Reviewed, UniProtKB/Swiss-Prot Q81DR5 (IOLA_BACCR)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating]
      Short name=MMSA dehydrogenase
      Short name=MMSDH
      Short name=MSDH
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating]
      Short name=MSA dehydrogenase
    EC=1.2.1.18

Gene names

Name:	iolA
Ordered Locus Names:	BC_2290

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Methylmalonate semialdehyde dehydrogenase [acylating] HAMAP MF_01670		PRO_0000352321
Regions
	Nucleotide binding	178 – 182	5	NAD By similarity
Sites
	Active site	286	1	Nucleophile By similarity
	Binding site	386	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81DR5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 82FC20D727E4B83A	FASTA	486	52,954
	10 20 30 40 50 60 MITTEIKRVK NHINGEWVES TGTEVEAVPN PATGKIIAYV PLSPKEDVEK AVEAAKAAFE 70 80 90 100 110 120 TWSKVPVPNR SRNLYKYLQL LQENKDELAK IITLENGKTL TDATGEVQRG IEAVELATST 130 140 150 160 170 180 PNLMMGQALP NIASGIDGSI WRYPIGVVAG ITPFNFPMMI PLWMFPLAIA CGNTFVLKTS 190 200 210 220 230 240 ERTPLLAERL VELFYEAGFP KGVLNLVQGG KDVVNSILEN KDIQAVSFVG SEPVARYVYE 250 260 270 280 290 300 TGTKHGKRVQ ALAGAKNHAI VMPDCNLEKT VQGVIGSAFA SSGERCMACS VVAVVDEIAD 310 320 330 340 350 360 EFIDVLVAET KKLKVGDGFN EDNYVGPLIR ESHKERVLGY INSGVADGAT LLVDGRKINE 370 380 390 400 410 420 EVGEGYFVGA TIFDGVNQEM KIWQDEIFAP VLSIVRVKDL EEGIKLTNQS KFANGAVIYT 430 440 450 460 470 480 SNGKHAQTFR DNIDAGMIGV NVNVPAPMAF FAFAGNKASF FGDLSTNGTD GVQFYTRKKV VTERWF

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP09254.1.
RefSeq	NP_832053.1.
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Genome annotation databases
GeneID	1204639.
GenomeReviews	Gene locus BC_2290 in contig AE016877_GR.
KEGG	bce:BC2290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81DR5.
OMA	Q81DR5. KVGPGNG.
Enzyme and pathway databases
BioCyc	BCER226900:BC_2290-MONOMER.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA_BACCR

Accession

Primary (citable) accession number: Q81DR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)