Q81EF6 (NAMA_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BC_2023

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.
Sequence caution	The sequence AAP08992.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADPH dehydrogenase HAMAP MF_01614		PRO_0000216114
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81EF6 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 77A2907FB25678FD	FASTA	345	38,582
	10 20 30 40 50 60 MNSKLFSPYT IKNVTLKNRI VMSPMCMYSS GNEDGRVTNF HLIHYGTRAA GQVGLVMVEA 70 80 90 100 110 120 TAVLAEGRIS NNDLGIWDDN LIEGLHKTTT FIHDNGAKAA IQLAHAGRKA ELDTNAFAPS 130 140 150 160 170 180 AIPFNDKMKI PVEMNIQQIK ETILAFQRAA LRSKQAGFDV IELHGAHGYL INEFLSPLTN 190 200 210 220 230 240 KRTDKYGGSP ENRYRFLREI IDSVNEVWDG PIFVRISAND YHPDGLTVQD YVQYTKWMKE 250 260 270 280 290 300 QGIDLIDCSS GAVVPAHIDV YPGYQVQYAK HIKEHTNIAT GAVGLITTGS QAEQILNNNE 310 320 330 340 ADLIFIGREL LRNPYFPRIA ANELGFELQE PHQYKRAPGK IHTNK

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP08992.1. Different initiation.
RefSeq	NP_831791.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q81EF6.
SMR	Q81EF6. Positions 4-337.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81EF6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000032558; EBBACP00000031764; EBBACG00000032549.
GeneID	1204372.
GenomeReviews	Gene locus BC_2023 in contig AE016877_GR.
KEGG	bce:BC2023.
PATRIC	32599848. VBIBacCer54481_2032.
Phylogenomic databases
eggNOG	COG1902.
GeneTree	EBGT00050000002219.
HOGENOM	HBG583461.
OMA	WVEDGWN.
PhylomeDB	Q81EF6.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	BCER226900:BC_2023-MONOMER.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00356.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACCR

Accession

Primary (citable) accession number: Q81EF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program