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Q81GI3

- FABI_BACCR

UniProt

Q81GI3 - FABI_BACCR

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygen1 Publication
    Binding sitei94 – 941NAD; via carbonyl oxygen1 Publication
    Binding sitei97 – 971Substrate; via amide nitrogen and carbonyl oxygen
    Active sitei147 – 1471Proton acceptorBy similarity
    Active sitei157 – 1571Proton acceptor
    Binding sitei164 – 1641NAD1 Publication
    Sitei205 – 2051Involved in acyl-ACP bindingBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NAD1 Publication
    Nucleotide bindingi66 – 672NAD1 Publication
    Nucleotide bindingi193 – 1975NAD1 Publication

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
    2. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB
    2. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBCER226900:GJEU-1216-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Ordered Locus Names:BC_1216
    OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
    Taxonomic identifieri226900 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000001417: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000407976Add
    BLAST

    Proteomic databases

    PRIDEiQ81GI3.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi226900.BC1216.

    Structurei

    Secondary structure

    1
    256
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 125
    Helixi20 – 2910
    Turni30 – 323
    Beta strandi34 – 396
    Helixi45 – 5410
    Beta strandi55 – 584
    Beta strandi62 – 643
    Helixi70 – 8415
    Beta strandi89 – 924
    Helixi99 – 1013
    Beta strandi102 – 1043
    Helixi106 – 1083
    Helixi111 – 12111
    Helixi123 – 13311
    Beta strandi140 – 1467
    Helixi148 – 1503
    Turni155 – 1573
    Helixi158 – 17821
    Helixi179 – 1813
    Beta strandi183 – 1908
    Helixi196 – 1983
    Helixi204 – 21411
    Helixi223 – 23412
    Helixi236 – 2383
    Beta strandi245 – 2495
    Helixi252 – 2543

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OJEX-ray3.02A1-256[»]
    3OJFX-ray2.20A/B/C/D1-256[»]
    ProteinModelPortaliQ81GI3.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ81GI3.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiTGEIHHV.
    OrthoDBiEOG6HF644.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    Q81GI3-1 [UniParc]FASTAAdd to Basket

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    MELLQGKTFV VMGVANQRSI AWGIARSLHN AGAKLIFTYA GERLERNVRE    50
    LADTLEGQES LVLPCDVTND EELTACFETI KQEVGTIHGV AHCIAFANRD 100
    DLKGEFVDTS RDGFLLAQNI SAFSLTAVAR EAKKVMTEGG NILTLTYLGG 150
    ERVVKNYNVM GVAKASLEAS VKYLANDLGQ HGIRVNAISA GPIRTLSAKG 200
    VGDFNSILRE IEERAPLRRT TTQEEVGDTA VFLFSDLARG VTGENIHVDS 250
    GYHILG 256
    Length:256
    Mass (Da):27,740
    Last modified:June 1, 2003 - v1
    Checksum:i66780350F497CA31
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016877 Genomic DNA. Translation: AAP08201.1.
    RefSeqiNP_831000.1. NC_004722.1.

    Genome annotation databases

    EnsemblBacteriaiAAP08201; AAP08201; BC_1216.
    GeneIDi1203565.
    KEGGibce:BC1216.
    PATRICi32598149. VBIBacCer54481_1186.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016877 Genomic DNA. Translation: AAP08201.1 .
    RefSeqi NP_831000.1. NC_004722.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3OJE X-ray 3.02 A 1-256 [» ]
    3OJF X-ray 2.20 A/B/C/D 1-256 [» ]
    ProteinModelPortali Q81GI3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226900.BC1216.

    Proteomic databases

    PRIDEi Q81GI3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP08201 ; AAP08201 ; BC_1216 .
    GeneIDi 1203565.
    KEGGi bce:BC1216.
    PATRICi 32598149. VBIBacCer54481_1186.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi TGEIHHV.
    OrthoDBi EOG6HF644.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci BCER226900:GJEU-1216-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q81GI3.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSi PR00081. GDHRDH.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14579 / DSM 31.
    2. "Dimeric and tetrameric forms of enoyl-acyl carrier protein reductase from Bacillus cereus."
      Kim S.J., Ha B.H., Kim K.H., Hong S.K., Shin K.J., Suh S.W., Kim E.E.
      Biochem. Biophys. Res. Commun. 400:517-522(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH NAD AND INHIBITOR, SUBUNIT.

    Entry informationi

    Entry nameiFABI_BACCR
    AccessioniPrimary (citable) accession number: Q81GI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3