Reviewed, UniProtKB/Swiss-Prot Q81IU1 (DDLB_BACCR)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase B
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetaseB
    D-Ala-D-Ala ligase B

Gene names

Name:	ddlB
Ordered Locus Names:	BC_0257

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	D-alanine--D-alanine ligase B		PRO_0000177783
Regions
	Domain	140 – 345	206	ATP-grasp
	Nucleotide binding	173 – 228	56	ATP By similarity
Sites
	Metal binding	299	1	Magnesium or manganese 1 By similarity
	Metal binding	312	1	Magnesium or manganese 1 By similarity
	Metal binding	312	1	Magnesium or manganese 2 By similarity
	Metal binding	314	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81IU1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 885D9E7C8FDC9214	FASTA	361	40,073
	10 20 30 40 50 60 MTKIKLGLLY GGKSAEHQVS LQTALAAIKA LNQDKFEIHP IYITEQGQWV RGERIEGEVT 70 80 90 100 110 120 DVEALKMSGA ENAISPLSLS TEIIPSAASE ENAIDVIFPL LHGPNGEDGT VQGLLELMNI 130 140 150 160 170 180 PYVGNGVLAS SAGMDKVVMK NIFAEAGLKQ AKYASFIRSA WEKNREEAYS KVEDKLGYPC 190 200 210 220 230 240 FVKPANLGSS VGINKCKNRE ELEDAFVEAF QFDRKIIVEE NIVGREVEVG VLGNDEPKCS 250 260 270 280 290 300 VVGEIVPKKD FYDYKSKYID GDTALIIPAE MTEEESNVIK RDAIIAFQSL DGAGLTRADF 310 320 330 340 350 360 FLTKDGEVYI NEVNTMPGFT PFSMFPLLWQ HTGLPYPELI EELIRLAIER HEEKQKIKYT I

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP07326.1.
RefSeq	NP_830125.1.
3D structure databases
HSSP	HSSP built from PDB template 1IOW based on UniProtKB P07862.
ModBase	Search...
Genome annotation databases
GeneID	1202610.
GenomeReviews	Gene locus BC_0257 in contig AE016877_GR.
KEGG	bce:BC0257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81IU1.
Enzyme and pathway databases
BioCyc	BCER226900:BC_0257-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLB_BACCR

Accession

Primary (citable) accession number: Q81IU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)