Reviewed, UniProtKB/Swiss-Prot Q81GF2 (ODO1_BACCR)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	BC_1252

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	955 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity. HAMAP MF_01169
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2. HAMAP MF_01169
Cofactor	Thiamine pyrophosphate By similarity. HAMAP MF_01169
Subunit structure	Homodimer By similarity. HAMAP MF_01169
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 955	955	2-oxoglutarate dehydrogenase E1 component HAMAP MF_01169		PRO_0000162163

Sequences

	Sequence		Length	Mass (Da)
	Q81GF2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 99F267C26CA28433	FASTA	955	106,412
	10 20 30 40 50 60 MTRKNTTTNP WAKFHGPNLG YVIEQYDLYV TGAGSVDPEL QELFEIFGAP SFQDDVVTGD 70 80 90 100 110 120 NTATHFSPQN TGNIEKILKV VQLVEQIRSF GHTLAHINPM EDAANGQSLL EKAMSELSDA 130 140 150 160 170 180 DLKAIPAKTV WQDAPEGIHT ALDVIHRLKD VYTKSLAYEF SHIQDSEERA WLHQMVESNS 190 200 210 220 230 240 LRQPLSNKKR TALLKRLTAV EGFEQFLHKT FVGQKRFSIE GVDMLVPVLD EIVLEGAKNG 250 260 270 280 290 300 VEDVMIGMAH RGRLSVLAHV LEKPYSHMFA EFKHAKIEGA VANSGWTGDV KYHLGREQVV 310 320 330 340 350 360 SNEEVSTRVT LANNPSHLEF VNPVVEGFAR AAQENRKKSG LPDQDTSKSF VILVHGDAAF 370 380 390 400 410 420 PGQGIVSETL NLSRLNAYQT GGTIHVIANN AVGFTTDSYD SRSTKYSSDL AKGFDIPIVH 430 440 450 460 470 480 VNADDPEACL AAANLAIQYR MLFKKDFLID LIGYRRYGHN EMDDPAVTQP QVYKKIKNHP 490 500 510 520 530 540 TVRAIYADQL QAAGVLNADE VETITQFTQE QLKSDYAQVP PADTSDATIH VKVPDVVAKG 550 560 570 580 590 600 IQPIDTGVEI DSLRAINEGL LSWPEGFNVY PKVKKILERR KDALEENGKI EWALAESLAF 610 620 630 640 650 660 ASILQEGTPI RLTGQDSQRG TFAHRHIVLH DTDTNETYSP LHRLPNINAS FSVHNSPLSE 670 680 690 700 710 720 AAVVGYEYGY NVFAPETLVM WEAQYGDFSN TAQALFDQYV SAGRAKWGQK SGLVLLLPHG 730 740 750 760 770 780 YEGQGPEHSS ARPERFLQLA AENNWTVANL TSAAQYFHIL RRQASILGTE AVRPLVLMTP 790 800 810 820 830 840 KSLLRHPLTL STASQLSEGR FQPALEQENL GMKPNKVKRL VLSTGKMAID LAAEIESGKH 850 860 870 880 890 900 EYNLDEVHVV RIEQLYPFPA EKVQSIIKRF KNLEEIIWVQ EEPRNMGAWH YMAPILFELA 910 920 930 940 950 GDKVKTGYIG RPDRSSPSGG DPFAHKAEQE LIVAHALDVK YNFRQDKQEI EVYSN

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08237.1.
RefSeq	NP_831036.1.
3D structure databases
SMR	Q81GF2. Positions 75-940.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81GF2.
Genome annotation databases
GeneID	1203601.
GenomeReviews	Gene locus BC_1252 in contig AE016877_GR.
KEGG	bce:BC1252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0567.
HOGENOM	HBG289950.
OMA	EGDEPAF.
Enzyme and pathway databases
BioCyc	BCER226900:BC_1252-MONOMER.
Family and domain databases
HAMAP	MF_01169. SucA_OdhA. [Tree]
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_BACCR

Accession

Primary (citable) accession number: Q81GF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)