Reviewed, UniProtKB/Swiss-Prot Q81IA1 (UGTP_BACCR)

Names and origin

Protein names

Recommended name:
    Processive diacylglycerol glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    UDP glucosyltransferase
    UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferase
    Monoglucosyldiacylglycerol synthase
      Short name=MGlcDAG synthase
    Diglucosyldiacylglycerol synthase
    Beta-gentiobiosyldiacylglycerol synthase
    DGlcDAG synthase
    Glc2-DAG synthase
    Triglucosyldiacylglycerol synthase
      Short name=TGlcDAG synthase

Gene names

Name:	ugtP
Ordered Locus Names:	BC_0493

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is a processive glucosyltransferase involved in the synthesis of both bilayer- and non-bilayer-forming membrane glycolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of mono-, di- and triglucosyldiacylglycerol. Diglucosyldiacylglycerol (Glc2-DAG) is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA) By similarity.
Catalytic activity	UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol.
Pathway	Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. HAMAP MF_01280
Sequence similarities	Belongs to the glycosyltransferase 1 family. UgtP subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW glycolipid biosynthetic process Inferred from electronic annotation. Source: HAMAP lipid glycosylation Inferred from electronic annotation. Source: InterPro
Molecular function	UDP-glucosyltransferase activity Inferred from electronic annotation. Source: HAMAP carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Processive diacylglycerol glucosyltransferase HAMAP MF_01280		PRO_0000308449

Sequences

	Sequence		Length	Mass (Da)
	Q81IA1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 978D3C6F8D582887	FASTA	388	43,779
	10 20 30 40 50 60 MIKNPKVLIL TAHYGNGHVQ VAKTLEQTFR QKGIEDVIVC DLFGESHPFI TDITKYLYLK 70 80 90 100 110 120 SYTIGKELYR LFYYGVEKIY DKKIASWYAN FGRKRLKTLL QVEKPDIVIN TFPIIAVPEL 130 140 150 160 170 180 KKQTGISIPV YNVLTDFCVH KIWIHREVDR YFVATDHVKE LMVDIGVPAE QIVETGIPIR 190 200 210 220 230 240 SSFELKVNPE IIYTKYQLCK NKKILLIVAG AHGVLGNVKE LCQSFMSVPN LQVVVVCGKN 250 260 270 280 290 300 EALKQDLLSL QKQNSDALKV FGYVENIDEL FRVTSCMITK PGGITLSEAA ALQVPVILYK 310 320 330 340 350 360 PVPGQENENA MYFERKGAAV VIRDDSEVFA KTEALLQDDV KLLQMKEAMK SIYLPEPAGH 370 380 IVDAILAENH AEPRHIPIKS PALAQSFT

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP07531.1.
RefSeq	NP_830330.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Genome annotation databases
GeneID	1202846.
GenomeReviews	Gene locus BC_0493 in contig AE016877_GR.
KEGG	bce:BC0493.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81IA1.
OMA	Q81IA1. PDVIICT.
Enzyme and pathway databases
BioCyc	BCER226900:BC_0493-MON.
Family and domain databases
HAMAP	MF_01280. [Tree]
InterPro	IPR007235. Glyco_trans_28_C. IPR009695. MGDG_synth. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGTP_BACCR

Accession

Primary (citable) accession number: Q81IA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)