Q81F85 (FUMC_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	BC_1712

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161253
Regions
	Region	127 – 130	4	B site By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81F85 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D87CF7C737840676	FASTA	462	50,347
	10 20 30 40 50 60 MEYRIERDTL GEIKVPADKL WAAQTQRSKE NFPIGTEQMP LEIVKAFAIL KKSAALSNQK 70 80 90 100 110 120 LGKLSEEKAE AIVEAADEVI AGKWNEHFPL VVWQTGSGTQ SNMNVNEVIA NRGNQILKEK 130 140 150 160 170 180 GSDVHIHPND DVNMSQSSND TFPTALHVAC VIAVENHVLP AITKLKETLV EKVTAFEHII 190 200 210 220 230 240 KIGRTHLQDA TPLTLGQEIS GWHRMLEKTE RMIAESNTYM KELAIGGTAV GTGINAHPKF 250 260 270 280 290 300 GEMVSEEISQ FTGKQFVSAP NKFHALTSHD EVVYTHGALK ALAADLMKIA NDVRWLASGP 310 320 330 340 350 360 RSGLGEIIIP ANEPGSSIMP GKVNPTQSEA LTMVVAQVMG NDATIGFAAS QGNFELNVFK 370 380 390 400 410 420 PVIAYNFLQS AHLLADAIVS FNDNCAVGIE ADEEVINENV NRSLMLVTAL NPHIGYENAA 430 440 450 460 KIAKHAHKEG LTLKEAALQS GLLTEEQFDE IVDPKKMIAP KE

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08688.1.
RefSeq	NP_831487.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81F85.
SMR	Q81F85. Positions 4-458.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81F85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000034812; EBBACP00000034018; EBBACG00000034803.
GeneID	1204061.
GenomeReviews	Gene locus BC_1712 in contig AE016877_GR.
KEGG	bce:BC1712.
PATRIC	32599183. VBIBacCer54481_1700.
Phylogenomic databases
eggNOG	COG0114.
GeneTree	EBGT00070000031773.
HOGENOM	HBG284369.
OMA	RIEKDTM.
PhylomeDB	Q81F85.
ProtClustDB	PRK00485.
Enzyme and pathway databases
BioCyc	BCER226900:BC_1712-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. FumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_BACCR

Accession

Primary (citable) accession number: Q81F85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program