Reviewed, UniProtKB/Swiss-Prot Q81AC8 (HUTI_BACCR)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BC_3650

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Imidazolonepropionase HAMAP MF_00372		PRO_0000306430
Sites
	Metal binding	78	1	Zinc or iron By similarity
	Metal binding	80	1	Zinc or iron By similarity
	Metal binding	247	1	Zinc or iron By similarity
	Metal binding	322	1	Zinc or iron By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q81AC8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FFA030EBD0CD8AE7	FASTA	423	46,211
	10 20 30 40 50 60 MLDTLLINIG QLLTMDQEDG LLRREAMNTL PVIENGVVGI ENGVITFVGT AEEAKGLQAK 70 80 90 100 110 120 EVIDSGGKMV SPGLVDPHTH LVFGGSRENE IALKLQGVPY LEILEQGGGI LSTVNATKQA 130 140 150 160 170 180 SKEELVQKAK FHLDRMLSFG VTTVEAKSGY GLDDETEWKQ LEATAQLQKE HPIDLVSTFL 190 200 210 220 230 240 GAHAVPKEYK GRSKEFLQWM LDLLPEMKEK QLVEFVDIFC ETGVFSVEES KEFLLKAKEL 250 260 270 280 290 300 GFDVKIHADE IDPLGGAEAA AEIGAASADH LVGASDKGIE MLANSNTVAT LLPGTTFYLN 310 320 330 340 350 360 KESFARGRKM IDEGVAVALA TDFNPGSCPT ENIQLIMSIA MLKLKMTPEE VWNAVTVNSS 370 380 390 400 410 420 YAINRGDVAG KIRVGRKADL VLWDAYNYAY VPYHYGVSHV NTVWKNGNIA YTRGEQSWST ATI

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP10579.1.
RefSeq	NP_833378.1.
3D structure databases
SMR	Q81AC8. Positions 2-413.
ModBase	Search...
Protein-protein interaction databases
STRING	Q81AC8.
Genome annotation databases
GeneID	1205995.
GenomeReviews	Gene locus BC_3650 in contig AE016877_GR.
KEGG	bce:BC3650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q81AC8.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3650-MON.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BACCR

Accession

Primary (citable) accession number: Q81AC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)