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Protein

L-lactate dehydrogenase 1

Gene

ldh1

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2), L-lactate dehydrogenase 3 (ldh3)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADUniRule annotation1
Binding sitei42NADUniRule annotation1
Binding sitei68NADUniRule annotation1
Binding sitei85SubstrateUniRule annotation1
Binding sitei91SubstrateUniRule annotation1
Binding sitei146NADUniRule annotation1
Binding sitei156Allosteric activatorUniRule annotation1
Binding sitei171Allosteric activatorUniRule annotation1
Active sitei178Proton acceptorUniRule annotation1
Binding sitei232SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 16NADUniRule annotation2
Nucleotide bindingi82 – 83NADUniRule annotation2
Nucleotide bindingi121 – 123NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1UniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDH 1UniRule annotation
Gene namesi
Name:ldh1UniRule annotation
Ordered Locus Names:BC_1924
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683211 – 314L-lactate dehydrogenase 1Add BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ81EP4

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi226900.BC1924

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi15 – 26Combined sources12
Beta strandi31 – 36Combined sources6
Helixi40 – 51Combined sources12
Helixi52 – 56Combined sources5
Beta strandi57 – 59Combined sources3
Beta strandi62 – 65Combined sources4
Helixi68 – 71Combined sources4
Beta strandi75 – 79Combined sources5
Helixi91 – 111Combined sources21
Beta strandi116 – 120Combined sources5
Beta strandi122 – 124Combined sources3
Helixi125 – 136Combined sources12
Helixi140 – 142Combined sources3
Beta strandi143 – 145Combined sources3
Helixi149 – 163Combined sources15
Helixi167 – 169Combined sources3
Beta strandi174 – 179Combined sources6
Helixi186 – 188Combined sources3
Helixi196 – 201Combined sources6
Beta strandi202 – 204Combined sources3
Helixi208 – 219Combined sources12
Helixi221 – 229Combined sources9
Helixi234 – 248Combined sources15
Beta strandi253 – 263Combined sources11
Helixi264 – 266Combined sources3
Beta strandi268 – 279Combined sources12
Beta strandi282 – 286Combined sources5
Helixi293 – 310Combined sources18
Turni311 – 313Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LMRX-ray2.45A/B1-314[»]
4LN1X-ray1.90A/B/C/D1-314[»]
ProteinModelPortaliQ81EP4
SMRiQ81EP4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 126Substrate bindingUniRule annotation4
Regioni151 – 154Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
KOiK00016
OMAiDQWKNIH

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q81EP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKGINRVVL VGTGAVGCSY AYCMINQAVA EEFVLVDVNE AKAEGEAMDL
60 70 80 90 100
SHAVPFAPAP TRVWKGSYED CKDADLVVIT AGLPQKPGET RLDLVEKNAK
110 120 130 140 150
IFKQIVRSIM DSGFDGIFLI ATNPVDILTY VTWKESGLPK ERVIGSGTTL
160 170 180 190 200
DSARFRYMLG EYFDIGPHNI HAYIIGEHGD TELPVWSHVS VGIQKLQTLL
210 220 230 240 250
EKDNTYNQED LDKIFINVRD AAYHIIERKG ATYYGIGMSL LRVTKAILND
260 270 280 290 300
ENSVLTVSAY LEGQYGQKDV YIGVPAVLNR GGVREILEVE LSEDEELKFD
310
HSVQVLKETM APVL
Length:314
Mass (Da):34,789
Last modified:June 1, 2003 - v1
Checksum:i19DC3B1C2083AB2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA Translation: AAP08895.1
RefSeqiNP_831694.1, NC_004722.1
WP_000715321.1, NC_004722.1

Genome annotation databases

EnsemblBacteriaiAAP08895; AAP08895; BC_1924
GeneIDi1204273
31630598
KEGGibce:BC1924
PATRICifig|226900.8.peg.1927

Similar proteinsi

Entry informationi

Entry nameiLDH1_BACCR
AccessioniPrimary (citable) accession number: Q81EP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health