ AWIRDVIALY HEQMSFGAEI VELSEMFFKD HVDYEEEGQE
410 420 430 440 450
VLNGEQVPEV LRAFAGQIEA LEVMEPAAIK AAIKAVQKET GHKGKNLFMP
460 470 480
IRVATTGQTH GPELPNAIAL LGKEKVLNRL QKVIG
Length:485
Mass (Da):55,704
Last modified:July 25, 2003 - v2
Checksum:i73CD0A8E3ACE0372
GO

Sequence cautioni

The sequence AAP07190 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP07190.1. Different initiation.
RefSeqiNP_829989.1. NC_004722.1.
WP_011109773.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP07190; AAP07190; BC_0108.
GeneIDi1202461.
KEGGibce:BC0108.
PATRICifig|226900.8.peg.110.

Similar proteinsi

Entry informationi

Entry nameiSYE_BACCR
AccessioniPrimary (citable) accession number: Q81J61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: September 27, 2017
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families