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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygen1 Publication1
Binding sitei94NAD; via carbonyl oxygen1 Publication1
Binding sitei97Substrate; via amide nitrogen and carbonyl oxygen1
Active sitei147Proton acceptorBy similarity1
Active sitei157Proton acceptor1
Binding sitei164NAD1 Publication1
Sitei205Involved in acyl-ACP bindingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NAD1 Publication2
Nucleotide bindingi66 – 67NAD1 Publication2
Nucleotide bindingi193 – 197NAD1 Publication5

GO - Molecular functioni

GO - Biological processi

  • fatty acid elongation Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi1.3.1.10. 648.
1.3.1.9. 648.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:BC_1216
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001417 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004079761 – 256Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST256

Proteomic databases

PRIDEiQ81GI3.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi226900.BC1216.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Helixi20 – 29Combined sources10
Turni30 – 32Combined sources3
Beta strandi34 – 39Combined sources6
Helixi45 – 54Combined sources10
Beta strandi55 – 58Combined sources4
Beta strandi62 – 64Combined sources3
Helixi70 – 84Combined sources15
Beta strandi89 – 92Combined sources4
Helixi99 – 101Combined sources3
Beta strandi102 – 104Combined sources3
Helixi106 – 108Combined sources3
Helixi111 – 121Combined sources11
Helixi123 – 133Combined sources11
Beta strandi140 – 146Combined sources7
Helixi148 – 150Combined sources3
Turni155 – 157Combined sources3
Helixi158 – 178Combined sources21
Helixi179 – 181Combined sources3
Beta strandi183 – 190Combined sources8
Helixi196 – 198Combined sources3
Helixi204 – 214Combined sources11
Helixi223 – 234Combined sources12
Helixi236 – 238Combined sources3
Beta strandi245 – 249Combined sources5
Helixi252 – 254Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OJEX-ray3.02A1-256[»]
3OJFX-ray2.20A/B/C/D1-256[»]
ProteinModelPortaliQ81GI3.
SMRiQ81GI3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81GI3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623. LUCA.
KOiK00208.
OMAiAGYCING.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q81GI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLQGKTFV VMGVANQRSI AWGIARSLHN AGAKLIFTYA GERLERNVRE
60 70 80 90 100
LADTLEGQES LVLPCDVTND EELTACFETI KQEVGTIHGV AHCIAFANRD
110 120 130 140 150
DLKGEFVDTS RDGFLLAQNI SAFSLTAVAR EAKKVMTEGG NILTLTYLGG
160 170 180 190 200
ERVVKNYNVM GVAKASLEAS VKYLANDLGQ HGIRVNAISA GPIRTLSAKG
210 220 230 240 250
VGDFNSILRE IEERAPLRRT TTQEEVGDTA VFLFSDLARG VTGENIHVDS

GYHILG
Length:256
Mass (Da):27,740
Last modified:June 1, 2003 - v1
Checksum:i66780350F497CA31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP08201.1.
RefSeqiNP_831000.1. NC_004722.1.
WP_000421886.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP08201; AAP08201; BC_1216.
GeneIDi1203565.
KEGGibce:BC1216.
PATRICi32598149. VBIBacCer54481_1186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP08201.1.
RefSeqiNP_831000.1. NC_004722.1.
WP_000421886.1. NC_004722.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OJEX-ray3.02A1-256[»]
3OJFX-ray2.20A/B/C/D1-256[»]
ProteinModelPortaliQ81GI3.
SMRiQ81GI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226900.BC1216.

Proteomic databases

PRIDEiQ81GI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP08201; AAP08201; BC_1216.
GeneIDi1203565.
KEGGibce:BC1216.
PATRICi32598149. VBIBacCer54481_1186.

Phylogenomic databases

eggNOGiCOG0623. LUCA.
KOiK00208.
OMAiAGYCING.

Enzyme and pathway databases

UniPathwayiUPA00094.
BRENDAi1.3.1.10. 648.
1.3.1.9. 648.

Miscellaneous databases

EvolutionaryTraceiQ81GI3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABI_BACCR
AccessioniPrimary (citable) accession number: Q81GI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.