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Q81IH1 (TOP3_BACCR) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:BC_0417
OrganismBacillus cereus (strain ATCC 14579 / DSM 31) [Reference proteome] [HAMAP]
Taxonomic identifier226900 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286361

Regions

Domain3 – 136134Toprim
Region187 – 1926Interaction with DNA By similarity

Sites

Active site3101O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium; catalytic By similarity
Metal binding1051Magnesium; catalytic By similarity
Site611Interaction with DNA By similarity
Site1681Interaction with DNA By similarity
Site1761Interaction with DNA By similarity
Site3121Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q81IH1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: DDF62B294E1BB655

FASTA72982,891
        10         20         30         40         50         60 
MSKSVVIAEK PSVARDIARV LKCDKKGNGY LEGSKYIVTW ALGHLVTLAD PESYDVKYKK 

        70         80         90        100        110        120 
WNLEDLPMLP ERLKLTVIKQ TGKQFNAVKS QLLRKDVNEI IVATDAGREG ELVARWIIDK 

       130        140        150        160        170        180 
VKLNKPIKRL WISSVTDKAI KDGFANLKPG KAYDNLYASA VARSEADWYI GLNATRALTT 

       190        200        210        220        230        240 
RFNAQLNCGR VQTPTVAMIA SREDEIKNFK AQTYYGIEAQ TMEKLKLTWQ DANGNSRSFN 

       250        260        270        280        290        300 
KEKIDGIVKR LDKQNATVVE IDKKQKKSFS PGLYDLTELQ RDANKKFGYS AKETLNIMQK 

       310        320        330        340        350        360 
LYEQHKVLTY PRTDSRYISS DIVGTLPERL KACGVGEYRP FAHKVLQKPI KPNKSFVDDS 

       370        380        390        400        410        420 
KVSDHHAIIP TEGYVNFSAF TDKERKIYDL VVKRFLAVLF PAFEYEQLTL RTKVGNETFI 

       430        440        450        460        470        480 
ARGKTILHAG WKEVYENRFE DDDVTDDVKE QLLPHIEKGD TLAVKLIMQT SGQTKAPARF 

       490        500        510        520        530        540 
NEATLLSAME NPTKYMDTQN KQLADTLKST GGLGTVATRA DIIDKLFNSF LIEKRGKDIH 

       550        560        570        580        590        600 
ITSKGRQLLD LVPEELKSPT LTGEWEQKLE AIAKGKLKKE VFISEMKNYT KEIVSEIKSS 

       610        620        630        640        650        660 
DKKYKHDNIS TKSCPDCGKP MLEVNGKKGK MLVCQDRECG HRKNVSRTTN ARCPQCKKKL 

       670        680        690        700        710        720 
ELRGEGAGQI FACKCGYREK LSTFQERRKK ESGNKADKRD VQKYMKQQNK EEEPLNNPFA 


EALKKLKFD 

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References

[1]"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis."
Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. expand/collapse author list , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.
Nature 423:87-91(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016877 Genomic DNA. Translation: AAP07457.1.
RefSeqNP_830256.1. NC_004722.1.

3D structure databases

ProteinModelPortalQ81IH1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226900.BC0417.

Proteomic databases

PRIDEQ81IH1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP07457; AAP07457; BC_0417.
GeneID1202770.
KEGGbce:BC0417.
PATRIC32596460. VBIBacCer54481_0387.

Phylogenomic databases

eggNOGCOG0551.
KOK03169.
OMATKHDEYF.
OrthoDBEOG60CWM6.

Enzyme and pathway databases

BioCycBCER226900:GJEU-419-MONOMER.
BRENDA5.99.1.2. 648.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_BACCR
AccessionPrimary (citable) accession number: Q81IH1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families