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Q81E30

- SYI2_BACCR

UniProt

Q81E30 - SYI2_BACCR

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Protein
Isoleucine--tRNA ligase 2
Gene
ileS2, BC_2164
Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBCER226900:GJEU-2164-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 2
Short name:
IleRS 2
Gene namesi
Name:ileS2
Ordered Locus Names:BC_2164
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001417: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Isoleucine--tRNA ligase 2UniRule annotation
PRO_0000098517Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi226900.BC2164.

Structurei

3D structure databases

ProteinModelPortaliQ81E30.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q81E30-1 [UniParc]FASTAAdd to Basket

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MEKVDVKESA VGREMRIRKQ WNEQNIFEQS IQNREGAQSF VFYEGPPTAN     50
GLPHVGHALG RTIKDLVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG 100
ISGKHEIEEY GIEPFIQKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL 150
KNPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV 200
KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQES 250
HVYIVAKERV QEVLKENYEV LSVHKGEELL NTSYTAPFPM KEVTNGYRVI 300
AADFVTGDSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP 350
FLKGKFVKDC DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE 400
SWLIRTTAIK DTFLQNNDSV TWYPDHMKHG RFGKFLENMV DWNISRNRYW 450
GTPLNVWECE SCDHQFAPKS IAELRKHSTK ETPEDLELHK PYVDEVQVCC 500
GKCGGTMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG 550
IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 600
PVDLVDKFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLVNVYS 650
FYVLYANLDE YNPKETYDVK LTKLDEWVLS RLHSTTKKVR TALDDYQFTN 700
AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HEVLVTISKL 750
IAPFTPFVAE DIHLNLEGSS VHLADYPVVN ESLLQPKLEA EMDAVLQVVE 800
LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKAFHV 850
ELDETKYTSY QLKLNFKTAG PKFGKNVNAV NGWLKQLSQE EVQNFVSTGR 900
AVYEATPEGE VVVTGEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ 950
EGVAREFIRA VQEYRKQLNL PVNLRVDIIL DTEEELQRTL TNHKDLLEEN 1000
LLVKQFTFGH LTNEDDELSL GETKLRIKLS AAN 1033
Length:1,033
Mass (Da):118,308
Last modified:December 20, 2005 - v2
Checksum:i3BDB95615706F6A2
GO

Sequence cautioni

The sequence AAP09130.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016877 Genomic DNA. Translation: AAP09130.1. Different initiation.
RefSeqiNP_831929.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP09130; AAP09130; BC_2164.
GeneIDi1204513.
KEGGibce:BC2164.
PATRICi32600157. VBIBacCer54481_2186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016877 Genomic DNA. Translation: AAP09130.1 . Different initiation.
RefSeqi NP_831929.1. NC_004722.1.

3D structure databases

ProteinModelPortali Q81E30.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226900.BC2164.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP09130 ; AAP09130 ; BC_2164 .
GeneIDi 1204513.
KEGGi bce:BC2164.
PATRICi 32600157. VBIBacCer54481_2186.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BCER226900:GJEU-2164-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14579 / DSM 31.

Entry informationi

Entry nameiSYI2_BACCR
AccessioniPrimary (citable) accession number: Q81E30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi