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Protein

N-succinyl-L-Arg/Lys racemase

Gene

BC_0371

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes efficient racemization of N-succinyl-L-Arg and N-succinyl-L-Lys, suggesting that these are physiological substrates of this enzyme. Has low activity with L-Asp-L-Lys, and even lower activity with L-Leu-L-Arg, L-Leu-L-Lys, N-succinyl-L-His and N-succinyl-L-Met (in vitro).1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

  1. KM=0.5 mM for N-succinyl-L-Arg1 Publication
  2. KM=3.0 mM for N-succinyl-L-Met1 Publication
  3. KM=11 mM for N-succinyl-L-His1 Publication
  4. KM=1.2 mM for L-Leu-L-Lys1 Publication
  5. KM=3.9 mM for L-Asp-L-Lys1 Publication
  6. KM=16 mM for L-Leu-L-Arg1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei26 – 261Substrate
    Binding sitei51 – 511Substrate
    Metal bindingi191 – 1911Magnesium1 Publication
    Metal bindingi218 – 2181Magnesium1 Publication
    Metal bindingi243 – 2431Magnesium1 Publication
    Binding sitei267 – 2671Substrate

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • racemase activity, acting on amino acids and derivatives Source: CACAO
    • racemase and epimerase activity Source: UniProtKB

    GO - Biological processi

    • peptide metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBCER226900:GJEU-373-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-succinyl-L-Arg/Lys racemase (EC:5.1.1.-)
    Alternative name(s):
    N-succinyl amino acid racemase
    Short name:
    NSAR
    Gene namesi
    Ordered Locus Names:BC_0371
    OrganismiBacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
    Taxonomic identifieri226900 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    Proteomesi
    • UP000001417 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369N-succinyl-L-Arg/Lys racemasePRO_0000429658Add
    BLAST

    Proteomic databases

    PRIDEiQ81IL5.

    Interactioni

    Protein-protein interaction databases

    STRINGi226900.BC0371.

    Structurei

    Secondary structure

    1
    369
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 2119Combined sources
    Beta strandi24 – 3815Combined sources
    Beta strandi43 – 486Combined sources
    Helixi52 – 554Combined sources
    Helixi59 – 6810Combined sources
    Helixi70 – 745Combined sources
    Helixi82 – 9211Combined sources
    Helixi97 – 11418Combined sources
    Helixi119 – 1224Combined sources
    Beta strandi135 – 1373Combined sources
    Helixi142 – 15413Combined sources
    Beta strandi159 – 1635Combined sources
    Helixi168 – 18215Combined sources
    Beta strandi186 – 1916Combined sources
    Turni193 – 1964Combined sources
    Helixi199 – 2079Combined sources
    Helixi210 – 2123Combined sources
    Helixi226 – 2349Combined sources
    Beta strandi240 – 2434Combined sources
    Helixi249 – 25810Combined sources
    Beta strandi262 – 2665Combined sources
    Helixi268 – 2714Combined sources
    Helixi274 – 28613Combined sources
    Beta strandi290 – 2934Combined sources
    Helixi300 – 31011Combined sources
    Beta strandi316 – 3194Combined sources
    Helixi323 – 3264Combined sources
    Helixi355 – 3617Combined sources
    Beta strandi362 – 3687Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P88X-ray2.40A/B/C/D/E/F/G/H1-369[»]
    2P8BX-ray1.70A1-369[»]
    2P8CX-ray2.00A1-369[»]
    ProteinModelPortaliQ81IL5.
    SMRiQ81IL5. Positions 1-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ81IL5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni161 – 1633Substrate binding
    Regioni191 – 1933Substrate binding
    Regioni295 – 2962Substrate binding
    Regioni320 – 3223Substrate binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105DTQ. Bacteria.
    COG4948. LUCA.
    OMAiHEEFPVT.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N-like.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N_dom.
    IPR001354. MR/MLE/MAL.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF13378. MR_MLE_C. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q81IL5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKITAIHLYA IRLPLRNPFV ISYGSYSDMP SIIVKMETDE GIIGYGEGVA
    60 70 80 90 100
    DDHVTGESWE STFHTLKHTL TPALIGQNPM NIEKIHDMMD NTIYGVPTAK
    110 120 130 140 150
    AAIDIACFDI MGKKLNQPVY QLIGGRYHEE FPVTHVLSIA DPENMAEEAA
    160 170 180 190 200
    SMIQKGYQSF KMKVGTNVKE DVKRIEAVRE RVGNDIAIRV DVNQGWKNSA
    210 220 230 240 250
    NTLTALRSLG HLNIDWIEQP VIADDIDAMA HIRSKTDLPL MIDEGLKSSR
    260 270 280 290 300
    EMRQIIKLEA ADKVNIKLMK CGGIYPAVKL AHQAEMAGIE CQVGSMVESS
    310 320 330 340 350
    VASSAGFHVA FSKKIITSVE LTGPLKFTKD IGNLHYDVPF IRLNEKPGLG
    360
    IEINEDTLQE LTVFQDIVR
    Length:369
    Mass (Da):40,949
    Last modified:June 1, 2003 - v1
    Checksum:i26D38191EC5D0450
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016877 Genomic DNA. Translation: AAP07411.1.
    RefSeqiNP_830210.1. NC_004722.1.
    WP_000704492.1. NC_004722.1.

    Genome annotation databases

    EnsemblBacteriaiAAP07411; AAP07411; BC_0371.
    GeneIDi1202724.
    KEGGibce:BC0371.
    PATRICi32596372. VBIBacCer54481_0343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016877 Genomic DNA. Translation: AAP07411.1.
    RefSeqiNP_830210.1. NC_004722.1.
    WP_000704492.1. NC_004722.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P88X-ray2.40A/B/C/D/E/F/G/H1-369[»]
    2P8BX-ray1.70A1-369[»]
    2P8CX-ray2.00A1-369[»]
    ProteinModelPortaliQ81IL5.
    SMRiQ81IL5. Positions 1-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi226900.BC0371.

    Proteomic databases

    PRIDEiQ81IL5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAP07411; AAP07411; BC_0371.
    GeneIDi1202724.
    KEGGibce:BC0371.
    PATRICi32596372. VBIBacCer54481_0343.

    Phylogenomic databases

    eggNOGiENOG4105DTQ. Bacteria.
    COG4948. LUCA.
    OMAiHEEFPVT.

    Enzyme and pathway databases

    BioCyciBCER226900:GJEU-373-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ81IL5.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N-like.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N_dom.
    IPR001354. MR/MLE/MAL.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF13378. MR_MLE_C. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNSAR_BACCR
    AccessioniPrimary (citable) accession number: Q81IL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 9, 2014
    Last sequence update: June 1, 2003
    Last modified: September 7, 2016
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Part of a large, functionally divergent protein family. Protein modeling and substrate docking was used to predict the substrate specificity, prior to biochemical analysis.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.