Q81EP4 (LDH1_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 1
Short name=L-LDH 1
EC=1.1.1.27

Gene names

Name:	ldh1
Ordered Locus Names:	BC_1924

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	L-lactate dehydrogenase 1 HAMAP MF_00488		PRO_0000168321
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	123	1	NAD or substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81EP4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 19DC3B1C2083AB2B	FASTA	314	34,789
	10 20 30 40 50 60 MKKGINRVVL VGTGAVGCSY AYCMINQAVA EEFVLVDVNE AKAEGEAMDL SHAVPFAPAP 70 80 90 100 110 120 TRVWKGSYED CKDADLVVIT AGLPQKPGET RLDLVEKNAK IFKQIVRSIM DSGFDGIFLI 130 140 150 160 170 180 ATNPVDILTY VTWKESGLPK ERVIGSGTTL DSARFRYMLG EYFDIGPHNI HAYIIGEHGD 190 200 210 220 230 240 TELPVWSHVS VGIQKLQTLL EKDNTYNQED LDKIFINVRD AAYHIIERKG ATYYGIGMSL 250 260 270 280 290 300 LRVTKAILND ENSVLTVSAY LEGQYGQKDV YIGVPAVLNR GGVREILEVE LSEDEELKFD 310 HSVQVLKETM APVL

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08895.1.
RefSeq	NP_831694.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81EP4.
SMR	Q81EP4. Positions 4-311.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81EP4.
Proteomic databases
PRIDE	Q81EP4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000033719; EBBACP00000032925; EBBACG00000033710.
GeneID	1204273.
GenomeReviews	Gene locus BC_1924 in contig AE016877_GR.
KEGG	bce:BC1924.
PATRIC	32599637. VBIBacCer54481_1927.
Phylogenomic databases
eggNOG	COG0039.
GeneTree	EBGT00050000001153.
HOGENOM	HBG566126.
OMA	IHPLSCH.
PhylomeDB	Q81EP4.
ProtClustDB	PRK00066.
Enzyme and pathway databases
BioCyc	BCER226900:BC_1924-MONOMER.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_BACCR

Accession

Primary (citable) accession number: Q81EP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program