Reviewed, UniProtKB/Swiss-Prot Q81GN7 (HEMH2_BACCR)

Names and origin

Protein names

Recommended name:
    Ferrochelatase 2
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase 2
    Heme synthetase 2

Gene names

Name:	hemH2
Ordered Locus Names:	BC_1154

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ferrochelatase 2 HAMAP MF_00323		PRO_0000175107
Sites
	Metal binding	181	1	Iron By similarity
	Metal binding	262	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81GN7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 483009F2A5B8C599	FASTA	319	36,230
	10 20 30 40 50 60 MKKKKIGLLV MAYGTPESLD DVEAYYTHIR HGRKPSEEAL EDLIGRYKAI GGISPLAKIT 70 80 90 100 110 120 KEQAHKLTDS MNNIFTEYEF TCYLGLKHTA PFIEDAVEEM KRNGIEQTIS IVLAPHYSTF 130 140 150 160 170 180 SIKAYNDRAI RLSKEIGGPV IESIEQWYDE PKFISYWADQ IKETFTEIDD KEKAVVIFSA 190 200 210 220 230 240 HSLPEKIIAA GDPYVEQLKH TADLIAEAAN IQNYTIGWQS AGNTPDPWIG PDVQDLTKDL 250 260 270 280 290 300 YEEHGYESFI YCPVGFVAEH LEVLYDNDYE CKVVTDELNA KYFRPNMPNA QSAFIDCLAE 310 IVSKKVKEIV DKDLVLNNN

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP08141.1.
RefSeq	NP_830940.1.
3D structure databases
HSSP	HSSP built from PDB template 1DOZ based on UniProtKB P32396.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81GN7.
Genome annotation databases
GeneID	1203503.
GenomeReviews	Gene locus BC_1154 in contig AE016877_GR.
KEGG	bce:BC1154.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81GN7.
OMA	FISYWAD.
Enzyme and pathway databases
BioCyc	BCER226900:BC_1154-MON.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH2_BACCR

Accession

Primary (citable) accession number: Q81GN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)