Reviewed, UniProtKB/Swiss-Prot Q81D33 (DDLA_BACCR)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase A
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase A
    D-Ala-D-Ala ligase A

Gene names

Name:	ddlA
Ordered Locus Names:	BC_2550

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	D-alanine--D-alanine ligase A HAMAP MF_00047		PRO_0000177782
Regions
	Domain	96 – 290	195	ATP-grasp
	Nucleotide binding	123 – 178	56	ATP By similarity
Sites
	Metal binding	245	1	Magnesium or manganese 1 By similarity
	Metal binding	257	1	Magnesium or manganese 1 By similarity
	Metal binding	257	1	Magnesium or manganese 2 By similarity
	Metal binding	259	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81D33-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4F3304CD90438BFE	FASTA	301	33,595
	10 20 30 40 50 60 MLLWAGYASE KQVSIMTGNE MIAHLDKNKY EIVPITLNEK MDLIEKAKDI DFALLALHGK 70 80 90 100 110 120 YGEDGTVQGT LESLGIPYSG SNMLSSSICM DKNISKKILR YEGVETPDWI ELTKMEDLNL 130 140 150 160 170 180 DELDKLGFPL VVKPNSGGSS VGVKIVYNKN ELISMLETVF EWDSEVVIEK YIKGDEITCS 190 200 210 220 230 240 ILDGKQLPIV SIRHAAEFFD YNAKYDDTST VEEVIELPAQ IKERVNKASL TCYKALKCSV 250 260 270 280 290 300 YARVDMMVKD GIPYVMEINT LPGMTQSSLL PKSAEAAGIS YSKLLDMIIE TSLKVRNEEG F

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP09509.1.
RefSeq	NP_832308.1.
3D structure databases
HSSP	HSSP built from PDB template 1IOW based on UniProtKB P07862.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81D33.
Genome annotation databases
GeneID	1204899.
GenomeReviews	Gene locus BC_2550 in contig AE016877_GR.
KEGG	bce:BC2550.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81D33.
OMA	KGDEITC.
Enzyme and pathway databases
BioCyc	BCER226900:BC_2550-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLA_BACCR

Accession

Primary (citable) accession number: Q81D33

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)