Q81EK4 (KHSE_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BC_1966

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_0000156549
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q81EK4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 84CEEF0371DBC500	FASTA	297	32,116
	10 20 30 40 50 60 MIPLSVRVPA STANVGPGFD SVGIALSLYL DVVVKEKADK WQVIHSFEES IPTDDKNLIV 70 80 90 100 110 120 STACKVCPSI SPHIIEVTSN IPLTRGLGSS ASAIVAGIEL ANQLGNLNLT ADQKVQIATN 130 140 150 160 170 180 FEGHPDNVAA SILGGTVIGA LDGKDISVVR IESKELGVIS LIPNEELNTD ESRSVLPKMF 190 200 210 220 230 240 PFHEAVKASA ISNVLVAALC QKRWEVVGEM MERDHFHEPY RLELVPLLPS IRKCAKEFGA 250 260 270 280 290 YGTALSGAGP SIFILTPYEK RQEIAEQLAR VFTDMKVCEL EIDHKGIIVN KEEHIGS

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08937.1.
RefSeq	NP_831736.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81EK4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81EK4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000031554; EBBACP00000030760; EBBACG00000031545.
GeneID	1204315.
GenomeReviews	Gene locus BC_1966 in contig AE016877_GR.
KEGG	bce:BC1966.
PATRIC	32599727. VBIBacCer54481_1972.
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000000874.
HOGENOM	HBG646290.
OMA	DRLHQPY.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	BCER226900:BC_1966-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACCR

Accession

Primary (citable) accession number: Q81EK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program