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Q81E30

- SYI2_BACCR

UniProt

Q81E30 - SYI2_BACCR

Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei593 – 5931ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBCER226900:GJEU-2164-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetase 2UniRule annotation
    Short name:
    IleRS 2UniRule annotation
    Gene namesi
    Name:ileS2UniRule annotation
    Ordered Locus Names:BC_2164
    OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
    Taxonomic identifieri226900 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000001417: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10331033Isoleucine--tRNA ligase 2PRO_0000098517Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi226900.BC2164.

    Structurei

    3D structure databases

    ProteinModelPortaliQ81E30.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi590 – 5945"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKWIISEI.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q81E30-1 [UniParc]FASTAAdd to Basket

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    MEKVDVKESA VGREMRIRKQ WNEQNIFEQS IQNREGAQSF VFYEGPPTAN     50
    GLPHVGHALG RTIKDLVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG 100
    ISGKHEIEEY GIEPFIQKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL 150
    KNPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV 200
    KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQES 250
    HVYIVAKERV QEVLKENYEV LSVHKGEELL NTSYTAPFPM KEVTNGYRVI 300
    AADFVTGDSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP 350
    FLKGKFVKDC DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE 400
    SWLIRTTAIK DTFLQNNDSV TWYPDHMKHG RFGKFLENMV DWNISRNRYW 450
    GTPLNVWECE SCDHQFAPKS IAELRKHSTK ETPEDLELHK PYVDEVQVCC 500
    GKCGGTMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG 550
    IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 600
    PVDLVDKFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLVNVYS 650
    FYVLYANLDE YNPKETYDVK LTKLDEWVLS RLHSTTKKVR TALDDYQFTN 700
    AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HEVLVTISKL 750
    IAPFTPFVAE DIHLNLEGSS VHLADYPVVN ESLLQPKLEA EMDAVLQVVE 800
    LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKAFHV 850
    ELDETKYTSY QLKLNFKTAG PKFGKNVNAV NGWLKQLSQE EVQNFVSTGR 900
    AVYEATPEGE VVVTGEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ 950
    EGVAREFIRA VQEYRKQLNL PVNLRVDIIL DTEEELQRTL TNHKDLLEEN 1000
    LLVKQFTFGH LTNEDDELSL GETKLRIKLS AAN 1033
    Length:1,033
    Mass (Da):118,308
    Last modified:December 20, 2005 - v2
    Checksum:i3BDB95615706F6A2
    GO

    Sequence cautioni

    The sequence AAP09130.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016877 Genomic DNA. Translation: AAP09130.1. Different initiation.
    RefSeqiNP_831929.1. NC_004722.1.

    Genome annotation databases

    EnsemblBacteriaiAAP09130; AAP09130; BC_2164.
    GeneIDi1204513.
    KEGGibce:BC2164.
    PATRICi32600157. VBIBacCer54481_2186.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016877 Genomic DNA. Translation: AAP09130.1 . Different initiation.
    RefSeqi NP_831929.1. NC_004722.1.

    3D structure databases

    ProteinModelPortali Q81E30.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226900.BC2164.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP09130 ; AAP09130 ; BC_2164 .
    GeneIDi 1204513.
    KEGGi bce:BC2164.
    PATRICi 32600157. VBIBacCer54481_2186.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KWIISEI.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BCER226900:GJEU-2164-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14579 / DSM 31.

    Entry informationi

    Entry nameiSYI2_BACCR
    AccessioniPrimary (citable) accession number: Q81E30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3