Reviewed, UniProtKB/Swiss-Prot Q81IS8 (GCP_BACCR)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BC_0289

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303264
Sites
	Metal binding	120	1	Zinc Potential
	Metal binding	124	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81IS8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5A313BA2C7001BDB	FASTA	343	36,622
	10 20 30 40 50 60 MGEYIMEKNT IILGIETSCD ETAVAVVKNG TEIIANVVAS QIESHKRFGG VVPEIASRHH 70 80 90 100 110 120 VEEITVVLEE ALKEANITFD DIDAIAVTEG PGLVGALLIG VNAAKAVAFA HDIPLVGVHH 130 140 150 160 170 180 IAGHIYANRL VKEVQFPLLS LVVSGGHTEL VYMKEHGSFE VIGETRDDAA GEAYDKVART 190 200 210 220 230 240 LSMPYPGGPH IDRLAHEGEP TIDLPRAWLE PDSYDFSFSG LKSAVINTVH NAKQRGIEIA 250 260 270 280 290 300 PEDLAASFQE SVIDVLVTKA ARAAEAYNVK QLLLAGGVAA NKGLRARLEE EFAQKENIEL 310 320 330 340 IIPPLSLCTD NAAMIAAAGT IAYEQGKRAT LALNANPGLD IEA

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP07341.1.
RefSeq	NP_830140.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q81IS8.
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	1202642.
GenomeReviews	Gene locus BC_0289 in contig AE016877_GR.
KEGG	bce:BC0289.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81IS8.
OMA	CKRALKQ.
Enzyme and pathway databases
BioCyc	BCER226900:BC_0289-MON.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BACCR

Accession

Primary (citable) accession number: Q81IS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)