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Q81GI3

- FABI_BACCR

UniProt

Q81GI3 - FABI_BACCR

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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen1 Publication
Binding sitei94 – 941NAD; via carbonyl oxygen1 Publication
Binding sitei97 – 971Substrate; via amide nitrogen and carbonyl oxygen
Active sitei147 – 1471Proton acceptorBy similarity
Active sitei157 – 1571Proton acceptor
Binding sitei164 – 1641NAD1 Publication
Sitei205 – 2051Involved in acyl-ACP bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD1 Publication
Nucleotide bindingi66 – 672NAD1 Publication
Nucleotide bindingi193 – 1975NAD1 Publication

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
  2. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
  2. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCER226900:GJEU-1216-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:BC_1216
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001417: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000407976Add
BLAST

Proteomic databases

PRIDEiQ81GI3.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi226900.BC1216.

Structurei

Secondary structure

1
256
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 125Combined sources
Helixi20 – 2910Combined sources
Turni30 – 323Combined sources
Beta strandi34 – 396Combined sources
Helixi45 – 5410Combined sources
Beta strandi55 – 584Combined sources
Beta strandi62 – 643Combined sources
Helixi70 – 8415Combined sources
Beta strandi89 – 924Combined sources
Helixi99 – 1013Combined sources
Beta strandi102 – 1043Combined sources
Helixi106 – 1083Combined sources
Helixi111 – 12111Combined sources
Helixi123 – 13311Combined sources
Beta strandi140 – 1467Combined sources
Helixi148 – 1503Combined sources
Turni155 – 1573Combined sources
Helixi158 – 17821Combined sources
Helixi179 – 1813Combined sources
Beta strandi183 – 1908Combined sources
Helixi196 – 1983Combined sources
Helixi204 – 21411Combined sources
Helixi223 – 23412Combined sources
Helixi236 – 2383Combined sources
Beta strandi245 – 2495Combined sources
Helixi252 – 2543Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OJEX-ray3.02A1-256[»]
3OJFX-ray2.20A/B/C/D1-256[»]
ProteinModelPortaliQ81GI3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81GI3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiTGEIHHV.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Q81GI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELLQGKTFV VMGVANQRSI AWGIARSLHN AGAKLIFTYA GERLERNVRE
60 70 80 90 100
LADTLEGQES LVLPCDVTND EELTACFETI KQEVGTIHGV AHCIAFANRD
110 120 130 140 150
DLKGEFVDTS RDGFLLAQNI SAFSLTAVAR EAKKVMTEGG NILTLTYLGG
160 170 180 190 200
ERVVKNYNVM GVAKASLEAS VKYLANDLGQ HGIRVNAISA GPIRTLSAKG
210 220 230 240 250
VGDFNSILRE IEERAPLRRT TTQEEVGDTA VFLFSDLARG VTGENIHVDS

GYHILG
Length:256
Mass (Da):27,740
Last modified:June 1, 2003 - v1
Checksum:i66780350F497CA31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP08201.1.
RefSeqiNP_831000.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP08201; AAP08201; BC_1216.
GeneIDi1203565.
KEGGibce:BC1216.
PATRICi32598149. VBIBacCer54481_1186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP08201.1 .
RefSeqi NP_831000.1. NC_004722.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3OJE X-ray 3.02 A 1-256 [» ]
3OJF X-ray 2.20 A/B/C/D 1-256 [» ]
ProteinModelPortali Q81GI3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226900.BC1216.

Proteomic databases

PRIDEi Q81GI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP08201 ; AAP08201 ; BC_1216 .
GeneIDi 1203565.
KEGGi bce:BC1216.
PATRICi 32598149. VBIBacCer54481_1186.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi TGEIHHV.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci BCER226900:GJEU-1216-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q81GI3.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14579 / DSM 31.
  2. "Dimeric and tetrameric forms of enoyl-acyl carrier protein reductase from Bacillus cereus."
    Kim S.J., Ha B.H., Kim K.H., Hong S.K., Shin K.J., Suh S.W., Kim E.E.
    Biochem. Biophys. Res. Commun. 400:517-522(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH NAD AND INHIBITOR, SUBUNIT.

Entry informationi

Entry nameiFABI_BACCR
AccessioniPrimary (citable) accession number: Q81GI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3