Q81GJ7 (PRPE_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BC_1202

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297697

Sequences

	Sequence		Length	Mass (Da)
	Q81GJ7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1A1E92BF339C82F8	FASTA	246	28,192
	10 20 30 40 50 60 MKYDIIGDIH GCFQEFQNLT EKLGYNWSSG LPVHPDQRKL AFVGDITDRG PHSLRMIEIV 70 80 90 100 110 120 WELVIHKKEA YYAPGNHCNK LYRFFLGRNV TVAHGLETTV AEYEALPSHK QNIIKEKFIT 130 140 150 160 170 180 LYEQSPLYHI LDEKRVIVCH AGIRQDYIGR RDKKVQTFVL YGDITGEKHA DGSPVRRDWA 190 200 210 220 230 240 QEYKGQAWIV YGHTPVAEPR FVNQTVNIDT GAVFGGKLTG LRYPEMETIS VPSSLPFVAE KFRPIS

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08187.1.
RefSeq	NP_830986.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81GJ7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81GJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000031768; EBBACP00000030974; EBBACG00000031759.
GeneID	1203551.
GenomeReviews	Gene locus BC_1202 in contig AE016877_GR.
KEGG	bce:BC1202.
PATRIC	32598119. VBIBacCer54481_1171.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	EBGT00050000002430.
HOGENOM	HBG724457.
OMA	YVPGNHC.
PhylomeDB	Q81GJ7.
ProtClustDB	PRK13625.
Enzyme and pathway databases
BioCyc	BCER226900:BC_1202-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
KO	K01090.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACCR

Accession

Primary (citable) accession number: Q81GJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program