Q81FQ4 (NDK_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase
Short name=NDK
Short name=NDP kinase
EC=2.7.4.6

Alternative name(s):
Nucleoside-2-P kinase

Gene names

Name:	ndk
Ordered Locus Names:	BC_1515

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Reference proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate By similarity. HAMAP-Rule MF_00451
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. HAMAP-Rule MF_00451
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CTP biosynthetic process Inferred from electronic annotation. Source: HAMAP GTP biosynthetic process Inferred from electronic annotation. Source: HAMAP UTP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Nucleoside diphosphate kinase HAMAP-Rule MF_00451		PRO_0000136940
Sites
	Active site	115	1	Pros-phosphohistidine intermediate By similarity
	Binding site	9	1	ATP By similarity
	Binding site	57	1	ATP By similarity
	Binding site	85	1	ATP By similarity
	Binding site	91	1	ATP By similarity
	Binding site	102	1	ATP By similarity
	Binding site	112	1	ATP By similarity
Amino acid modifications
	Modified residue	91	1	Phosphothreonine By similarity
	Modified residue	122	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81FQ4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: BEF6D21EC54B354F	FASTA	148	16,642
	10 20 30 40 50 60 MEKTFLMVKP DGVQRAFIGE IVARFEKKGF QLVGAKLMQV TPEIAGQHYA EHKEKPFFGE 70 80 90 100 110 120 LVDFITSGPV FAMVWQGEGV VDTARNMMGK TRPHEAAPGT IRGDFGVTVA KNIIHGSDSL 130 140 ESAEREIAIF FKEEELVDYS KLMNEWIY

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08495.1.
RefSeq	NP_831294.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81FQ4.
SMR	Q81FQ4. Positions 2-137.
ModBase	Search...
Protein-protein interaction databases
STRING	226900.BC1515.
Proteomic databases
PRIDE	Q81FQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP08495; AAP08495; BC_1515.
GeneID	1203864.
KEGG	bce:BC1515.
PATRIC	32598766. VBIBacCer54481_1492.
Phylogenomic databases
eggNOG	COG0105.
KO	K00940.
OMA	FRVVAMK.
ProtClustDB	PRK00668.
Enzyme and pathway databases
BioCyc	BCER226900:GJEU-1515-MONOMER.
Family and domain databases
Gene3D	3.30.70.141. 1 hit.
HAMAP	MF_00451. NDP_kinase.
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
PANTHER	PTHR11349. PTHR11349. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK_BACCR

Accession

Primary (citable) accession number: Q81FQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program